Synthesis and characterization of composite metal complexes aiming at regulations of genetic information

• Project/Area Number: 21550070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Inorganic chemistry
• Research Institution: Chuo University
• Principal Investigator: CHIKIRA Makoto 中央大学, 理工学部, 教授 (70006328)
• Co-Investigator(Kenkyū-buntansha): KITAMURA Yusuke 中央大学, 理工学部, 助教 (80433019)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: DNA / 複合金属錯体 / 多核錯体 / DNAコンジュゲート / 抗癌活性 / 一塩基多形 / 複合化金属錯体 / 二核錯体 / カチオン性シッフ塩基錯体 / フェナントロリン / プロテインスプライシング反応

Research Abstract

We have synthesized various mixed ligand and multinuclear metal complexes and analyzed the DNA binding structures、evaluating the DNA binding affinity and the cleavage activity. The results indicated that the composite metal complexes strengthened efficiently the interaction with DNA and enhanced the cleavage activity. In addition、some of the complexes have been found to show a new type of anticancer activity. We have also synthesized new DNA metal complex conjugates and succeeded in developing an efficient technique for the detection of DNA SNPs by making use of protein splicing reactions.

Research Products

• [Journal Article] DNA molecular recognition and cellular selectivity of anticancer metal(II) complexes of ethylenediaminediacetate and phenanthroline : multiple targets (2012)
  • Author(s): Sze-Tin Von、Hoi-Ling Seng、Hong-Boon Lee、Seik-Weng Ng、Yusuke Kitamura、Makoto Chikira、Chew-Hee Ng
  • Journal Title: J. Inorg. Biol. Chem. Volume: Vol.17 No.1 Pages: 57-69
  • Peer Reviewed
• [Journal Article] DNA molecular recognition and cellular selectivity of anticancer metal (II) complexes of ethylenediaminediacetate and phenanthroline : multiple targets (2012)
  • Author(s): Sze-Tin Von, Hoi-Ling Seng, Hong-Boon Lee, Seik-Weng Ng, Yusuke Kitamura, Makoto Chikira, Chew-Hee Ng
  • Journal Title: J.Inorg.Biol.Chem. Volume: 17 Issue: 1 Pages: 57-69
  • DOI: 10.1007/s00775-011-0829-0
  • Peer Reviewed
• [Journal Article] Conformational change of ternary copper(II) complexes of cationic Schiff-bases and N-heteroaromatic amines induced by intercalative binding to DNA Inorg (2011)
  • Author(s): Yusuke Iwasaki、Masato Kimura、Atsushi Yamada、Yuichiro Mutoh、Maiko Tateishi、Hidekazu Arii、Yusuke Kitamura、Makoto Chikira
  • Journal Title: Chem. Commun. Volume: Vol.14 Pages: 1461-1464
  • Peer Reviewed
• [Journal Article] Conformational change of ternary copper (II) complexes of cationlc Schiff-bases and N-heteroaromatic amines induced by intercalative binding to DNA (2011)
  • Author(s): Yusuke Iwasaki, Masato Kimura, Atsushi Yamada, Yuichiro Mutoh, Maiko Tateishi, Hidekazu Arii, Yusuke Kitamura, Makoto Chikira
  • Journal Title: Inorganic Chemistry Communications Volume: 14 Issue: 9 Pages: 1461-1464
  • DOI: 10.1016/j.inoche.2011.05.047
  • Peer Reviewed
• [Journal Article] Copper(II) complexes of methylated glycine derivatives : Effect of methyl substituent on their DNA binding and nucleolytic property (2009)
  • Author(s): Hoi Ling Seng、Kong Wai Tan、Mohd Jamil Maah、Wee Tee Tan、Hirokazu Hamada、Makoto Chikira、Chew Hee Ng
  • Journal Title: Polyhedron Volume: Vol.28, No.11 Pages: 2219-2227
  • Peer Reviewed
• [Journal Article] ルテニウム(II)およびイリジウム(III)錯体の電気化学発光によるDNA二重らせんの検出 (2009)
  • Author(s): 櫻田伸一
  • Journal Title: 中央大学理工学研究所 論文集 15巻(掲載決定(印刷中))
  • Peer Reviewed
• [Journal Article] Copper(II)complexes of methylated glycine derivatives : Effect of methyl substituent on their DNA binding and nucleolytic property (2009)
  • Author(s): Hoi Ling Seng
  • Journal Title: Polyhedron Vol.28 Pages: 2219-2227
  • Peer Reviewed
• [Presentation] DNA binding affinity and cleavage activity of dicopper(II) Schiff base complex with cationic substituents (2011)
  • Author(s): Yoshie Hara、Akiyoshi Asahina、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] Effect of asymmetric bridging group on the binding of cationic salen type Schiff base complexes of nickel(II) to DNA (2011)
  • Author(s): Yoko Taruno、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] Synthesis of N-methylpyridinium Schiff base copper(II) complexes and evaluation of the interaction with DNA (2011)
  • Author(s): Shota Takakita、Masanobu Miyoshi、Akiyoshi Asahina、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] Synthesis of the DNA conjugates with metal chelator through cysteine moiety and the application to SNPs analysis (2011)
  • Author(s): Yuji Sakurai、Atsushi Nakano、Ayami Nogami、Toshihiro Ihara、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] Interaction of Salicylidene Amino Acid Schiff-base Oxovanadium(IV) Complexes with DNA (2011)
  • Author(s): Kazuya Takahashi、Asami Sano、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] DNA cleavage activity of dipicolylamine copper(II) complex tethered to Schiff base copper(II) complex (2011)
  • Author(s): Akiyosi Asahina、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] Effect of cationic side chains on the DNA binding and cleavage activity of Schiff base Copper(II) complexes having heterocyclic ligands (2011)
  • Author(s): Masato Kimura、Yusuke Iwasaki、Yusuke Kitamura、Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: New Delhi、India
  • Year and Date: 2011-10-19
• [Presentation] DNA binding affinity and cleavage activity of dicopper (II) Schiff base complex with cationic substituents (2011)
  • Author(s): Yoshie Hara, Akiyoshi Asahina, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-19
• [Presentation] Synthesis of N-methylpyridinium Schiff base copper (II) complexes an evaluation of the interaction with DNA (2011)
  • Author(s): Shota Takakita, Masanobu Miyoshi, Akiyoshi Asahina, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-17
• [Presentation] Effect of asymmetric bridging group on the binding of cationic salen type Schiff base complexes of nickel (II) to DNA (2011)
  • Author(s): Yoko Taruno, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-17
• [Presentation] Synthesis of the DNA conjugates with metal chelator through cysteine moiety and the application to SNPs analysis (2011)
  • Author(s): Yuji Sakurai, Atsushi Nakano, Ayami Nogami, Toshihiro Ihara, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-17
• [Presentation] DNA cleavage activity of dipicolylamine copper (II) complex tethered to Schiff base copper (II) complex (2011)
  • Author(s): Akiyosi Asahina, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-17
• [Presentation] Effect of cationic side chains on the DNA binding and cleavage activity of Schiff base Copper (II) complexes having heterocyclic ligands (2011)
  • Author(s): Masato Kimura, Yusuke Iwasaki, Yusuke Kitamura, Makoto Chikira
  • Organizer: 3rd Asian Conference on Coordination Chemistry
  • Place of Presentation: India Habitat Centre, New Delhi, India
  • Year and Date: 2011-10-17
• [Presentation] 新規カチオン性シッフ塩基銅(II)二核錯体のDNA結合親和性及び切断活性の評価 (2011)
  • Author(s): 原佳恵, 朝日向晃良, 北村裕介, 千喜良誠
  • Organizer: 第61回錯体化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2011-09-18
• [Presentation] 架橋部位に不斉を有するカチオン性サレン型シッフ塩基ニッケル(II)錯体とDNAとの結合構造解析 (2011)
  • Author(s): 埀野陽子・北村裕介・千喜良誠
  • Organizer: 第61回錯体化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2011-09-17
• [Presentation] N-メチルピリジニウムシッフ塩基銅(II)錯体の合成とDNAとの相互作用の評価 (2011)
  • Author(s): 高北翔大・三好正宣・朝日向晃良・北村裕介・千喜良誠
  • Organizer: 第61回錯体化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2011-09-17
• [Presentation] ジピコリルアミン銅(II)錯体を連結したシッフ塩基銅(II)三核錯体の合成とDNA切断活性の評価 (2011)
  • Author(s): 朝日向晃良・北村裕介・千喜良誠
  • Organizer: 第61回錯体化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2011-09-17
• [Presentation] 鋳型特異的にルテニウム-白金二核錯体を脱離する新規DNAブロープの設計とその遺伝子解析への応用 (2011)
  • Author(s): 戸田健太郎、富森岳、三田聡司、北村裕介、千喜良誠
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学・東山ホール
  • Year and Date: 2011-09-15
