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Mechanisms for activation and signaling of NADPH oxidase 1 involved in cell proliferation

Research Project

Project/Area Number 21510227
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionEhime University

Principal Investigator

TAMURA Minoru  愛媛大学, 大学院・理工学研究科, 准教授 (00128349)

Co-Investigator(Renkei-kenkyūsha) SUMOMOTO Hideki  九州大学, 大学院・医学研究科, 教授 (30179303)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords酵素 / 生体生命情報学 / 発生分化 / シグナル伝達 / 活性酸素 / スーパーオキシド / スーバーオキシド
Research Abstract

We established the method for production of Noxa1, an essential activating factor for Nox1 using E. coli. We also succeeded to express and purifyβPix andβ-actin, which may influence the activation of Nox1. We examined the cell-free activation of Nox1 using Caco-2 plasma membrane as an enzyme source. We also clarified the kinetic properties of Noxa1 in a pure cell-free activation system containing Nox2 purified from porcine neutrophils. The model cell system of Nox1 was established using HEK293 cells transfected with Nox1 cDNA as well as cDNAs for Noxa1 and Noxo1. The effect ofβPix, a putative GEF for Rac, on the activation of Nox enzymes was examined in cell and cell-free system.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (9 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Noxa1 as a moderate activator of Nox2-based NADPH oxidase2012

    • Author(s)
      Masahito Kawano, Kazuaki Miyamoto, Yuki Kaito, Hideki Sumimoto, and Minoru Tamura
    • Journal Title

      Arch. Biochem. Biophys.

      Volume: Vol.519, No.1 Pages: 1-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Noxa1 as a moderate activator of Nox2-based NADPH oxidase2012

    • Author(s)
      Kawano M, Miyamoto K, Kaito Y, Sumimoto H, Tamura M
    • Journal Title

      Arch. Biochem. Biophys

      Volume: 519 Issue: 1 Pages: 1-7

    • DOI

      10.1016/j.abb.2011.12.025

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of humanβ-actin and a mutant using a bacterial expression system with a cold shock vector2011

    • Author(s)
      Minoru Tamura, Katsunori Ito, Sachio Kunihiro, Chihoko Yamasaki, and Mihoko Haragauchi
    • Journal Title

      Prot. Expr. Purifi.

      Volume: Vol.78, No.1 Pages: 1-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of human β-actin and a mutant using a bacterial expression system with a cold shock vector2011

    • Author(s)
      Minoru Tamura
    • Journal Title

      Protein Expr.Purif.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TNF受容体シグナル伝達の新しいかたち2009

    • Author(s)
      田村実
    • Journal Title

      蛋白質・核酸・酵素

      Volume: 54巻 Pages: 1878-1878

    • Related Report
      2011 Final Research Report
  • [Journal Article] TNF受容体シグナル伝達の新しいかたち2009

    • Author(s)
      田村実
    • Journal Title

      蛋白質・核酸・酵素 54

      Pages: 1878-1878

    • Related Report
      2009 Annual Research Report
  • [Presentation] NADPH oxidase活性化におけるβ-Pixの役割2011

    • Author(s)
      階戸悠貴、河野真仁、原垣内美保子、田村実
    • Organizer
      日本生化学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Nox1活性化因子NoxA1の精製と性質-2量体体形成の可能性-2010

    • Author(s)
      河野真仁、濱島侑紀、中島舞子、竹内一弘、田村実
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] HEK293細胞に対するROSの影響-高性能O_2^-発生ツールを用いて2010

    • Author(s)
      濱島侑紀、階戸悠貴、竹内一弘、田村実
    • Organizer
      日本生化学会・分子生物学合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Nox1活性化因子NoxA1の精製と性質-二量体形成の可能性2010

    • Author(s)
      河野真仁
    • Organizer
      日本生化学会・分子生物学会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] HEK293細胞に対するROSの影響-高性能O_2発生ツールを用いて2010

    • Author(s)
      濱島侑紀
    • Organizer
      日本生化学会・分子生物学会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 全長Noxa1およびNoxo1の大量発現精製とNADPH oxidase活性化能2009

    • Author(s)
      田村実、宮本和浩、濱島侑紀、森岡絵梨奈、重松隆
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 全長Noxa1およびNoxo1の大量発現とNADPH oxidase 活性化能2009

    • Author(s)
      田村実
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] NoxA1の大腸菌による発現精製とNox1活性化能2009

    • Author(s)
      宮本和浩、重松隆、濱島侑紀、森岡絵梨奈、田村実
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-22
    • Related Report
      2011 Final Research Report
  • [Presentation] NoxA1の大腸菌による発現精製とNox活性化能2009

    • Author(s)
      宮本和浩
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ehime-u.ac.jp/~achem/biotec/index.html/index.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.ehime-u.ac.jp/~achem/biotec/index.html/index.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] アクチンの製造方法、それに用いるベクターおよび原核宿主細胞2011

    • Inventor(s)
      田村実、伊藤克法
    • Industrial Property Rights Holder
      国立大学法人愛媛大学
    • Filing Date
      2011-12-28
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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