| Project/Area Number |
21510227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Ehime University |
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Principal Investigator |
TAMURA Minoru 愛媛大学, 大学院・理工学研究科, 准教授 (00128349)
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| Co-Investigator(Renkei-kenkyūsha) |
SUMOMOTO Hideki 九州大学, 大学院・医学研究科, 教授 (30179303)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 酵素 / 生体生命情報学 / 発生分化 / シグナル伝達 / 活性酸素 / スーパーオキシド / スーバーオキシド |
|---|
| Research Abstract |
We established the method for production of Noxa1, an essential activating factor for Nox1 using E. coli. We also succeeded to express and purifyβPix andβ-actin, which may influence the activation of Nox1. We examined the cell-free activation of Nox1 using Caco-2 plasma membrane as an enzyme source. We also clarified the kinetic properties of Noxa1 in a pure cell-free activation system containing Nox2 purified from porcine neutrophils. The model cell system of Nox1 was established using HEK293 cells transfected with Nox1 cDNA as well as cDNAs for Noxa1 and Noxo1. The effect ofβPix, a putative GEF for Rac, on the activation of Nox enzymes was examined in cell and cell-free system.
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