Development of a new method for peptide ligation based on the N-S acyl shift reaction
| Project/Area Number |
21550155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
KAWAKAMI Toru 大阪大学, 蛋白質研究所, 准教授 (70273711)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 蛋白質 / ペプチド / 合成化学 / ライゲーション / ペプチドチオエステル / 生体分子 / 有機化学 / ケミカルライゲーション / N-Sアシル基転移反応 / ジケトピペラジン / N-Sアシル基転位反応 |
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| Research Abstract |
The peptide thioester is used as a building block for protein synthesis. In this research, we developed new methods for the peptide thioester synthesis. Based on the structure of Cys-Pro ester(CPE), Cys-Pro-Cys sequence was found to produce a peptide thioester. It could be prepared as a recombinant protein. When N-substitute Gly residue is introduced instead of the Pro residue in the CPE structure, this CNGE peptide produces the thioester more efficiently than the CPE peptide.
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Report
(4 results)
Research Products
(49 results)
