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Method Development for Assessing the Progression of Metabolic Syndrome by Multiplex-dynamic Analysis of Compounds of Biological Importance

Research Project

Project/Area Number 21550089
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

KUSU Fumiyo  東京薬科大学, 薬学部, 教授 (70057371)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsメタボリックシンドローム / コレステロール / 遊離脂肪酸 / 電気化学分析 / 分析科学 / 薬学 / 動脈硬化 / 糖尿病 / 血糖値 / イソフラボン / コレスタノール / 酸化ステロール / 心筋梗塞 / 低比重リポタンパク質
Research Abstract

The multiplex analytical methods for determining compounds of biological importance were developed to examine the progression of metabolic syndrome. Dynamic analyses of plasma free fatty acids, cholesterol, and pathologically important sterols in animal model of disease were performed by sensitive and precise HPLC with electrochemical detection. Moreover, a novel analytical strategy based on statistics aspect was shown to be applicable to examine the interaction between free fatty acids and blood glucose level. It was concluded that the present methods were useful various kinetic studies in clinical fields.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (29 results)

All 2011 2010 2009

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (14 results)

  • [Journal Article] Simultaneous determination of serum lathosterol and cholesterol by semi-micro high-performance liquid chromatography with electrochemical detection2011

    • Author(s)
      K. Hojo, H. Hakamata, F. Kusu
    • Journal Title

      J. Chromatogr. B

      Volume: 879 Issue: 11-12 Pages: 751-755

    • DOI

      10.1016/j.jchromb.2011.02.017

    • NAID

      10029518608

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of oxysterols in oxidatively modified low density lipoprotein by MALDI-TOF MS2011

    • Author(s)
      M. Hasegawa, H. Hakamata, I. Matsunaga, F. Kusu
    • Journal Title

      Eur. J. Lipid Sci. Technol.

      Volume: 113 Issue: 4 Pages: 423-429

    • DOI

      10.1002/ejlt.201000366

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Picomole level determination of cholesterol by HPLC with electrochemical detection using boron-doped diamond electrode after performance assessment based on the FUMI theory2011

    • Author(s)
      A. Kotani, H. Hakamata, N. Nakayama, F. Kusu
    • Journal Title

      Electroanalysis

      Volume: 23 Issue: 11 Pages: 2709-2715

    • DOI

      10.1002/elan.201100223

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of serum cholestanol by semi-micro high-performance liquid chromatography with electrochemical detection2010

    • Author(s)
      K. Hojo, H. Hakamata, A. Takahashi, Y. Y. Hosokawa, F. Kusu
    • Journal Title

      Biomed. Chromatogr.

      Volume: 24 Issue: 6 Pages: 600-605

    • DOI

      10.1002/bmc.1332

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of β-sitosterol, campesterol, stigmasterol, and brassicasterol in serum by high-performance liquid chromatography with electrochemical detection2010

    • Author(s)
      N. Itoh, H. Hakamata, F. Kusu
    • Journal Title

      Anal. Methods

      Volume: 2 Issue: 2 Pages: 174-179

    • DOI

      10.1039/b9ay00195f

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Column-switching high-performance liquid chromatography with two channels electrochemical detection for high-sensitive determination of isoflavones2010

    • Author(s)
      D. Matsumoto, A. Kotani, H. Hakamata, K. Takahashi, F. Kusu
    • Journal Title

      J. Chromatogr. A

      Volume: 1217 Issue: 17 Pages: 2986-2989

    • DOI

      10.1016/j.chroma.2010.02.050

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of serum cholestanol by semi-micro high-performance liquid chromatography with electrochemical detection2010

    • Author(s)
      K.Hojo, H.Hakamata, A.Takahashi, Y.Y Hosokawa, F.Kusu.
    • Journal Title

      Biomed.Chromatogr.

      Volume: 24 Pages: 600-605

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of β-sitosterol, campesterol, stigmasterol, and brassicasterol in serum by high-performance liquid chromatography with electrochemical detection2010

    • Author(s)
      N.Itoh, H.Hakamata, F.Kusu
    • Journal Title

      Anal.Method.

      Volume: 2 Pages: 174-479

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Column-switching high-performance liquid chromatography with two channels electrochemical detection for high-sensitive determination of isoflavones2010

    • Author(s)
      D.Matsumoto, A.Kotani, H.Hakamata, K.Takahashi, F.Kusu
    • Journal Title

      J.Chromatogr.A

      Volume: 1217 Pages: 2986-2989

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of oxysterols in oxidatively modified low-density lipoprotein by semi-micro high-performance liquid chromatography with electrochemical detection2009

    • Author(s)
      I. Matsunaga, H. Hakamata, K. Sadohara, K. Kakiuchi, F. Kusu
    • Journal Title

      Anal. Biochem.

      Volume: 393 Issue: 2 Pages: 222-228

    • DOI

      10.1016/j.ab.2009.06.032

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An LC method for monitoring medium-chain fatty acid permeation through CaCo-2 cell monolayers2009

    • Author(s)
      K. Yamamoto, H. Hakamata, A. Yamaguchi, F. Kusu
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 395 Pages: 533-538

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection2009

    • Author(s)
      A. Kotani, Y. Miyaguchi, M. Kohama, T. Ohtsuka, T. Shiratori, F. Kusu
    • Journal Title

      Analytical Sciences

      Volume: 25 Issue: 8 Pages: 1007-1011

    • DOI

      10.2116/analsci.25.1007

    • NAID

      10025214039

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of oxysterols in oxidatively modified low-density lipoprotein by semi-micro high-performance liquid chromatography with electrochemical detection2009

    • Author(s)
      I.Matsunaga, H.Hakamata, K.Sadohara, K.Kakiuchi, F.Kusu
    • Journal Title

      Anal.Biochem. 393

      Pages: 222-228

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An LC method for monitoring medium-chain fatty acid permeation through CaCo-2 cell monolayers2009

    • Author(s)
      K.Yamamoto, H.Hakamata, A.Yamaguchi, F.Kusu
    • Journal Title

      Anal.Bioanal.Chem. 395

      Pages: 533-538

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of short-chain fatty acids in rat and human feces by high-performance liquid chromatography with electrochemical detection2009

    • Author(s)
      A.Kotani, Y.Miyaguchi, M.Kohama, T.Ohtsuka, T.Shiratori, F.Kusu
    • Journal Title

      Anal.Sci. 25

      Pages: 1007-1011

    • NAID

      10025214039

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] コレステロールの電解酸化を利用する生体試料中のステロールの定量2011

    • Author(s)
      袴田秀樹, 小谷明, 楠文代
    • Organizer
      第57回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      沖縄県男女共同参画センター・沖縄県
    • Year and Date
      2011-12-01
    • Related Report
      2011 Final Research Report
  • [Presentation] イオン排除型セミミクロカラムを用いた電気化学検出HPLCによる便中有機酸の定量2011

    • Author(s)
      佐藤由季, 小谷明, 楠文代
    • Organizer
      第55回日本薬学会関東支部大会
    • Place of Presentation
      東邦大学・千葉県
    • Year and Date
      2011-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] セミミクロカラムを用いた電気化学検出HPLCによる血中遊離脂肪酸のモニタリング2011

    • Author(s)
      杉山秀人, 小谷明, 楠文代
    • Organizer
      第55回日本薬学会関東支部大会
    • Place of Presentation
      東邦大学・千葉県
    • Year and Date
      2011-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] バイオメディカル実分析を志向した電気化学分析法の開発2011

    • Author(s)
      楠文代
    • Organizer
      日本分析化学会第60回年会
    • Place of Presentation
      名古屋大学・愛知県
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] バイオメディカル実分析を志向した電気化学分析法の開発2011

    • Author(s)
      楠文代
    • Organizer
      日本分析化学会第60回年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 胆汁酸代謝関連物質の電気化学検出HPLC2011

    • Author(s)
      林優子, 袴田秀樹, 楠文代
    • Organizer
      Separation Sciences 2010
    • Place of Presentation
      幕張メッセ・千葉県
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] Electrochemical determination of weak acids based on the reduction of quinone2011

    • Author(s)
      F. Kusu, A. Kotani, K. Takamura, H. Hakamata
    • Organizer
      International Symposium on Electroanalytical Chemistry(Shikata Discussion 2011)
    • Place of Presentation
      淡路夢舞台国際会議場・兵庫県
    • Year and Date
      2011-05-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気化学検出HPLCによる大豆イソフラボン定量における分析時間の短縮2010

    • Author(s)
      松本大吾, 小谷明, 楠文代
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田大学・秋田県
    • Year and Date
      2010-11-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気化学検出HPLCによる大豆イソフラボン定量における分析時間の短縮2010

    • Author(s)
      松本大吾、小谷明、楠文代、
    • Organizer
      第56回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      秋田大学(秋田県)
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酸化ステロールの高感度分離定量法の開発と酸化LDL分析への応用2009

    • Author(s)
      垣内宏祐, 松永伊央, 袴田秀樹, 楠文代
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学・北海道
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 酸化ステロールの高感度分離定量法の開発と酸化LDL分析への応用2009

    • Author(s)
      垣内宏祐・松永伊央・袴田秀樹・楠文代
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Determination of plasma free fatty acids in diabetic rats by HPLC with electrochemical detection and the relation between FFAs and valproic Acid2009

    • Author(s)
      F. Kusu, T. Ohtsuka, N. Ishii, A. Kotani
    • Organizer
      12th the International Symposium on Electroanalytical Chemistry(12th ISEC)
    • Place of Presentation
      長春・中国
    • Year and Date
      2009-08-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Determination of isoflavones in soymilks by HPLC with electrochemical detection2009

    • Author(s)
      D. Matsumoto, A. Kotani, H. Hakamata, F. Kusu
    • Organizer
      12th the International Symposium on Electroanalytical Chemistry(12th ISEC)
    • Place of Presentation
      長春・中国
    • Year and Date
      2009-08-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Determination of plasma free fatty acids in diabetic rats by HPLC with electrochemical detection and the relation between FFAs and valproic Acid2009

    • Author(s)
      F.Kusu, T.Ohtsuka, N.Ishii, A.Kotani
    • Organizer
      12th the International Symposium on Electroanalytical Chemistry (12th ISEC)
    • Place of Presentation
      長春,中国
    • Year and Date
      2009-08-12
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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