Collection of T-DNA insert ional mutant lines of Micro-Tom and screening of essential genes for various phenotypes

• Project/Area Number: 21510208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: University of Tsukuba
• Principal Investigator: MIZOGUCHI Tsuyoshi 筑波大学, 生命環境系, 准教授 (70281623)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 遺伝学 / 植物 / 発生 / 分化 / 発生・分化

Research Abstract

In this work, we generated T-DNA insertional lines of Micro-Tom with Agrobacterium. Phenotypic analyses of the tomato plants with T-DNA insertions were performed under several different photoperiodic conditions. Molecular genetic analysis was also done to estimate numbers of genes required for visible phenotypes found in the Micro-Tom mutants.

Research Products

• [Journal Article] Suppression of late-floweringand semi-dwarf phenotypes in the Arabidopsis clock mutant lhy-12 ; cca1-101 by phyB under continuous light (2011)
  • Author(s): Miyata K, Calvino M, Oda A, Sugiyama H, Mizoguchi T
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1161-1171
  • Peer Reviewed
• [Journal Article] A trial of production of the plant-derived high-value protein in a plant factory: Photosynthetic photon fluxes affect the accumulation of recombinant miraculin in transgenic tomato fruits (2011)
  • Author(s): Kato K, Maruyama S, Hirai T, Hiwasa-Tanase K, Mizoguchi T, Goto E, Ezura H
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1172-1179
  • Peer Reviewed
• [Journal Article] Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Bres C, Rothan C, Mizoguchi T, Ezura H
  • Journal Title: Plant Cell Physiology Volume: 52 Pages: 1994-2005
  • NAID: 10030305636
  • Peer Reviewed
• [Journal Article] Double loss-of-function mutation in EARLY FLOWERING 3 and CRYPTOCHROME 2 genes delays flowering under continuous light but accelerates it under long-days and short-days : An important role of Arabidopsis CRY2 to accelerate flowering time in continuous lig (2011)
  • Author(s): Nefissi R, Natsui Y, Miyata K, Oda A, Hase Y, Nakagawa M, Ghorbel A, Mizoguchi T
  • Journal Title: Journal of Experimental Botany Volume: 62 Pages: 2731-2744
  • Peer Reviewed
• [Journal Article] TOMATOMA : A novel tomato mutant database distributing Micro-Tom mutant collections (2011)
  • Author(s): Saito T, Ariizumi T, Okabe Y, Asamizu E, Hiwasa-Tanase K, Fukuda N, Mizoguchi T, Yamazaki Y, Aoki K, Ezura H
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 283-296
  • NAID: 10029708660
  • Peer Reviewed
• [Journal Article] Suppression of Late-Flowering and Semi-Dwarf Phenotypes in the arabidopsis Clock Mutant lhy-12;cca1-101 by phyB under Continuous Light (2011)
  • Author(s): Miyata K., Calvino M., Oda A., Sugiyama H., Mizoguchi T.
  • Journal Title: Plant Signal Behav Volume: 6 Issue: 8 Pages: 1162-1171
  • DOI: 10.4161/psb.6.8.16361
  • Peer Reviewed
• [Journal Article] Double loss-of-function mutation in EARLY FLOWERING 3 and CRYPTOCHRO ME 2 genes delays flowering under continuous light but accelerates it under long-days and short-days : An important role of Arabidopsis CRY2 to accelerate flowering time in continuous light. (2011)
  • Author(s): Nefissi R, Natsui Y, Miyata K, Oda A, Hase Y, Nakagawa M, Ghorbel A, Mizoguchi T*
  • Journal Title: Journal of Experimental Botany
  • Peer Reviewed
• [Journal Article] TOMATOMA : A novel tomato mutant database distributing Micro-Tom mutant collections. (2011)
  • Author(s): Saito T, Ariizumi T, Okabe Y, Asamizu E, Hiwasa-Tanase K, Fukuda N, Mizoguchi T, Yamazaki Y, Aoki K, Ezura H*
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 283-296
  • NAID: 10029708660
  • Peer Reviewed
• [Journal Article] Molecular breeding of tomato lines for mass production of miraculin in a plant factory (2010)
  • Author(s): Kato K, Yoshida R, Kikuzaki A, Hirai T, Kuroda H, Hiwasa-Tanase K, Takane K, Ezura H, Mizoguchi T
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 58 Pages: 9505-9510
  • Peer Reviewed
• [Journal Article] Molecular breeding of tomato lines for mass production of miraculin in a plant factory. (2010)
  • Author(s): Kato K, Yoshida R, Kikuzaki A, Hirai T, Kuroda H, Hiwasa-Tanase K, Takane K, Ezura H, Mizoguchi T*
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 58 Pages: 9505-9510
  • Peer Reviewed
• [Journal Article] 長日植物シロイヌナズナと中性植物トマトにおける花成制御と花形態形成に関わる遺伝子群 (2009)
  • Author(s): 加藤一幾、溝口剛
  • Journal Title: 植物の生長調節 44 Pages: 111-117
• [Presentation] Inversion of photoperiodic response by clock mutations in Arabidopsis (2011)
  • Author(s): Mizoguchi T
  • Organizer: The 11^<th> AEARU International Workshop on Molecular Biology and Biotechnology
  • Place of Presentation: Hong Kong
• [Presentation] Photoperiodic Flowering Controlled by Circadian Clock and Light/Dark Cycles (2011)
  • Author(s): Mizoguchi T
  • Organizer: Symposium for Annual meeting of Korean Society of Molecular and Cellular Biology (招待講演)
  • Place of Presentation: Seoul, Korea
• [Presentation] SVP and BZR1 Integrate Brassinosteroid Signaling and Circadian Clock to Regulate Flowering Time and Body Size in Arabidopsis, Interplay of Light (2011)
  • Author(s): Mizoguchi T
  • Organizer: Photoperiodic and Circadian Clock Function in Plant Development (招待講演)
  • Place of Presentation: Barcelona, Spain
• [Presentation] Characterization of a natural variation between Ws and Ler that may affect expression of floral repressors FLC and MAF2-5 in the clock mutant lhy ; cca1 (2011)
  • Author(s): Nefissi R, Natsui Y, Fujiwara S, Suzuki S, Hara M, Mizoguchi T
  • Organizer: 2010年度日本植物生理学会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] Characterization of a natural variation between Ws and Ler that may affect expression of floral repressors FLC and MAF2-5 in the clock mutant lhy;ccal. (2011)
  • Author(s): Nefissi R, Natsui Y, Fujiwara S, Suzuki S, Hara M, Mizoguchi T*
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] 概日時計因子LHYとCCA1によるクロロフィル量及びクロロフィルa/b比の制御 (2010)
  • Author(s): 宮田佳奈、渥美太朗、溝口剛
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-20