• [Presentation] リンカー部位にシステインを有する金属配位性DNAコンジュゲートの合成とその一塩基多型解析への応用 (2011)
  • Author(s): 櫻井悠司、中野篤、野上礼美、井原敏博、北村裕介、千喜良誠
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学・東山ホール
  • Year and Date: 2011-09-15
• [Presentation] Development of noble gene probes based on DNA-templated formation of platinum-ruthenium dinuclear complex (2010)
  • Author(s): Yusuke Kitamura、Takeshi Tomimori、Satoshi Mita、Makoto Chikira
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2010-12-17
• [Presentation] Template-directed formation of luminescent lanthanide complex using the DNA probes and application to gene analysis (2010)
  • Author(s): Nobuhiko Kaihara、Yusuke Kitamura、Takeshi Tomimori、Toshihiro Ihara、Makoto Chikira
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2010-12-17
• [Presentation] Template-directed formation of luminescent lanthanide complex using the DNA probes and application to gene analysis (2010)
  • Author(s): N.Kaihara
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Convention Center (Honolulu)
  • Year and Date: 2010-12-17
• [Presentation] DNA binding structure of zinc(II) and platinum(II) complexes with aromatic amine and edda (2010)
  • Author(s): Yusuke Nakamura、Hoi-Ling Seng、Chew-Hee Ng、Makoto Chikira
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2010-12-16
• [Presentation] Comparative study of the interactions of Schiffbase-N-heteroaromatic amine ternary complexes of Cr(III) and Cu(II) with DNA (2010)
  • Author(s): Shota Kitajima、Masato Kimura、Yusuke Kitamura、Makoto Chikira
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2010-12-16
• [Presentation] The synthesis、chHaracterization and DNA modification of new dicopper Schiff-base complexes (2010)
  • Author(s): Jian Shi、Midori Tojo、Yusuke Kitamura、Makoto Chikira
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2010-12-16
• [Presentation] The synthesis, characterization and DNA modification of new dicopper Schiff-base complexes (2010)
  • Author(s): J.Shi
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Convention Center (Honolulu)
  • Year and Date: 2010-12-16
• [Presentation] DNA binding structure of zinc(II) and platinum(II) complexes with aromatic amine and edda (2010)
  • Author(s): Y.Nakamura
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Convention Center (Honolulu)
  • Year and Date: 2010-12-16
• [Presentation] Comparative study of the interactions of Schiff-base-N-heteroaromatic amine ternary complexes of Cr(III) and Cu(II) with DNA (2010)
  • Author(s): S.Kitajima
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Convention Center (Honolulu)
  • Year and Date: 2010-12-16
• [Presentation] シッフ塩基銅錯体を修飾した新規銅三核錯体の合成とDNA切断活性の評価 (2010)
  • Author(s): 朝日向晃良
  • Organizer: 第60回錯体化学討論会
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-09-30
• [Presentation] Effect of cationic side chains on the interaction between DNA and amino acid Schiff base copper(II) complexes having heterocyclic ligands (2010)
  • Author(s): Masato Kimura、Yusuke Iwasaki、Yusuke Kitamura、Makoto Chikira
  • Organizer: 60th Anniversary Conference on Coordination Chemistry in OSAKA、JAPAN
  • Place of Presentation: Osaka、Japan
  • Year and Date: 2010-09-27
• [Presentation] Interaction of salicylideneamino acid Schiff base oxovanadium(IV) complexes with DNA (2010)
  • Author(s): Kazuya Takahashi、Asami Sano、Yusuke Kitamura、Makoto Chikira
  • Organizer: 60th Anniversary Conference on Coordination Chemistry in OSAKA、Japan
  • Place of Presentation: Osaka、Japan
  • Year and Date: 2010-09-27
• [Presentation] Effect of cationic side chains on the interaction between DNA and amino acid Schiff base copper(II) complexes having heterocyclic ligands (2010)
  • Author(s): M.Kimura
  • Organizer: 60th Anniversary Conference on Coordination Chemistry in OSAKA, JAPAN
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-09-26
• [Presentation] Interaction of salicylideneamino acid Schiff-base oxovanadium(IV) complexes with DNA (2010)
