| Project/Area Number |
21510233
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Toshio 日本大学, 生物資源科学部, 准教授 (80287606)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIYAMA Hiroshi 日本大学, 生物資源科学部, 准教授 (40232871)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 二次代謝産物 / 植物形質転換 / フラボノイド / トリテルペノイド / ミヤコグサ / シオン / アカシア / 植物天然物 / 生合成 / 酵素 / 遺伝子 / 毛状根 / 二次代謝 |
|---|
| Research Abstract |
To clarify the mechanism that creates the vast structural diversity of plant natural products, cDNAs encoding the enzymes involved in the biosynthesis of flavonols of a leguminous model plant Lotus japonicus, a specific triterpenoid of Aster tataricus and polyphenols of Acacia mangium were cloned, and the enzyme reactions were analyzed. Also, metabolic engineering was attempted with L. japonicus hairy root systems in which the expression of genes of flavonoid pathway was altered. Furthermore, the phytoalexin response of the cultured L. japonicus cells against various microbe signals was analyzed in order to facilitate the engineering efficiencies.
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