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DNA-chip-based gene sensor based on in-situ electronical functionalization of double-helical DNA self-assemblies.

Research Project

Project/Area Number 21550085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionKyushu University

Principal Investigator

NAKANO Koji  九州大学, 工学研究院, 准教授 (10180324)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsナノバイオ / DNA / 自己組織化 / 分子認識 / バイオセンサー / 遺伝子センサー / 分子エレクトロニクス / 走査プローブ顕微鏡
Research Abstract

DNA-based molecular device tackled for futuristic gene sensor developmentsbased on in-situ electroconductivemodification of wasDNA self-assembly. Some direct or indirect assistance necessary to the goal were successfully developed ; novel small-molecule, reative compounds that enable versatile functionalization of DNA duplexes, scanning electrochemical microscopy imaging of DNA microdots, a new potentiometric platform for gene sensing, and etc. Unfortunately, the planned strategy, dip-pen nanolithography, has not yet achieved mostly due to the mechanical instability of the DNA self-assemblies. However, are committed to taking on the challengesbyusingsome DNAnanomaterialstoprovidetheparticulawergene-sensing device.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (53 results)

All 2012 2011 2010 2009 Other

All Journal Article (28 results) (of which Peer Reviewed: 25 results) Presentation (20 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Programmable protein-protein conjugation via DNA-based self-assembly2012

    • Author(s)
      J. Shimada, T. Maruyama, M. Kitaoka, H. Yoshinaga, K. Nakano, N. Kamiya, M. Goto
    • Journal Title

      Chemical Communications

      Volume: (in press)

    • NAID

      120006318790

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Programmable protein-protein conjugation via DNA-based self-assembly2012

    • Author(s)
      J.Shimada, et.al.
    • Journal Title

      Chemical Communications

      Volume: (in press) Issue: 50 Pages: 6226-6226

    • DOI

      10.1039/c2cc30618b

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A simple and selective fluorometric assay for dopamine using a calcein blue-Fe^<2+> complex fluorophore2012

    • Author(s)
      Daisuke Seto, Tomoharu Maki, Nobuaki Soh, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato
    • Journal Title

      Talanta

      Volume: 94 Pages: 36-43

    • DOI

      10.1016/j.talanta.2012.02.025

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform2012

    • Author(s)
      Shuai Guo
    • Journal Title

      Pure and Applied Chemistry

      Volume: (未定) Issue: 10 Pages: 2027-2043

    • DOI

      10.1351/pac-con-11-10-10

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA2012

    • Author(s)
      Mayo Miyake, et al
    • Journal Title

      Talanta

      Volume: (未定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-Reactivity Relationship of Piperidine Nitroxide : Electrochemical, ESR and Computational Studies2011

    • Author(s)
      T. Yamasaki, F. Mito, Y. Ito, S. Pandian, Y. Konoshita, K. Nakano, R. Murugesan, K. Sakai, H. Utsumi, K. Yamada
    • Journal Title

      J. Organic Chemistry

      Volume: Vol.76, No.2 Pages: 435-440

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 原子間力顕微鏡(AFM)で生体分子を見る2011

    • Author(s)
      中野幸二
    • Journal Title

      化学と工業

      Volume: 64巻6号 Pages: 460-461

    • NAID

      10028244559

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Structure-Reactivity Relationship of Piperidine Nitroxide : Electrochemical, ESR and Computational Studies2011

    • Author(s)
      Toshihide Yamasaki, et al
    • Journal Title

      J.Organic Chemistry

      Volume: 76 Issue: 2 Pages: 435-440

    • DOI

      10.1021/jo101961m

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anodic Stripping Voltammetry Combined with Sequential Injection Analysis for Measurements of Trace Metal Ions with Bismuth- and Antimony Film Electrodes under Comparable Conditions2011

    • Author(s)
      Valeria Guzsvany, et al
    • Journal Title

      Electroanalysis

      Volume: 23 Issue: 7 Pages: 1593-1601

    • DOI

      10.1002/elan.201000683

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flow injection fluorometric determination of ascorbic acid using perylene bisimide-linked nitroxide2011

    • Author(s)
      Tomoharu Maki, et al
    • Journal Title

      Talanta

      Volume: 85 Issue: 4 Pages: 1730-1733

    • DOI

      10.1016/j.talanta.2011.06.054

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An integrated enzyme-linked immunosorbent assay system with an organic light-emitting diode and a chargecoupled device for fluorescence detection2011

    • Author(s)
      Hizuru Nakajima, et al
    • Journal Title

      J Separation Science

      Volume: 34 Issue: 20 Pages: 2906-2912

    • DOI

      10.1002/jssc.201100429

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A surface plasmon resonance sensor on a compact disk-type microfluidic device2011

    • Author(s)
      Akihide Henmi, et al
    • Journal Title

      J Separation Science

      Volume: 34 Issue: 20 Pages: 2913-2919

    • DOI

      10.1002/jssc.201100446

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free DNA detection platform based on atomic force microscopy visualisation : characterising the molecular-recognition-triggered conformational changes of an immobilised receptor oligonucleotide probe2010

    • Author(s)
      K. Nakano, H. Yamanouchi, H. Yoshinaga, N. Soh, T. Imato
    • Journal Title

      Chemical Communications

      Volume: Vol.46, No.31 Pages: 5683-5685

    • NAID

      120005227162

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An atomic force microscopy assay of intercalation binding, unwinding and elongation of DNA, using a water-soluble psoralen derivative a covalent binding probe molecule2010

    • Author(s)
      K. Nakano, Y. Katsumi, N. Soh, T. Imato
    • Journal Title

      Bulleas tin of the Chemical Society of Japan

      Volume: Vol.83, No.3 Pages: 273-275

    • NAID

      10027172640

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-free DNA Detection Platform Based on Atomic Force Microscopy Visualisation : Characterising the Molecular-Recognition-Triggered Conformational Changes of an Immobilised Receptor Oligonucleotide Probe2010

    • Author(s)
      Koji Nakano, et al.
    • Journal Title

      Chemical Communications

      Volume: 46 Pages: 5683-5685

    • NAID

      120005227162

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Fluorescence Detection of Histamine Based on Ligand Exchange Mechanism and Its Application to Biomonitoring2010

    • Author(s)
      Daisuke Seto, et al.
    • Journal Title

      Analytical Biochemistry

      Volume: 404 Pages: 135-139

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An Amphiphilic Fluorescent Probe for the Visualization of Histamine in Living Cells2010

    • Author(s)
      Daisuke Seto, et al.
    • Journal Title

      Bioorganic Medical Chemistry Letters

      Volume: 20 Pages: 6708-6711

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antimony-Film Electrode for the Determination of Trace Metals by Sequential-Injection Analysis/Anodic Stripping Voltammetry2010

    • Author(s)
      Valeria GUZSVANY, et al.
    • Journal Title

      Analytica Chimica Acta 658

      Pages: 12-17

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An atomic force microscopy assay of intercalation binding, unwinding and elongation of DNA, using a water-soluble psoralen derivative as a covalent binding probe molecule2010

    • Author(s)
      中野幸二, ら
    • Journal Title

      Bulletin of the Chemical Society of Japan 83

      Pages: 273-275

    • NAID

      10027172640

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimuli-responsive nanoparticles composed of naturally occurring amphiphilic proteins2009

    • Author(s)
      N. Kamiya, Y. Shiotari, M. Tokunaga, H. Matsunaga, H. Yamanouchi, K. Nakano, M. Goto
    • Journal Title

      Chemical Communications

      Volume: No.35 Pages: 5287-5289

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence As Molecular-Physical Probe For Nucleic Acid Hybridization Detection Based On Atomic Force Microscopy Imaging2009

    • Author(s)
      K. Nakano, H. Matsunaga, M. Murata, N. Soh, T. Imato
    • Journal Title

      Analytical Sciences

      Volume: Vol.25, No.8 Pages: 993-998

    • NAID

      10025214005

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perylenebisimide-Linked Nitroxide for the Detection of Hydroxyl Radicals2009

