| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
This research aimed at developing a novel method for measurement of oxidative stress in a single cell. Firstly, behavior of a single cell before and after an oxidative stress such as by addition of a hydrogen peroxide solution was monitored and compared by the deflection method of a probe beam. Then, analysis of active oxygen species in cells was carried out by a home-made capillary electrophoresis-laser induced fluorescence detection system. The luminol-chemliuminescence method was further used for quantitative analysis of active oxygen species in cells. HeG2 cell was cultured and used as a model cell sample.
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