Measurement of Oxidative Stress on a single cell level by the beam deflection method and capillary electrophoresis
Project/Area Number |
21550076
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | Fukuoka Institute of Technology (2010-2011) University of Fukui (2009) |
Principal Investigator |
WU Xing-zheng 福岡工業大学, 工学部, 教授 (70234961)
|
Co-Investigator(Kenkyū-buntansha) |
TERADA Satoshi 福井大学, 大学院・工学研究科, 准教授 (60311685)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 計測 / 細胞 / 酸化ストレス / 活性酸素 / プローブ光の偏向 / 電気泳動 / 化学発光 |
Research Abstract |
This research aimed at developing a novel method for measurement of oxidative stress in a single cell. Firstly, behavior of a single cell before and after an oxidative stress such as by addition of a hydrogen peroxide solution was monitored and compared by the deflection method of a probe beam. Then, analysis of active oxygen species in cells was carried out by a home-made capillary electrophoresis-laser induced fluorescence detection system. The luminol-chemliuminescence method was further used for quantitative analysis of active oxygen species in cells. HeG2 cell was cultured and used as a model cell sample.
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Report
(4 results)
Research Products
(26 results)