    • Author(s)
      Tomoharu Maki, et al.
    • Journal Title

      Analytica Chimica Acta 639

      Pages: 78-82

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Positive-Feedback-Mode Scanning Electrochemical Microscopy Imaging of Redox-Active DNA-Poly(1,4-benzoquinone)Conjugate Film Deposited on Carbon Fiber Electrode for Micrometer-Sized Hybridization Biosensor Applications2009

    • Author(s)
      中野幸二, ら
    • Journal Title

      J.Electroanalytical Chemistry 628

      Pages: 113-118

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence as Molecular-Physical Probe for Nucleic Acid Hybridization Detection Based on Atomic Force Microscopy Imaging2009

    • Author(s)
      中野幸二, ら
    • Journal Title

      Analytical Sciences 25

      Pages: 993-998

    • NAID

      10025214005

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flow Immunoassay for Nonioinic Surfactants Based on Surface Plasmon Resonance Sensors2009

    • Author(s)
      Mayumi TANAKA, et al.
    • Journal Title

      Analytical Sciences 25

      Pages: 999-1005

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimuli-Responsive Nanoparticles Composed of Naturally Occurring Amphiphilic Proteins2009

    • Author(s)
      Noriho KAMIYA, et al.
    • Journal Title

      Chemical Communications 35

      Pages: 5287-5289

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アルカンチオール単分子膜修飾電極に固定化したチトクロームcの直接電子移動反応の研究2009

    • Author(s)
      中野幸二, ら
    • Journal Title

      九州大学中央分析センター報告 27

      Pages: 17-24

    • NAID

      40016978896

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      Valeria GUZSVANY, et al.
    • Journal Title

      Sensing in Electroanalysis Volume 4(K.Vytras, K.Kalcher, I.Svancara, Eds.)(University of Pardubice, Pardubice, Czech Republic)

      Pages: 121-132

    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA Dendrimer Self-Assembly for Nano-Biosensing Applications2011

    • Author(s)
      Koji Nakano
    • Organizer
      The 2011 Global COE International Symposium on Future Molecular Systems
    • Place of Presentation
      福岡市(invited)
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホスホロチオェステル化オリゴヌクレオチドからのピボット型自己組織化膜形成とDNA修飾電極への応用2011

    • Author(s)
      吉永尚生, ら
    • Organizer
      分析化学会第60年会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 酸化還元ラベル化DNA対を用いる電位測定型遺伝子センシング2011

    • Author(s)
      中野幸二, ら
    • Organizer
      分析化学会第60年会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光架橋反応と組み合わせた自己組織化DNAデンドリマー形成と化学増幅型DNAマスセンシングへの応用2011

    • Author(s)
      澤田貴文, ら
    • Organizer
      分析化学会第60年会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Self-Assembly of Dendrimer-Like DNA and Electrical Conductivity Measurements Using Interdigitated Microelectrodes2011

    • Author(s)
      Koji Nakano
    • Organizer
      International colloquium on "Recent progress in nanofabrications of MEMS and NEMS : Science and innovation technologies"
    • Place of Presentation
      福岡市
    • Year and Date
      2011-03-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Potentiometric Gene Sensor Using Redox-Labeled Oligonucleotide Probe Pair2011

    • Author(s)
      Koji Nakano, et al.
    • Organizer
      International colloquium on "Recent progress in nanofabrications of MEMS and NEMS : Science and innovation technologies"
    • Place of Presentation
      福岡市
    • Year and Date
      2011-03-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA Dendrimer Self-Assembly for Nano-Biosensing Applications2011

    • Author(s)
      K. Nakano
    • Organizer
      The 2011 Global COE International Symposium on Future Molecular Systems
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Positive-Feedback-Mode Scanning Electrochemical Microscopy Imaging of DNA-Grafted Benzoquinone Polymer Cast Film for Micrometer-Sized Hybridization Biosensor Applications2010

    • Author(s)
      Koji Nakano, et al.
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phosphorothioate DNA Self-Assembly Developed on Gold Substrates as Characterized by IR, Ellipsometry, Microgravimetric Analysis, and AFM for Application to SNPs Bioassay Taking Advantage of Supramolecular Conformation Transitions2010

    • Author(s)
      Hisao Yoshinaga, et al.
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] AFM Visualization Read-Out od DNA Molecular Recognition for Green Bioanalysis Platform with Non-Label, Single-Molecule Detection Capability2010

    • Author(s)
      Koji Nakano, et al.
    • Organizer
      Kumoh National Institute of Technology-Kyushu University Joint Workshop on Green Energy
    • Place of Presentation
      Kumoh, Korea
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Potentiometric Gene Sensor Based on EMF Generation from Redox-Labelled Oligonucleotide Probe Pair2010

    • Author(s)
      Koji Nakano, et al.
    • Organizer
      Faraday Discussion 149 : Analysis for Healthcare Diagnostics and Theranostics
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA and DNA Conjugate Self-Assembly Developed on Au(111)Substrate as Characterized by Conductive Atomic Force Microscopy2010

    • Author(s)
      Koji NAKANO
    • Organizer
      International colloquium on "Recent progress in nanotechnologies : From nanoindentation on semiconductor materials to DNA and molecular devices"
    • Place of Presentation
      福岡市
    • Year and Date
      2010-03-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Positive-feedback-mode scanning electrochemical microscopy imaging of DNA-grafted benzoquinone polymer cast film for micrometer-sized hybridization biosensor applications2010

    • Author(s)
      K. Nakano, K. Nakamura, T. Kimura, H. Yoshinaga, N. Soh, T. Imato
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Potentiometric Gene Sensor based on EMF Generation from Redox-Labelled Oligonucleotide Probe Pair2010

    • Author(s)
      K. Nakano, T. Kimura, H. Yoshinaga N. Soh, T. Imato
    • Organizer
      Faraday Discussion 149 : Analysis for Healthcare Diagnostics and Theranostics
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2011 Final Research Report
  • [Presentation] 光架橋性付着末端を持つ三叉構造オリゴヌクレオチドモチーフからのデンドリマー生成とナノ会合体形成を利用するDNAの化学計測法2009

    • Author(s)
      森美詞, ら
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペリレンを主骨格としたヒドロキシラジカル計測用蛍光プローブの開発2009

    • Author(s)
      牧知治, ら
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Atomic Force Microscopy Imaging of Stem-Loop to Double-Helical Superstructure Transition of Probe DNA Sequences and Application to Single-Nucleotide Polymorphism Assay2009

    • Author(s)
      Hisao YOSHINAGA, et al.
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 金属キレートの配位子置換反応を利用するヒスタミン検出用蛍光プローブの開発2009

    • Author(s)
      瀬戸大輔, ら
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 有機ELと有機薄膜フォトダイオードをオンチップ化したマイクロ光検出システムの開発2009

    • Author(s)
      三宅麻代, ら
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] バイオ分析応用を目指した発光性微粒子の開発2009

    • Author(s)
      今里健太, ら
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Book] Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications, Modern Aspects of Electrochemistry. Applications of Electrochemistry and Nanotechnology in Biology and Medicine II2011

    • Author(s)
      K. Nakano
    • Publisher
      Springer, New York
    • Related Report
      2011 Final Research Report
  • [Book] Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications, in Modern Aspects of Electrochemistry 53, Applications of Electrochemistry and Nanotechnology in Biology and Medicine II, Ed by Noam Eliaz2011

    • Author(s)
      Koji Nakano
    • Total Pages
      338
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Book] Faraday Discussions Vol 149Analysis for Healthcare Diagnostics and Theranostics(P.Earis Ed)2011

    • Author(s)
      Koji Nakano
    • Total Pages
      370
    • Publisher
      RSC Publishing
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.cstf.kyushu-u.ac.jp/~imatolab/index-j.html

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] Potentiometric Gene Sensor Based on EMF Generation from a Redox-Labeled Oligonucleotide Probe Pair2010

    • Inventor(s)
      K. Nakano, T. Kimura, T. Imato
    • Industrial Property Rights Holder
      K. Nakano, T. Kimura, T. Imato
    • Filing Date
      2010-08-23
    • Related Report
      2011 Final Research Report
    • Overseas

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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