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New analytical method on channel transports across bilayer lipid membranes

Research Project

Project/Area Number 21550079
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionKyoto University

Principal Investigator

SHIRAI Osamu  京都大学, 大学院・農学研究科, 准教授 (40355011)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords状態分析 / イオンチャネル / イオン透過 / 脂質二分子膜 / KAT1チャネル / アンホテリシンB / 電気的中性則 / 電気化学測定 / リポソーム / グラミシジンA / KAT 1チャネル
Research Abstract

New analytical method on channel transports across bilayer lipid membranes was proposed by considering mass balance and electroneutrality. When the ion channel serves in the membrane, not only the transport of the target ion but also those of coexsisting ions should be evaluated. The ion-transport property was not controlled by only the relation between the ion size and the pore-size of the ion channel. It was proved that the distribution coefficients of both the target ion and the counter ion and their diffusion coefficients within the membrane were important factors.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (55 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (39 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Electrochemical Investigation on Permeability of Organic Acid Ions Through amphotericin B channels2012

    • Author(s)
      Takafumi Yamauchi, Osamu Shirai, Seiya Tsujimura, and Kenji Kano
    • Journal Title

      Electrochemistry

      Volume: 80(5) Pages: 315-317

    • NAID

      10030143724

    • URL

      http://dx.doi.org/10.5796/electrochemistry/80.315

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of Inhalation Anesthetics on Ion Transport across a Planar Bilayer Lipid Membrane2012

    • Author(s)
      Kei Hichiri, Osamu Shirai, and Kenji Kano
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 1 Pages: 45

    • DOI

      10.2116/analsci.28.45

    • NAID

      130004827176

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Investigation on Permeability of Organic Acid Ions Through amphotericin B channels2012

    • Author(s)
      Takafumi Yamauchi, Osamu Shirai, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Electrochemistry

      Volume: (in press)(印刷中のため未確認)

    • NAID

      10030143724

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ion Transport across Planar Bilayer Lipid Membrane Driven by D-Fructose Dehydrogenase-Catalyzed Electron Transport2011

    • Author(s)
      Keisuke Sasakura, Osamu Shirai, Kei Hichiri, Maiko Goda-Tsutsumi, Seiya Tsujimura, and Kenji Kano
    • Journal Title

      Chemistry Letters

      Volume: 40 Issue: 5 Pages: 486-488

    • DOI

      10.1246/cl.2011.486

    • NAID

      130004426208

    • Related Report
      2011 Annual Research Report 2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical elucidation of the facilitated ion transport across a bilayer lipid membrane in the presence of neutral carrier compounds2010

    • Author(s)
      Jun Onishi, Osamu Shirai and Kenji Kano
    • Journal Title

      Electroanalysis

      Volume: 22(11) Issue: 11 Pages: 1229-1238

    • DOI

      10.1002/elan.200900481

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioelectrocatalytic Endpoint Assays Based on Steady-state Diffusion Current at Microelectrode Array2010

    • Author(s)
      Tatsuo Nods, Katsumi Hamamoto, K., Maiko Goda-Tsutsumi, Seiya Tsujimura, Osamu Shirai, Kenji Kano
    • Journal Title

      Electrochemistry Communications

      Volume: 12(8) Pages: 839-842

    • NAID

      120002511362

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical elucidation of the facilitated ion transport across a bilayer lipid membrane in the presence of neutral carrier compounds2010

    • Author(s)
      J.Onishi, O.Shirai, K.Kano
    • Journal Title

      Electroanalysis (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 分極曲線・サイクリックボルタンメトリー(5)生物電気化2009

    • Author(s)
      辻村清也, 白井理
    • Journal Title

      electrochemistry

      Volume: 77 Pages: 486-492

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Osamu Shirai, Shunsuke Ozaki, Jun Onishi, N. Kozai, Toshihiko Ohnuki and Kenji Kano
    • Journal Title

      Chemistry Letters

      Volume: 38 Issue: 11 Pages: 1038-1039

    • DOI

      10.1246/cl.2009.1038

    • NAID

      10025547780

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ion transport across a bilayer lipid membrane in the presence of hydrophobic ions2009

    • Author(s)
      O.Shirai, S.Ozaki, J.Onishi, N.Kozai, T.Ohnuki, K.Kano
    • Journal Title