  • Author(s): K.Takahashi
  • Organizer: 60th Anniversary Conference on Coordination Chemistry in OSAKA, JAPAN
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-09-26
• [Presentation] Synthesis of new Ni(II) complexes of and evaluation of base recognition and binding affinity with DNA (2010)
  • Author(s): M.Miyoshi
  • Organizer: 60th Anniversary Conference on Coordination Chemistry in OSAKA, JAPAN
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-09-26
• [Presentation] 発光性希土類金属錯体を協同的に形成する新規核酸プローブの設計とその遺伝子解析への応用 (2010)
  • Author(s): 海原喜彦
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府)
  • Year and Date: 2010-09-25
• [Presentation] 鋳型特異的にルテニウム錯体を脱離するDNAプローブの設計とその遺伝子解析への応用 (2010)
  • Author(s): 戸田健太郎
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府)
  • Year and Date: 2010-09-25
• [Presentation] Interaction of Dinuclear Cationic Schiff Base Metal Complexes with DNA (2009)
  • Author(s): Makoto Chikira
  • Organizer: The Fourth Symposium on Advanced in Biological Inorganic Chemistry
  • Place of Presentation: TIFR(Mumbai, India)
  • Year and Date: 2009-11-05
• [Presentation] Nucleolytic Properties of Dinuclear Cationic Schiff Base Cu(II)and Ni(II)Complexes (2009)
  • Author(s): Makoto Chikira
  • Organizer: 16th International SPACC Symposium
  • Place of Presentation: Liaocheng Univ.(China)
  • Year and Date: 2009-10-29
• [Presentation] The Evaluation of DNA Base Sequence Recognition by Ternary Platinum(II)Complex with 1, 10-Phenanthroline and L-Arginine (2009)
  • Author(s): Hyejin Song
  • Organizer: 16th International SPACC Symposium
  • Place of Presentation: Liaocheng Univ.(China)
  • Year and Date: 2009-10-29
• [Presentation] Computer chemistry analysis of base recognition and structural fluctuation of oligonucleotides with Salen-type Ni(II)complex (2009)
  • Author(s): 杉本匡
  • Organizer: 16th International SPACC Symposium
  • Place of Presentation: Liaocheng Univ.(China)
  • Year and Date: 2009-10-29
• [Presentation] 鋳型特異的ルテニウム-白金二核錯体の形成とその遺伝子解析への応用 (2009)
  • Author(s): 北村裕介
  • Organizer: 第59回錯体化学討論会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2009-09-26
• [Presentation] 芳香族アミンと edda を有する亜鉛及び白金(II)錯体の合成とDNAとの結合構造解析 (2009)
  • Author(s): 中村雄祐
  • Organizer: 第59回錯体化学討論会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2009-09-25
• [Presentation] L-アルギニンを有する芳香族アミン三元系白金(II)錯体によるDNA塩基配列認識の評価 (2009)
  • Author(s): 宋慧珍
  • Organizer: 第59回錯体化学討論会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2009-09-25
• [Presentation] 計算化学によるオリゴヌクレオチドの構造揺らぎと遷移金属錯体の塩基認識性の解析 (2009)
  • Author(s): 杉本匡
  • Organizer: 第59回錯体化学討論会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2009-09-25
• [Presentation] 鋳型特異的に銅二核錯体を形成する新規DNAコンジユゲートの開発 (2009)
  • Author(s): 村田逸人
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2009-09-14
• [Presentation] DNAコンジュゲートによる発光性希土類金属錯体の協同的形成とその遺伝子解析への応用 (2009)
  • Author(s): 野上礼美
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2009-09-14
• [Presentation] 白金-ルテニウム二核錯体を鋳型特異的に形成する新規核酸プローブの設計とその遺伝子解析への応用 (2009)
  • Author(s): 富森岳
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2009-09-14
• [Presentation] 発光性希土類金属錯体を鋳型特異的に形成する新規核酸プローブの設計とその遺伝子解析への応用 (2009)
  • Author(s): 海原喜彦
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2009-09-14
• [Presentation] Interaction of Heterodinuclear Cationic Schiff Base Metal Complex with DNA (2009)
  • Author(s): Makoto Chikira
  • Organizer: 14^<th> International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Nagoya Congress Center,(Nagoya, Japan)
  • Year and Date: 2009-07-26
• [Remarks]
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  • URL: http://www.chem.chuo-u.ac.jp/~lbcc/publication.html
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  • URL: http://www.chem.chuo-u.ac.jp/~lbcc/publication.html
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  • URL: http://www.chem.chuo-u.ac.jp/~lbcc/publication.html