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Cloning of cDNAs encoding enzymes involved in the natural product biosynthesis of leguminous plants using functional expression screening.

Research Project

Project/Area Number 21510232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionNihon University

Principal Investigator

AKASHI Tomoyoshi  日本大学, 生物資源科学部, 講師 (80328707)

Co-Investigator(Renkei-kenkyūsha) AOKI Toshio  日本大学, 生物資源科学部, 准教授 (80287606)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords生合成 / ファイトアレキシン / イソフラボノイド / プレニル基転移酵素 / フラボノイド / P450 / テルペノイド
Research Abstract

More than 90% of isoflavonoids are present in the leguminous plants. In the leguminous plants, isoflavonoids are typical ecophysiologicallyactive compounds such as defense substances and signal molecules in the early stages of symbiosis with rhizobial bacteria. In this study, we identified several cDNAs encoding enzymes in isoflavonoid pathway by screening of cDNA expression library. A pilot experiment of biotechnological production of isoflavonoids was examined.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (14 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] イソフラボノイド生合成酵素遺伝子のディスカバリーから見えてきた多様性2011

    • Author(s)
      明石智義
    • Journal Title

      BSJReview

      Volume: Vol.2 Pages: 18-23

    • Related Report
      2011 Final Research Report
  • [Journal Article] イソフラボノイド生合成酵素遺伝子のディスカバリーから見えてきた多様性2011

    • Author(s)
      明石智義
    • Journal Title

      BSJ-Review

      Volume: 2 Pages: 18-23

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Plant Phenolics : Phenylpropanoids2010

    • Author(s)
      Ayabe, S., Uchiyama, S., Aoki, T.Akashi, T.
    • Journal Title

      Comprehensive Natural Products II : Chemistry and Biology

      Volume: Volume 1 Pages: 929-976

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ダイズのファイトアレキシン生合成系の新規プレニル基転移酵素遺伝子2012

    • Author(s)
      米山恵介, 中川剛, 青木俊夫, 明石智義
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イソフラボノイド生合成酵素遺伝子のディスカバリーから見えてきた多様性2011

    • Author(s)
      明石智義
    • Organizer
      「生合成マシナリー」第3回公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-12-03
    • Related Report
      2011 Final Research Report
  • [Presentation] イソフラボノイド生合成酵素遺伝子のディスカバリーから見えてきた多様性2011

    • Author(s)
      明石智義
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-19
    • Related Report
      2011 Final Research Report
  • [Presentation] アヤメ属由来2一ヒドロキシイソフラバノン合成酵素cDNAを用いたシロイヌナズナでのイソフラボノイド生産2011

    • Author(s)
      吉田隼, 青木俊夫, 明石智義
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-19
    • Related Report
      2011 Final Research Report
  • [Presentation] アヤメ属由来2-ヒドロキシイソフラバノン合成酵素cDNAを用いたシロイヌナズナでのイソフラボノイド生産2011

    • Author(s)
      吉田隼, 青木俊夫, 明石智義
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] イソフラボノイド生合成酵素遺伝子のディスカバリーから見えてきた多様性2011

    • Author(s)
      明石智義
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 単子葉アヤメ属植物のイソフラボノイド骨格合成酵素遺伝子2011

    • Author(s)
      明石智義, 吉田隼, 青木俊夫
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会・シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 単子葉アヤメ属植物のイソフラボノイド骨格合成酵素遺伝子2011

    • Author(s)
      明石智義, 吉田隼, 青木俊夫
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ダイズのイソフラボノイドファイトアレキシン生合成系の新規プレニル基転移酵素遺伝子2011

    • Author(s)
      米山恵介, 青木俊夫, 明石智義
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会・シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2011-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] ダイズのイソフラボノイドファイトアレキシン生合成系の新規プレニル基転移酵素遺伝子2011

    • Author(s)
      米山恵介, 青木俊夫, 明石智義
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] イソフラボノイド合成酵素の機能と分子進化2011

    • Author(s)
      明石智義
    • Organizer
      生合成勉強会第2回若手シンポジウム
    • Place of Presentation
      埼玉
    • Year and Date
      2011-07-10
    • Related Report
      2011 Final Research Report
  • [Presentation] イソフラボノイド合成酵素の機能と分子進化2011

    • Author(s)
      明石智義
    • Organizer
      新学術領域「生合成マシナリー」第2回若手シンポジウム
    • Place of Presentation
      埼玉
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isoflavonoid biosynthesis in Iris germanica(Iridaceae). Plant Biology 20102010

    • Author(s)
      Akashi, T., Aoki, T
    • Organizer
      Annual Meeting of the American Society of Plant Biologists
    • Place of Presentation
      カナダ,モントリオール
    • Year and Date
      2010-08-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Isoflavonoid biosynthesis in Iris germatuca(Iridaceae)2010

    • Author(s)
      Akashi, T., Aoki, T.
    • Organizer
      Plant Biology 2010, Annual Meeting of the American Society of Plant Biologists
    • Place of Presentation
      カナダ,モントリオール
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Book] Plant Phenolics : Phenylpropanoids, In Comprehensive Natural Products II : Chemistry and Biology, Volume 1 : Natural Products Structural Diversity-I Secondary Metabolites : Organization and Biosynthesis2010

    • Author(s)
      Ayabe, S., Uchiyama, S., Aoki, T. and Akashi, T.
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/hbunshi/seitai/Welcome.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~bunshi/seitai/Welcome.html

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] アヤメ属由来高活性イソフラボン骨格合成酵素を用いたイソフラボン生産技術2010

    • Inventor(s)
      青木俊夫,明石智義
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2010-226781
    • Filing Date
      2010-10-06
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] アヤメ属由来高活性イソフラボン骨格合成酵素を用いたイソフラボン生産技術2010

    • Inventor(s)
      青木俊夫, 明石智義
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Number
      2010-226781
    • Filing Date
      2010-10-06
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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