| Project/Area Number |
21510230
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
|
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | セルラーゼ / 細胞壁 / 形態形成 / バイオマス / 転写因子 / 細胞性粘菌 |
|---|
| Research Abstract |
Cellulose is a major component of the cell wall for cellular slime mould, Dictyostelium discoideum. It posses a cellulose synthetase gene and its function is important for normal morphogenesis. In this project, we focused on the cellulase genes catalyzing cellulose hydrolysis, cloned several of them and investigated the effects on morphogenesis as well as cellulose hydrolyzing activity. We showed inactivation of cellobiohydrolase(cbhA) gene, one of cellulase, by gene disruption affected morphogenesis. In addition, multiple Dictyostelium cellulases might act synergistically, possibly as a cellulosome, resulted in drastic increase of cellulose hydrolyzing activity. This implies Dictyostelium is a useful organism to produce bioethanol from cellulose.
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