Study on characteristic analysis and extension of application for auto-correlation spectroscopy

• Project/Area Number: 21550090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: FUJIWARA Kitao 東京薬科大学, 生命科学部, 教授 (90090521)
• Co-Investigator(Kenkyū-buntansha): AOKI Motohide 東京薬科大学, 生命科学部, 助教 (30418917)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 蛍光分光法 / 自己相関分光法 / 細胞 / 分子計測 / 蛍光相関法 / FCS / 粘度 / エタノール / 混合溶媒 / タンパク質 / 金属変性 / 大腸菌 / マイトマイシン / 細胞サイズ / クロロフィル / 円石藻 / 粘性抵抗 / 共焦点光学系 / 共焦点顕微分光 / 緑色蛍光タンパク質 / 溶媒の粘度 / 拡散時間 / シングルモード光ファイバー

Research Abstract

On the base of the previous study, the size variation of E. coli cell induced by MitomycinC(MMC) and Effect of Coccolith surrounding the cell of Pleurochrysis hatonemofera on its fluid dynamics were investigated with the ordinary fluorescent configuration. With con-focal fluorescent system, the viscosity of water/ethanol binary solution was inspected with dissolving Rhodamine 6G. In this study, auto-correlation spectroscopy was not investigated.

Research Products

• [Journal Article] Observation of cell-size variation under environmental stress by fluorescence correlation spectroscopy without objective image magnification (2012)
  • Author(s): Fujiwara K., Horiuchi, K., Goryouda, S., Hqashidume, Y., Horiguchi, Y., Miyakawa, T., Takasu, M., Aoki, M.
  • Journal Title: J. Environ. Sci. Eng. A(U. S. A.) Volume: 1 Pages: 364-370
• [Journal Article] Observation of cell-size variation under environmental stress by FCS (2012)
  • Author(s): Kitao Fujiwara(第1著者), 他7名
  • Journal Title: J.Environ.Sci.Engeneering.A Volume: 1(DOI不明のためISSNコードを記載) Pages: 364-370
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Kumata, H., Mori, M., Takahashi, S., Takamiya, S, Tsuzuki, M., Uchida, T., Fujiwara, K.
  • Journal Title: Environ. Sci. Technol Volume: 45 Pages: 9990-9997
• [Journal Article] Evaluation of hydrogenated resinacids as molecularmarkers (2011)
  • Author(s): Kitao Fujiwara, 7番目, 他6名
  • Journal Title: Environ.Sci.Technol. Volume: 23 Pages: 9990-9997
  • Peer Reviewed
• [Journal Article] Radiocarbon-based source apportionment of black carbon(BC) in PM10 aerosols from residential area of suburban Tokyo (2010)
  • Author(s): Uchida, M., Kumata, H., Koike, Y., Tsuzuki, M., Uchida, T., Fujiwara, K., Shibata, Y.
  • Journal Title: Nucl. Instrum. Methods B Volume: 268(7-8) Pages: 1120-1124
• [Journal Article] 天然水中有機物の光分解装置の制作と化学発光法を用いたその性能評価 (2010)
  • Author(s): 藤原祺多夫、大迫祐太、佐々木孝奈、熊田英峰、青木元秀、川島範男
  • Journal Title: 分析化学 Volume: 59 Pages: 1021-1028
• [Journal Article] 天然水中有機物の光分解装置の制作と化学発酵法を押いたその評価 (2010)
  • Author(s): 藤原祺多夫、大迫祐太、佐々木孝奈、熊田英峰、青木元秀、川島範
  • Journal Title: 分析化学 Volume: 59 Pages: 1021-1028
  • Peer Reviewed
• [Journal Article] 全フローシステムによる化学的酸素要求量の化学発光法による測定と簡便光分解装置性能評価の研究 (2010)
  • Author(s): 藤原祺多夫, 他
  • Journal Title: 分析化学 (印刷中)
  • Peer Reviewed
• [Presentation] Determination of size veriation of E coli under the environmental stress (2011)
  • Author(s): K.Fujiwara, 他4名(5番目)
  • Organizer: European Meeting for Environmental chemistry
  • Place of Presentation: Clermont-Ferrand
  • Year and Date: 2011-12-07
• [Presentation] Viscosity measurements of a water-ethanol binary solution with Rhodamine 6G by fluorescence correlation spectroscopy (2011)
  • Author(s): Horiuchi, K., Tsuyuki, A., Aoki, M., Fujiwara, K.
  • Organizer: 12th European Meeting on Environmental Chemistry
  • Place of Presentation: Clermont-Ferrand, France
• [Presentation] Determination of cell-size variation under the environmental stress by fluorescence correlation spectroscopy without image-magnification (2010)
  • Author(s): Horiuchi, K., Goryoda, S., Horiguchi, N., Aoki, M., Fujiwara, K.
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] The ultrasensitive real-time detection of the volatile organic compound with the nanocrystal-related semiconductor sensor (2010)
  • Author(s): Akiyama, J., Yanagisawa, S., Fujiwara, K., Uchida, T.
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] Thallium induces morphological changes in the photosynthetic membrane of Synechocystis sp. PCC6803 (2010)
  • Author(s): Matsumoto, H., Aoki, M., Takahashi, T., Sato, K., Kumata, H., Fujiwara, K.
  • Organizer: 15th International Congress of Photosynthesis
  • Place of Presentation: Beijing, China
• [Presentation] Gene Expression Analyses of Putative Chromate Transporters and Stress Response Factors under Chromate Exposure Condition in a Cyanobacteria (2010)
  • Author(s): Takenaka, K., Kikuchi, A., Aoki, M., Kumata, H., Fujiwara, K.
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] Determination of cell-size variation under the enzironmental stress by FCS (2010)
  • Author(s): K.Horiuchi, S.Goryoda, N.Horiguchi, M.Aoki, K.Fujiwara
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Protroz, Slovenia
• [Presentation] 蛍光相関法による大腸菌サイズのカルシウム依存性の測定 (2009)
  • Author(s): 藤原祺多夫, 他
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2009-09-26
• [Presentation] Development of the method for photo-decomposition and chemiluminescence evaluation of chemical oxygen demand (2009)
  • Author(s): Yuya Osako, Motohide Aoki, Hidetoshi Kumata, Kitao Fujiwara
  • Organizer: 10^<th> European Meeting on Environmental Chemistry(EMEC10)
  • Place of Presentation: Limoges, France
• [Presentation] Toxicity of cobalt complex isomers to micro-algae (2009)
  • Author(s): Sa-ai, N., Midorikawa, M., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Analysis of proteins responded to thallium exposure in Synechocystis sp. PCC6803 (2009)
  • Author(s): Takahashi, T., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Identification of stress response genes to hexavalent chromium in cyanobacterium Synechocystis sp. PCC6803 (2009)
  • Author(s): Takenaka, K., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Analysis of the transporters of hexavalent chromium in cyanobacterium Synechocystis sp. PCC6803 (2009)
  • Author(s): Kikuchi, A., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Thallium Induces Morphological Changes in the Photosynthetic Apparatus of Synechocystis sp. PCC6803
  • Author(s): Aoki, M., Matsumoto, H., Takahashi, T., Sato, K., Kumata, H., Fujiwara, K.
  • Organizer: In the PS2010 Proceedings, 15th International Congress on Photosynthesis Held
  • Place of Presentation: Beijing, China
• [Book] わかる×わかった環境化学 (2011)
  • Author(s): 斎藤勝裕、藤原祺多夫
  • Publisher: オーム社
• [Book] わかるXわかった!環境化学 (2011)
  • Author(s): 斎藤勝裕、藤原祺多夫
  • Total Pages: 236
  • Publisher: オーム社