Development of sensitive analytical method for protein and nucleic acid using natural elemental tags

• Project/Area Number: 21550094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAKATSU Akiko 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究科長 (10357361)
• Co-Investigator(Kenkyū-buntansha): INAGAKI Kazumi 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究室付 (50356490) ; FUJII Shin-ichiro 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究員 (10415739)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生体分析 / 核酸 / タンパク質 / 誘導結合プラズマ質量分析 / 液体クロマトグラフィー / キャピラリー電気泳動 / リン定量

Research Abstract

A high performance interface device was developed for sample introduction to inductively coupled plasma mass spectrometer(ICP-MS) at low flow rate. Using this interface device, hyphenation of capillary liquid chromatography(LC) or capillary electrophoresis with ICP-MS was achieved. DNA molecules separated with CE or capillary LC were sensitively and selectively detected with ICP-MS in quantification of phosphorus as an elemental tag. We have concluded that this system can provide a fast screening method for various bio-molecules in biological samples.

Research Products

• [Journal Article] High Performance Concentric Nebulizer for Low-Flow Rate Liquid Sample Introduction to ICP-MS (2011)
  • Author(s): 稲垣和三、藤井紳一郎、高津章子、千葉光一
  • Journal Title: Journal of Analytical Atomic Spectrometry Volume: Vol.26(3) Issue: 3 Pages: 623-630
  • DOI: 10.1039/c0ja00128g
  • Peer Reviewed
• [Journal Article] Quantification of phosphorus in DNA using capillary electro-phoresis hyphenated with inductively coupled plasma mass spectrometr (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Journal Title: JOURNAL OF CHROMATOGRAPHY A Volume: Vol.1217(50) Issue: 50 Pages: 7921-7925
  • DOI: 10.1016/j.chroma.2010.10.066
  • Peer Reviewed
• [Journal Article] Quantification of phosphorus in DNA using capillary electro-phoresis hyphenated with inductively coupled plasma mass spectrometry (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Journal Title: JOURNAL OF CHROMATOGRAPHY A Volume: 1217(50) Pages: 7921-7925
  • Peer Reviewed
• [Presentation] 核酸分子の微少定量法および質量分析法を用いたATP放射線障害の分析 (2012)
  • Author(s): 藤井紳一郎
  • Organizer: 第461回基礎科学セミナー
  • Place of Presentation: 茨城県、日本原子力研究開発機構
  • Year and Date: 2012-01-10
• [Presentation] 核酸分子の微少定量法および質量分析法を用いたATP放射線障害の分析 (2012)
  • Author(s): 藤井紳一郎
  • Organizer: 第461回基礎科学セミナー
  • Place of Presentation: 日本原子力研究開発機構(茨城県)
  • Year and Date: 2012-01-10
• [Presentation] DNA Analysis by Using Capillary Gel Electrophoresis Hyphenated with ICP-MS (2011)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Organizer: 2011 European Winter Conference on Plasma Spectrochemistry
  • Place of Presentation: Zaragoza, Spain
  • Year and Date: 2011-02-03
• [Presentation] 微少試料導入系を用いたCE-誘導結合プラズマ質量分析装置による核酸の分析 (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Organizer: 第30回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 岐阜県岐阜市
  • Year and Date: 2010-11-17
• [Presentation] 分子内リンを指標としたキャピラリー電気泳動-ICP-MSによるDNA定量技術の開発 (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2010-09-15
• [Presentation] 分子内リンを指標としたキャピラリー電気泳動-ICP-MSによるDNA定量技術の開発 (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-15
• [Presentation] Determination of Phosphorus Content in DNA Using Capillary Electrophoresis Hyphenated with Inductively Coupled Plasma Mass Spectrometry (2010)
  • Author(s): 藤井紳一郎、稲垣和三、千葉光一、高津章子
  • Organizer: Microscale Bioseparations(MSB) 2010
  • Place of Presentation: Prague, Czech
  • Year and Date: 2010-03-22
• [Presentation] Determination of Phosphorus Content in DNA Using Capillary Electrophoresis Hyphenated with Inductively Coupled Plasma Mass Spectrometry (2010)
  • Author(s): 藤井紳一郎, 稲垣和三, 千葉光一, 高津章子
  • Organizer: MSB2010
  • Place of Presentation: Prague, Czech
  • Year and Date: 2010-03-22
• [Presentation] 高機能試料導入系が切り開くICP-MSの新展開 (2009)
  • Author(s): 稲垣和三
  • Organizer: 第6回茨城地区分析技術交流会
  • Place of Presentation: 那珂、茨城
  • Year and Date: 2009-12-04
• [Presentation] 微少試料導入系が切り開くLC-ICP-MSの新展開 (2009)
  • Author(s): 稲垣和三
  • Organizer: 第222回液体クロマトグラフィー研究懇談会
  • Place of Presentation: 神田、東京
  • Year and Date: 2009-06-23