| Project/Area Number |
21550199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Hideki 聖マリアンナ医科大学, 医学部, 教授 (20192669)
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| Co-Investigator(Renkei-kenkyūsha) |
URYU Toshiyuki (80011005)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | カードラン硫酸 / アルキル鎖導入カードラン硫酸 / インフルエンザウイルス / メンブランフィルター / 静電的相互作用 / HA価 / インフルエンザ / アルキル鎖 / 疎水的相互作用 / 抗ウイルス性 / 吸着 |
|---|
| Research Abstract |
To develop a biomaterial with an influenza virus-adsorptive functionality, an alkyl curdlan sulfate was prepared by ionic interaction between a positively charged didodecyldimethyl ammonium bromide and a negatively charged sulfate group of curdlan sulfate, which has potent anti-HIV activity, and then coated on a membrane filter with a 1-μm pore size by hydrophobic interaction with the long alkyl groups in the curdlan sulfate. The alkyl curdlan sulfate with the degree of alkylation (DOA) of 0.03 (1 didodecyldimethyl group/12 sugar residues with 36 hydroxyl or sulfate groups) showed potent anti-HIV activity in a 50% effective concentration (EC_<50>) as low as 0.87μg/ml (standard curdlan sulfate EC_<50>=0.3μg/ml). When 1.6 mg of the alkyl curdlan sulfate with the DOA of 0.03 was coated on a membrane filter (13 mmΦ, pore size : 1μm), three stacked alkyl curdlan sulfate-coated membrane filters dramatically decreased hemagglutination to 1/4-1/32. These results can therefore be presumed to demonstrate that the alkyl curdlan sulfate-coated membrane filter removed influenza A viruses by adsorption between the negatively charged sulfate groups and the positively charged envelope protein of the virus.
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