| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Design and synthesis of new cross-linkers having both DNA and protein binding moieties have been developed. Expression vectors for fusion protein with SNAP tag were also established. Binding experiment of new cross-linkers with fusion proteins as well as a DNA duplex was carried out. In both cases, a new band was observed after gel electrophoresis, suggesting that cross-linkers can make a covalent bond with biomolecules of our interest.
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