      Chemistry Letters 38

      Pages: 1038-1039

    • NAID

      10025547780

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 分極曲線・サイクリックボルタンメトリー(5)生物電気化学2009

    • Author(s)
      辻村清也、白井理
    • Journal Title

      Electrochemistry 77

      Pages: 486-492

    • NAID

      10024980169

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 脂質二分子膜を介した電子移動駆動型イオン輸送2012

    • Author(s)
      七里慧, 白井理, 加納健司
    • Organizer
      電気化学会第79回大会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] モデル生体膜系における膜結合型酵素D-Fructose Dehydrogenaseによる電子移動に駆動されたイオン透過2012

    • Author(s)
      七里慧, 白井理, 加納健司
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都市
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] モデル生体膜系における膜結合型酵素D-Fructose Dehydrogenaseによる電子移動に駆動されたイオン透過2012

    • Author(s)
      七里慧, 白井理, 加納健司
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜電位差の伝播機構の基礎検討2011

    • Author(s)
      植屋宣義, 串田優輝, 白井理, 加納健司
    • Organizer
      第57回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      那覇市
    • Year and Date
      2011-12-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 膜電位差の伝播機構の基礎検討2011

    • Author(s)
      植屋宣義, 串田優輝, 白井理, 加納健司
    • Organizer
      第57回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      沖縄県男女共同参画センター「てぃるる」
    • Year and Date
      2011-12-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Selective transport of organic acids across bilayer lipid membranes in the presence of amphotericin B channels2011

    • Author(s)
      山内隆史, 松本隆平, 藤田修二, 酒井秀樹, 渡辺陽子, 戸木田陽一, 辻村清也, 白井理, 加納健司
    • Organizer
      Shikata Discussion 2011
    • Place of Presentation
      Awaji
    • Year and Date
      2011-05-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Selective Transport of Organic Acids across Bilayer Lipid Membranes in the Presence of Amphotericin B Channels2011

    • Author(s)
      Takafumi Yamauchi, Ryuhei Matsumoto, Shuji Fujita, Hideki Sakai, Yoko Watanabe, Yuichi Tokita, Seiya Tsujimura, Osamu Shirai, Kenji Kano
    • Organizer
      Shikata Discussion 2011
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Hyogo, Japan
    • Year and Date
      2011-05-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Neurotransmission model by use of liquid membrane cell system2011

    • Author(s)
      植屋宣義, 白井理, 辻村清也, 加納健司
    • Organizer
      International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto
    • Year and Date
      2011-05-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Neurotransmission Model by Use of Liquid Membrane Cell System2011

    • Author(s)
      Noriyoshi Ueya, Osamu Shirai, Seiya Tsujimura, Kenji Kano
    • Organizer
      International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2011-05-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 機液膜型セルを用いた神経伝達メカニズムの新規提案2011

    • Author(s)
      植屋宣義, 白井理, 辻村清也, 加納健司
    • Organizer
      2011年電気化学会第78回大会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 有機液膜型セルを用いた神経伝達メカニズムの新規提案2011

    • Author(s)
      植屋宣義, 白井理, 辻村清也, 加納健司
    • Organizer
      2011年電気化学会第78回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 吸入麻酔剤の作用機序についての基礎検討2010

    • Author(s)
      白井理, 七里慧, 辻村清也, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田市
    • Year and Date
      2010-11-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体機能発現の場・生体膜2010

    • Author(s)
      白井理, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田市
    • Year and Date
      2010-11-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体機能発現の場・生体膜2010

    • Author(s)
      白井理, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田大学(招待講演)
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体機能発現の場・生体膜2010

    • Author(s)
      白井理, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 有機液膜系を利用した神経伝達メカニズムの新規提案2010

    • Author(s)
      植屋宣義, 白井理, 辻村清也, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 吸入麻酔剤の作用機序についての基礎検討2010

    • Author(s)
      白井理, 七里慧, 辻村清也, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 脂質二分子膜を介した物質輸送に与えるポリイオンの効果2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田市
    • Year and Date
      2010-11-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 脂質二分子膜を介した物質輸送に与えるポリイオンの効果2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 球形拡散に基づく電気化学バイオセンサの開発2010

    • Author(s)
      野田達夫, 鰐淵瑞絵, 濱本勝美, 辻村清也, 白井理, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] メタゲノム由来ラッカーゼの酵素電極触媒反応に与える表面特性の影響2010

    • Author(s)
      朝日将史, 堤真衣子, 辻村清也, 白井理, 宮崎健太郎, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      秋田大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体膜モデルにおける膜結合型酵素Fructose Dehydrogenase (FDH)による電子伝達機構の解明2010

    • Author(s)
      七里慧, 白井理, 辻村清也, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリイオンによる脂質二分子膜を介した促進イオン移動2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 液膜型セルを用いた膜電位伝播機構の解明-神経伝達モデルの構築-2010

    • Author(s)
      植屋宣義, 白井理, 辻村清也, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 電気化学的手法によるカリウムチャネルKAT1のイオン透過および阻害様式の解明2010

    • Author(s)
      窪田慎太郎, 白井理, 戸澤譲, 青木志保, 日び隆雄, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリイオンによる脂質二分子膜を介した促進イオン移動2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] アムホテリシンBチャネルによる脂質二分子膜を介した親水性分子の選択的透過2010

    • Author(s)
      山内隆史, 藤田修二, 松本隆平, 酒井秀樹, 戸木田裕一, 辻村清也, 白井理, 加納健司
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体膜モデルを介した物質輸送に及ぼすポリイオンの影響2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      2010年電気化学秋季大会
    • Place of Presentation
      厚木市
    • Year and Date
      2010-09-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体膜モデルを介した物質輸送に及ぼすポリイオンの影響2010

    • Author(s)
      白井理, 新村美智子, 辻村清也, 加納健司
    • Organizer
      2010年電気化学秋季大会
    • Place of Presentation
      神奈川工科大学
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ion Transport Facilitated by Carrier Compounds across a Bilayer Lipid Membrane2010

    • Author(s)
      白井理, 大西純, 加納健司
    • Organizer
      The 7th Asian Conferenve on Electrochemistry in Kumamoto
    • Place of Presentation
      熊本市
    • Year and Date
      2010-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Ion Transport Facilitated by Carrier Compounds across a Bilayer Lipid Membrane2010

    • Author(s)
      Osamu Shirai, Jun Onishi, Kenji Kano
    • Organizer
      The 7^<th> Asian Conference on Electrochemistry in Kumamoto
    • Place of Presentation
      熊本市(招待講演)
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 吸入麻酔剤の作用メカニズムの新解釈2010

    • Author(s)
      七里慧, 辻村清也, 白井理, 加納健司
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      松江市
    • Year and Date
      2010-05-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 吸入麻酔剤の作用メカニズムの新解釈2010

    • Author(s)
      七里慧, 辻村清也, 白井理, 加納健司
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      島根大学
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 有機液膜型セルを用いた神経伝導メカニズムの新解釈2010

    • Author(s)
      植屋宣義, 辻村清也, 白井理, 加納健司
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      島根大学
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 膜内メディエータと膜結合型酵素との電子移動反応2009

    • Author(s)
      笹倉啓資、白井理、加納健司
    • Organizer
      電気化学会
    • Place of Presentation
      東京農工大学(東京都)
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] グラミシジンAチャネル透過電流におけるイオンの分配と膜内拡散に基づく評価2009

    • Author(s)
      窪田慎太郎、白井理、加納健司
    • Organizer
      電気化学会
    • Place of Presentation
      東京農工大学(東京都)
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生体膜の機能評価2009

    • Author(s)
      白井理, 加納健司
    • Organizer
      2009年電気化学秋季大会
    • Place of Presentation
      小金井市
    • Year and Date
      2009-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体膜の機能評価2009

    • Author(s)
      白井理、加納健司
    • Organizer
      電気化学会
    • Place of Presentation
      東京農工大学(東京都)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 中性キャリアによる脂質二分子膜2009

    • Author(s)
      大西純、白井理、加納健司
    • Organizer
      日本分析化学会
    • Place of Presentation
      和歌山大学(和歌山県)
    • Year and Date
      2009-05-17
    • Related Report
      2009 Annual Research Report
  • [Book] バイオ電池の最新動向2011

    • Author(s)
      白井理
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] バイオ電池の最新動向2011

    • Author(s)
      白井理(分担執筆)
    • Total Pages
      5
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bapc.kais.kyoto-u.ac.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.bapc.kais.kyoto-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bapc.kais.kyoto-u.ac.jp/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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