Large DNA molecule introduction into animal, plant and yeast cells using Type IV secretion system
Project/Area Number |
21510209
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied genomics
|
Research Institution | Hiroshima University |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | DNA レベルの生物間相互作用 / 四型分泌系 / 生物界間接合 / イネOc培養細胞 / タバコBY-2培養細胞 / タマネギ表皮細胞 / 接合用弱毒性大腸菌株 / 弱毒性大腸菌 / ベクターの再環状化 / oriT / DNA修復系 |
Research Abstract |
One of Type IV secretion systems (T4SS), which is derived from an IncP type plasmid, was applied as a novel gene introduction method into eukaryotic cells. We found that mitochondrial respiratory mutants in S. cerevisiae showed enhanced transformation efficiency. By using inhibitors of mitochondrial function, we successfully established a simple and efficient gene introduction method into S. cerevisiae from E. coli. In addition, we firstly observed direct gene transfer from E.coli to higher plant cells by applying this T4SS.
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Report
(4 results)
Research Products
(11 results)