Development of new cross-linkers to detect DNA-protein interaction
| Project/Area Number |
21550164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANAI Kazuyuki 東邦大学, 理学部, 准教授 (30360704)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生体機能関連化学 / 転写因子機能解析 / 転写因子 / DNA-タンパク質相互作用 / クロスリンカー / クロマチン免疫沈降法 / 光反応 |
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| Research Abstract |
Design and synthesis of new cross-linkers having both DNA and protein binding moieties have been developed. Expression vectors for fusion protein with SNAP tag were also established. Binding experiment of new cross-linkers with fusion proteins as well as a DNA duplex was carried out. In both cases, a new band was observed after gel electrophoresis, suggesting that cross-linkers can make a covalent bond with biomolecules of our interest.
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Report
(4 results)
Research Products
(51 results)
