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Development of a bioreactor with high growth efficiency for an indifferent cellar as a stem cell by using the chaos mixing Taylor vortex flow

Research Project

Project/Area Number 21560163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fluid engineering
Research InstitutionMuroran Institute of Technology

Principal Investigator

KAWAI Hideki  室蘭工業大学, 大学院・工学研究科, 教授 (20292071)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Hirotoshi  筑波大学, 人間総合科学研究科, 講師 (70292547)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsTaylor渦 / 固気液分離 / カオス / 混相流 / 微生物流動 / レーザードップラ流速計 / 超音波ドップラ流速計
Research Abstract

Ultrasound velocity measuring system for a Taylor Vortex flow(TVF) was developed with a small aspect ratio, where the chaotic complicated flow was generated because of the Ekman boundary layer near the upper and lower boundary conditions. Bioreactors are one of the practical applications for an TVF which enables a milder mixing process protecting the animal cell from the damage of higher share flows. Successive instantaneous and mean velocity profiles were obtained by using an Ultrasound doppler Velocity Profiler (UVP). An Ultrasound Time Domain Correlation method (UTDC) is another practical method for measurement of the concentration distribution in the solid liquid multiphase flows as well as the velocity measurement, however the present measuring ability is not high compared with the UVP method. In the study, we focused on the development for grading up of the UTDC measuring system and partly the spatiotemporal velocity field was carried out with the spectrum analysis of the Fourier transform, which resulted in good agreement with the one from the UVP.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (49 results)

All 2012 2011 2010 2009

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (34 results)

  • [Journal Article] 低アスペクト比Taylor渦流ないにおける光合成微生物の増殖特性2011

    • Author(s)
      野村慶太, 河合秀樹, 岡元隆典
    • Journal Title

      寒地技術シンポジウム論文集

      Volume: vol.27 Pages: 137-140

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional culture of mouse bone marrow cells on stroma formed within a porous scaffold : influence of scaffold shape and cryopreservation of the stromal layer on expansion of haematopoietic progenitor cells2011

    • Author(s)
      H.Miyoshi, N.Ohshima and C. Sato
    • Journal Title

      J. Tissue Eng Regen Med

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 超音波計測法によるアスペスト比の小さいTaylor-Couette渦流れの速度計測2011

    • Author(s)
      河合秀樹, 木倉宏成, 有冨正憲
    • Journal Title

      化学工学論文集

      Volume: vol.37 Pages: 85-90

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional culture of mouse bone marrow cells on stroma formed within a porous scaffold : influence of scaffold shape and cryopreservat ion of the stromal layer on expansion of haematopoietic progenitor cells2011

    • Author(s)
      H.Miyoshi, N.Ohshima, C.Sato
    • Journal Title

      J.Tissue Eng Regen Med

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超音波計測法によるアスペクト比の小さいTaylor-Couette渦流れの速度計測2011

    • Author(s)
      河合秀樹, 木倉宏成, 有冨正憲
    • Journal Title

      化学工学論文集

      Volume: 37 Pages: 85-90

    • NAID

      10027970588

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional perfusion cultures of mouse and pig fetal liver cells in a packed-bed reactor : Effect of medium flow rate on cell numbers and hepatic functions2010

    • Author(s)
      Hirotoshi Miyoshi, Tomo Ehashi, Hideki Kawai, Norio Ohshima, Satoshi Suzuki
    • Journal Title

      Journal of Biotechnology

      Volume: vol.148 Pages: 226-232

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 亜臨界水による植物性油脂の加水分解2010

    • Author(s)
      河合秀樹
    • Journal Title

      寒地技術論文集

      Volume: vol.26 Pages: 176-179

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成微生物の限界光量並びにCO2通気方法による増殖特性の違いについて2010

    • Author(s)
      野村慶太, 河合秀樹
    • Journal Title

      寒地技術論文集

      Volume: vol.26 Pages: 180-183

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional perfusion cultures of mouse and pig fetal liver cells in a packed-bed reactor: Effect of medium flow rate on cell numbers and hepatic functions2010

