| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Research Abstract |
Facile and large-scale synthesis of stereoselectively stable isotope-labeled amino acids, which can promote the efficiency of NMR protein structure determination, was investigated. In this work, a novel synthetic method for unsaturated pyroglutaminol derivatives, key intermediates for eight kinds of labeled amino acids, has been developed and gram-scale syntheses of deuterated prolines and glutamic acids were achieved. Using the obtained proline, some dipeptides were prepared and a systematic conformational analysis was carried out. With the help of selective deuterium-labeling, it becomes feasible to monitor the proline ring puckering by means of simple 1D NMR spectroscopy.
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