Analysis for Dynamics on High Order Structure of Protein

• Project/Area Number: 21540417
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Kyoto University
• Principal Investigator: SUGIYAMA Masaaki 京都大学, 原子炉実験所, 教授 (10253395)
• Co-Investigator(Kenkyū-buntansha): KATO Koichi 名古屋市立大学, 薬学研究科, 教授 (20211849) ; MORIMOTO Yukio 京都大学, 原子炉実験所, 教授 (80200450)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生物物理 / サブユニット交換 / 重水素化 / 中性子小角散乱 / コントラスト変調法 / タンパク質高次構造

Research Abstract

We established an analysis method for dynamics of high order structure of protein by Small-Angle Neutron Scattering utilizing deuterated proteins and solvent contrast variation technique. With the method, we found the subunit exchange between the aggregates consisting ofα7-subunits which are one of subunits of the 20S proteasome, and analyzed its kinetics.

Research Products

• [Journal Article] Kinetic asymmetry of subunit exchange of homooligomeric protein as revealed by deuteration-assisted small-angle neutron scattering. (2011)
  • Author(s): M.Sugiyama, E.Kurimoto, H.Yagi, K.Mori, T.Fukunaga, M.Hirai, G.Zaccai, and K.Kato
  • Journal Title: Biophys. J. Volume: 101 Issue: 8 Pages: 2037-2042
  • DOI: 10.1016/j.bpj.2011.09.004
  • NAID: 120003551148
  • Peer Reviewed
• [Journal Article] SAXS and SANS Observations of Abnormal Aggregation of Humanα-Crystallin (2010)
  • Author(s): M. Sugiyama, N. Fujii, Y. Morimoto, K. Itoh, K. Mori, T. Fukunaga and N. Fujii
  • Journal Title: Chemistry & Biodiversity Volume: 7No.6 Issue: 6 Pages: 1380-1386
  • DOI: 10.1002/cbdv.200900332
  • Peer Reviewed
• [Journal Article] SANS investigation of assembly state of proteasome activator 28 and the 20 Sproteasome (2010)
  • Author(s): M. Sugiyama, E. Kurimoto, H. Sahashi, E. Sakata, Y. Morimoto, K. Itoh, K. Mori, T. Fukunaga, Y. Minami and K. Kato
  • Journal Title: Journal of Physics Volume: 247 Pages: 12020-12020
  • Peer Reviewed
• [Journal Article] SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome (2010)
  • Author(s): M.Sugiyama, Y.Morimoto, K.Kato, 他7名
  • Journal Title: Journal of Physics Volume: 247 Pages: 12020-12020
  • Peer Reviewed
• [Journal Article] SAXS and SANS Observations of Abnormal Aggregation of Human α-Crystallin (2010)
  • Author(s): M.Sugiyama, Y.Morimoto, 他5名
  • Journal Title: CHEMISTRY & BIODIVERSITY Volume: 7 Pages: 1380-1388
  • Peer Reviewed
• [Journal Article] Assembly State of Proteasome Activator 28 in an Aqueous Solution as Studied by Small-Angle Neutron Scattering (2009)
  • Author(s): M. Sugiyama, E. Kurimoto, Y. Morimoto, H. Sahashi, E. Sakata, K. Hamada, K. Itoh, K. Mori, T. Fukunaga, Y. Minami and K. Kato
  • Journal Title: Journal of the Physical Society of Japan Volume: 78 Issue: 12 Pages: 124802-124802
  • DOI: 10.1143/jpsj.78.124802
  • NAID: 130005437296
  • ISSN: 0031-9015, 1347-4073
  • Peer Reviewed
• [Journal Article] Assembly State of Proteasome Activator 28 in an Aqueous Solution as Studied by Small-Angle Neutron Scattering (2009)
  • Author(s): M.Sugiyama, Y.Morimoto, K.Kato, (他8名)
  • Journal Title: Journal of the Physical Society of Japan 78 Pages: 124802-124802
  • NAID: 130005437296
  • Peer Reviewed
• [Presentation] Observation of Nano-Scale Structure of Protein with Deuteration. Assisted Small-Angle Neutron Scattering (2012)
  • Author(s): M. Sugiyama
  • Organizer: The 12th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 野依ホール名古屋大学
  • Year and Date: 2012-01-25
• [Presentation] Observation of Nano-Scale Structure of Protein with Deuteration-Assisted Small-Angle Neutron Scattering (2012)
  • Author(s): M.Sugiyama
  • Organizer: The 12th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 野依ホール(名古屋大学)(招待講演)
  • Year and Date: 2012-01-25
• [Presentation] High Order Structure of Protein and Its Kinetics with Deuteration-Assisted Small-Angle Neutron Scattering (2011)
  • Author(s): M. Sugiyama
  • Organizer: The International Conference on Advanced Materials and Nanotechnology
  • Place of Presentation: Park Village Resort Hotel, Kathmanduネパール
  • Year and Date: 2011-10-22
• [Presentation] High Order Structure of Protein and Its Kinetics with Deuteration-Assisted Small-Angle Neutron Scattering (2011)
  • Author(s): M.Sugiyama
  • Organizer: The International Conference on Advanced Materials and Nanotechnology
  • Place of Presentation: Park Village Resort Hotel, Kathmandu(ネパール)(招待講演)
  • Year and Date: 2011-10-22
• [Presentation] 野生型PA28におけるSubunit配置と解離 (2010)
  • Author(s): 杉山正明
  • Organizer: 第10回日本中性子科学会年会
  • Place of Presentation: 東北大学片平さくらホール
  • Year and Date: 2010-12-11
• [Presentation] Abnormal Aggregation of alpha-crystallin (2010)
  • Author(s): M. Sugiyama
  • Organizer: The 11th IUMRS-International Conference in Asia
  • Place of Presentation: Quingdao, China
  • Year and Date: 2010-09-26
• [Presentation] Abnormal Aggregation of alpha-crystallin (2010)
  • Author(s): M.Sugiyama
  • Organizer: The 11th IUMRS-Internation Conference in Asia
  • Place of Presentation: Quingdao, China
  • Year and Date: 2010-09-26
• [Presentation] Observation of Subunit Kinetics in Proteasome aτring with SANS and SAXS (2010)
  • Author(s): M. Sugiyama
  • Organizer: The Fourth Taiwan-Japan Joint Meeting on Neutron and X-ray Scattering
  • Place of Presentation: Yilan, Taiwan
  • Year and Date: 2010-03-09
• [Presentation] Observation of Subunit Kinetics in Proteasome a7 ring with SANS and SAXS (2010)
  • Author(s): M.Sugiyama
  • Organizer: The Fourth Taiwan-Japan Joint Meeting on Neutron and X-ray Scattering
  • Place of Presentation: Yilan, Taiwan
  • Year and Date: 2010-03-09
• [Presentation] プロテアソーム複合体におけるサブユニットキネティクスの研究 (2009)
  • Author(s): 杉山正明
  • Organizer: 日本中性子科学会第9回年会
  • Place of Presentation: いばらき量子ビーム研究センター
  • Year and Date: 2009-11-11
• [Presentation] SANS Investigation of Packing Structure of Proteasome Activator 28 with Deuterated Subunit (2009)
  • Author(s): M. Sugiyama
  • Organizer: XIV International Conference on Small-Angle Scattering
  • Place of Presentation: Oxford, United Kingdom
  • Year and Date: 2009-09-16