| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
The peptide thioester is used as a building block for protein synthesis. In this research, we developed new methods for the peptide thioester synthesis. Based on the structure of Cys-Pro ester(CPE), Cys-Pro-Cys sequence was found to produce a peptide thioester. It could be prepared as a recombinant protein. When N-substitute Gly residue is introduced instead of the Pro residue in the CPE structure, this CNGE peptide produces the thioester more efficiently than the CPE peptide.
|
