Characterization of heme transport proteins from pathogenic bacteria

• Project/Area Number: 21550157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Yamaguchi University
• Principal Investigator: OZAKI Shinichi 山口大学, 農学部, 教授 (40280581)
• Co-Investigator(Kenkyū-buntansha): MIGITA Taiko 山口大学, 農学部, 教授 (90159161)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ヘム / 受容体 / トランスポーター / ESR / 共鳴ラマン / 配位構造

Research Abstract

Iron is involved in a wide range of essential functions in various metabolic pathways ; therefore, most bacteria have some form of iron-scavenging system in order to survive. Heme acquisition system A(HasA), secreted by various gram-negative pathogens, uses a unique histidine-iron-tyrosine(His-Fe-Tyr) or an iron-tyrosine(Fe-Tyr) coordination to scavenge heme. Our results suggest that the binding of heme in apoprotein is initiated by interactions between heme and hydrophobic residues in the heme binding pocket. Subsequent coordination of Tyr to the heme iron complete holoprotein formation.

Research Products

• [Journal Article] Regioselective glucosylation of trans-resveratrol in recombinant Escherichia coli expressing glucosyltransferase from Phytolacca Americana (2012)
  • Author(s): Ozaki, S., Imai, H., Iwakiri, T., Sato, T., Shimoda, K., Nakayama, T., Hamada, H.
  • Journal Title: Biotechnology Letters Volume: 34 Pages: 475-481
  • Peer Reviewed
• [Journal Article] Mechanism of heme uptake by heme acquisition system A (2011)
  • Author(s): Ozaki, S., Nakahara, A., Sato, T.
  • Journal Title: Chemistry Letters Volume: 40 Pages: 362-363
  • NAID: 10028170421
  • Peer Reviewed
• [Journal Article] Mutagenesis studies of human cystathionineβ-Synthase : Residues important for heme binding and substrate interactions (2010)
  • Author(s): Ozaki, S., Sakaguchi, C., Nakahara, A., and Yoshiya, M.
  • Journal Title: Protein & Peptide Letters Volume: 17 Pages: 351-355
  • Peer Reviewed
• [Journal Article] A heterodimeric cytochrome c complex with a very low redox potential from an anaerobic ammonium-oxidizing enrich (2010)
  • Author(s): Ukita, S., Fujii, T., Hira, D., Nishiyama, T., Kawase, T., Migita, C. T., Furukawa, K.
  • Journal Title: FEMS Microbiology Letters Volume: 313 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Mutagenesis studies of human cystathionine β-synthase : Residues important for heme binding and substrate interactions (2010)
  • Author(s): Ozaki, S, Sakaguchi C, Nakahara A, Yoshiya M
  • Journal Title: Protein & Peptide Letters Volume: 17 Pages: 351-355
  • Peer Reviewed
• [Journal Article] Mutagenesis studies of human cystathionine β-synthase : residues important for heme binding and substrate interactions (2010)
  • Author(s): S.Ozaki
  • Journal Title: Protein & Peptide Letters 17 Pages: 351-355
  • Peer Reviewed
• [Journal Article] Biochemical and spectroscopic properties of cyanide-insensitive quinol oxidase from Gluconobacter oxydans (2009)
  • Author(s): Mogi, T., Ano, Y., Nakatsuka, T., Toyama, H., Muroi, A., Miyoshi, H., Migita, C. T., Ui, H., Shiomi, K., Omura, S.
  • Journal Title: Journal of Biochemistry Volume: 146 Pages: 263-271
  • Peer Reviewed
• [Journal Article] Mutagenesis of Gln-142 and Phe-143 of O-acetylserine sulfhydrylase (2009)
  • Author(s): S.Ozaki
  • Journal Title: Asian Journal of Biochemistry 4 Pages: 117-124
  • Peer Reviewed
• [Presentation] 病原性微生物によるヘムの輸送機構 (2011)
  • Author(s): 小崎紳一
  • Organizer: 錯体討論会
  • Place of Presentation: 岡山理科大学岡山市
  • Year and Date: 2011-09-17
• [Presentation] 病原性微生物によるヘムの輸送機構 (2011)
  • Author(s): 小崎紳一
  • Organizer: 錯体討論会
  • Place of Presentation: 岡山理科大学岡山
  • Year and Date: 2011-09-17
• [Presentation] 病原性微生物によるヘム捕捉機構 (2011)
  • Author(s): 小崎紳一
  • Organizer: 生体分子科学討論会
  • Place of Presentation: 筑波大学筑波市
  • Year and Date: 2011-06-23
• [Presentation] 病原性微生物によるヘム捕捉機構 (2011)
  • Author(s): 小崎紳一
  • Organizer: 生体分子科学討論会
  • Place of Presentation: 筑波大学筑波
  • Year and Date: 2011-06-23
• [Presentation] ヘムを捕捉するタンパクの機能とスペクトル (2010)
  • Author(s): 佐藤豪洋, 小崎紳一
  • Organizer: 錯体化学討論会
  • Place of Presentation: 大阪国際交流センター大阪市
  • Year and Date: 2010-09-27
• [Presentation] ヘムを捕捉するタンパクの機能とスペクトル (2010)
  • Author(s): 小崎紳一
  • Organizer: バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学大阪市
  • Year and Date: 2010-09-26
• [Presentation] ヘムを捕捉するタンパクの機能とスペクトル (2010)
  • Author(s): 小崎紳一、佐藤豪洋、中原章、岩切智也、重政彰徳、松田尚子
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-24
• [Presentation] Heme acquisition system A(HasA)の機能とスペクトル (2010)
  • Author(s): 小崎紳一
  • Organizer: 生体分子科学討論会
  • Place of Presentation: 山口大学山口市
  • Year and Date: 2010-06-19
• [Presentation] Heme acquisition system A(HasA)によるヘム獲得機構について (2010)
  • Author(s): 小崎紳一
  • Organizer: 日本生化学会中国・四国支部例会
  • Place of Presentation: 山口大学山口市
  • Year and Date: 2010-05-15
• [Presentation] 緑膿菌由来のヘム結合タンパクに関する研究 (2009)
  • Author(s): 中原章, 小崎紳一
  • Organizer: 生体関連化学シンポジウム
  • Place of Presentation: 九州大学福岡市
  • Year and Date: 2009-09-14
• [Presentation] Redox-dependent change in the coordination of heme acquisition system A (2009)
  • Author(s): Ozaki, S.
  • Organizer: International Conference on Bioinorganic Chemistry
  • Place of Presentation: Nagoya
  • Year and Date: 2009-07-29
• [Presentation] Redox-dependent changes in the coordination of heme acquisition system (2009)
  • Author(s): S.Ozaki
  • Organizer: International Conference on Biological Inorganic Chemistry 14
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan,