Action of antimicrobial peptides into the bacterial outer membrane, activity enhancement, and the technology for single molecular evaluation
Project/Area Number |
21550168
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
FUKUOKA Satoshi 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (40357885)
|
Co-Investigator(Kenkyū-buntansha) |
ITO Tamitake 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (00351742)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 抗菌ペプチド / リポ多糖 / リピドA / 胆汁酸 / 表面増強ラマン分光 / 単一分子解析 / 高次構造 / 生物活性 / 表面増強ラマン散乱 / リムルス試薬 |
Research Abstract |
The interaction of lipopolysaccharide(LPS) and its hydrophobic part lipid A with antimicrobial peptides or bile acids was studied. We elucidated the conformation of their phosphate groups due to peptide binding, and the profile of the secondary structure of the peptides by physicochemical analyses. The thermodynamics of binding between peptides and lipid A showed that the acidic amino acid substituted peptides improved the binding activity. The conformation change of the peptide enhanced the ability of incorporation into the lipid A aggregates, along with changes in biochemical and biophysical parameters. The surface enhanced Raman scattering spectroscopy study indicated the applicability of that to the LPS evaluation.
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Report
(4 results)
Research Products
(20 results)
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[Presentation] リポ多糖の膜構造や生物活性を変化させる生体関連分子2012
Author(s)
福岡聰, Walter Richter, Jorg Howe, Jorg Andra, Manfred Rossle, Christian Alexander, Thomas Gutsmann, and Klaus Brandenburg
Organizer
日本化学会第92春季年会
Place of Presentation
神奈川県・慶応義塾大学
Year and Date
2012-03-25
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[Presentation] 胆汁酸によるリポ多糖の膜構造変化と生物活性2011
Author(s)
福岡聰, Walter Richter, Jorg Howe, Jorg Andra, Manfred Rossle, Christian Alexander, Thomas Gutsmann, Klaus Brandenburg
Organizer
日本化学会第91春季年会(要旨集及びWebにて)
Year and Date
2011-03-26
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[Presentation] エンドトキシンの両親媒性ペプチドによる活性中和の分子機構2010
Author(s)
Klaus Brandenburg, Patrick Garidel, Satoshi Fukuoka, Jorg Howe, Michel H. J. Koch, Thomas Gutsmann, and Jorg Andra
Organizer
平成21年度産技連LsBt合同発表会
Place of Presentation
茨城県・産業技術総合研究所
Year and Date
2010-02-04
Related Report
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[Presentation] エンドトキシンの両親媒性ペプチドによる活性中和の分子機構2010
Author(s)
Klaus Brandenburg, Patrick Garidel, Satoshi Fukuoka, Jorg Howe, Michel H.J.Koch, Thomas Gutsmann, Jorg Andra
Organizer
平成21年度産技連LsBt合同発表会
Place of Presentation
産総研つくばセンター
Year and Date
2010-02-04
Related Report