Analysis for Dynamics on High Order Structure of Protein
Project/Area Number |
21540417
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics/Chemical physics
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Research Institution | Kyoto University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KATO Koichi 名古屋市立大学, 薬学研究科, 教授 (20211849)
MORIMOTO Yukio 京都大学, 原子炉実験所, 教授 (80200450)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 生物物理 / サブユニット交換 / 重水素化 / 中性子小角散乱 / コントラスト変調法 / タンパク質高次構造 |
Research Abstract |
We established an analysis method for dynamics of high order structure of protein by Small-Angle Neutron Scattering utilizing deuterated proteins and solvent contrast variation technique. With the method, we found the subunit exchange between the aggregates consisting ofα7-subunits which are one of subunits of the 20S proteasome, and analyzed its kinetics.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Assembly State of Proteasome Activator 28 in an Aqueous Solution as Studied by Small-Angle Neutron Scattering2009
Author(s)
M. Sugiyama, E. Kurimoto, Y. Morimoto, H. Sahashi, E. Sakata, K. Hamada, K. Itoh, K. Mori, T. Fukunaga, Y. Minami and K. Kato
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Journal Title
Journal of the Physical Society of Japan
Volume: 78
Issue: 12
Pages: 124802-124802
DOI
NAID
ISSN
0031-9015, 1347-4073
Related Report
Peer Reviewed
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