    • Author(s)
      Hirotoshi Miyoshi, Tomo Ehashi, Hideki Kawai, Norio Ohshima, Satoshi Suzuki
    • Journal Title

      Journal of Biotechnology

      Volume: 148 Pages: 226-232

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 寒冷地向きプロペラ型風力タービンに関する研究2009

    • Author(s)
      岸浪紘機・戸倉郁夫・河合秀樹・ヒムサール・アムバリタ・長船康裕・鈴木淳・阿部壮史・ノル・アズラン
    • Journal Title

      寒地技術論文集

      Volume: vol.25 Pages: 463-468

    • NAID

      120004681131

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] カウンターフロー型熱交換器の性能向上に関する研究2009

    • Author(s)
      岸浪紘機, ヒムサール・アムバリタ, 河合秀樹, 媚山政良, 戸倉郁夫, 長船康裕, 鈴木淳, 佐藤和彦, 掛川徹, 船越雅人
    • Journal Title

      寒地技術論文集

      Volume: vol.25 Pages: 169-173

    • NAID

      120004681132

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Optimum Plate to Plate Spacing for Maximum Heat Transfer Rate from a Flat Plate Type Heat Exchanger2009

    • Author(s)
      H. AMBARITA, K. KISHINAMI, M. DAIMARUYA, I. TOKURA, H. KAWAI, J. SUZUKI, M. KOBIYAMA, and A. GINTING
    • Journal Title

      Thermal Science & Engineering

      Volume: vol.17 Pages: 1-8

    • NAID

      10025228010

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Optimum Plate to Plate Spacing for Maximum Heat Transfer Rate from a Flat Plate Type Heat Exchanger2009

    • Author(s)
      H.AMBARITA, K.KISHINAMI, M.DAIMARUYA, I.TOKURA, H.KAWAI, J.SUZUKI, M.KOBIYAMA, A.GINTING
    • Journal Title

      Thermal Science & Engineering 17

      Pages: 1-8

    • NAID

      10025228010

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] カウンターフロー型熱交換器の性能向上に関する研究2009

    • Author(s)
      岸浪紘機, ヒムサール・アムバリタ, 河合秀樹, 媚山政良, 戸倉郁夫, 長船康裕, 鈴木淳, 佐藤和彦, 掛川徹, 船越雅人
    • Journal Title

      寒地技術論文・報告集 25

      Pages: 169-173

    • NAID

      120004681132

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 寒冷地向きプロペラ型風力タービンに関する研究2009

    • Author(s)
      岸浪紘機・戸倉郁夫・河合秀樹・ヒムサール・アムバリタ・長船康裕・鈴木淳・阿部壮史・ノル・アズラン
    • Journal Title

      寒地技術論文・報告集 25

      Pages: 463-468

    • NAID

      120004681131

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 超音波によるアスペクト比の小さいTaylor-Couette渦の解析2012

    • Author(s)
      本間友基, 河合秀樹
    • Organizer
      第21回化学工学・粉体工学研究会
    • Place of Presentation
      登別
    • Year and Date
      2012-01-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Taylor Couette渦を利用した固液分離濾過性能の評価実験2012

    • Author(s)
      河津秀治, 河合秀樹
    • Organizer
      第21回化学工学・粉体工学研究会
    • Place of Presentation
      登別
    • Year and Date
      2012-01-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Immobilization of the photosynthesis microorganism using polyethyleneimine-coated BSP in a Taylor-Couette vortex flow2012

    • Author(s)
      M.Nakamura, H.Kawai
    • Organizer
      MIER2012
    • Place of Presentation
      Noboribetsu
    • Year and Date
      2012-01-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Immobilization of the photosynthesis microorganism in the Taylor Couette vortex flow2012

    • Author(s)
      K. Nomura, H. Kawai
    • Organizer
      MIER2012
    • Place of Presentation
      Noboribetsu
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Immobilization of the photosynthesis microorganism using poly- ethyleneimine-coated BSP in a Taylor -Couette vortex flow2012

    • Author(s)
      M.Nakamura, H.Kawai
    • Organizer
      MIER2012
    • Place of Presentation
      Noboribetsu
    • Related Report
      2011 Final Research Report
  • [Presentation] Taylor Couette渦を利用した固液分離濾過性能の評価実験2012

    • Author(s)
      河津秀治,河合秀樹
    • Organizer
      第21回化学工学・粉体工学研究会
    • Place of Presentation
      登別市
    • Related Report
      2011 Final Research Report
  • [Presentation] 超音波によるアルペスト比の小さいTaylor-Couette渦の解析2012

    • Author(s)
      本間友基,河合秀樹
    • Organizer
      第21回化学工学・粉体工学研究会
    • Place of Presentation
      登別市
    • Related Report
      2011 Final Research Report
  • [Presentation] 低アスペクト比Taylor渦流内における光合成微生物の増殖特性2011

    • Author(s)
      野村慶太, 河合秀樹
    • Organizer
      第27回寒地技術シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2011-11-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Taylor-Couette渦バイオリアクターにおける光合成微生物の固定化の効果について2011

    • Author(s)
      中村恵龍, 河合秀樹
    • Organizer
      第43回化学工学会秋季大会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低アスペクト比Taylor渦流内における光合成微生物の増殖特性2011

    • Author(s)
      岡元隆典, 河合秀樹, 高橋洋志
    • Organizer
      第20回化学工学・粉体工学研究会
    • Place of Presentation
      札幌
    • Year and Date
      2011-02-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低アルペスト比Taylor渦流内における光合成微生物の増殖特性2011

    • Author(s)
      野村慶太,河合秀樹
    • Organizer
      第27回寒地技術シンポジウム
    • Place of Presentation
      札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] Taylor-Couette渦バイオリアクターにおける光合成微生物の固定化の効果について2011

    • Author(s)
      中村恵龍,河合秀樹
    • Organizer
      第43回化学工学会秋季大会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] 低アルペスト比Taylor渦流内における光合成微生物の増殖特性2011

    • Author(s)
      岡元隆典,河合秀樹,高橋洋志
    • Organizer
      第20回化学工学・粉体工学研究会
    • Place of Presentation
      札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] アスペクト比の小さいTaylor-Couette渦の数値解析2010

    • Author(s)
      河合秀樹, 斎藤義鷹
    • Organizer
      日本機械学会北海道支部第49回講演会
    • Place of Presentation
      札幌
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] アスペクト比の小さいTaylor-Couette渦による光合成微生物の増殖特性2010

    • Author(s)
      河合秀樹, 岡元隆典, 野村慶太
    • Organizer
      日本機械学会北海道支部第49回講演会
    • Place of Presentation
      札幌
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] アスペクト比の小さいTaylor渦流れの解析2010

    • Author(s)
      河合秀樹, 長井明寛, 木倉宏成
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      米沢
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超音波によるTaylor渦固液二相流の速度と濃度計測の可能性について2010

    • Author(s)
      河合秀樹, 木倉宏成
    • Organizer
      日本流体力学会年会2010
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Taylor渦における光合成微生物の増殖特性について2010

    • Author(s)
      河合秀樹, 岡元隆典
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      京都
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超音波によるTaylor-Couette渦の固液混合流れの速度計測2010

    • Author(s)
      河合秀樹, 斎藤義鷹, 木倉宏成
    • Organizer
      第38回可視化情報シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-07-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] アルペスト比の小さいTaylor-Couette渦の数値解析2010

    • Author(s)
      河合秀樹,斎藤義鷹
    • Organizer
      日本機械学会北海道支部第49回講演会
    • Place of Presentation
      札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] アルペスト比の小さいTaylor-Couette渦による光合成微生物の増殖特性2010

    • Author(s)
      河合秀樹,岡元隆典,野村慶太
    • Organizer
      日本機械学会北海道支部第49回講演会
    • