Cloning of cDNAs encoding enzymes involved in the natural product biosynthesis of leguminous plants using functional expression screening.
| Project/Area Number |
21510232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AOKI Toshio 日本大学, 生物資源科学部, 准教授 (80287606)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生合成 / ファイトアレキシン / イソフラボノイド / プレニル基転移酵素 / フラボノイド / P450 / テルペノイド |
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| Research Abstract |
More than 90% of isoflavonoids are present in the leguminous plants. In the leguminous plants, isoflavonoids are typical ecophysiologicallyactive compounds such as defense substances and signal molecules in the early stages of symbiosis with rhizobial bacteria. In this study, we identified several cDNAs encoding enzymes in isoflavonoid pathway by screening of cDNA expression library. A pilot experiment of biotechnological production of isoflavonoids was examined.
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Report
(4 results)
Research Products
(22 results)
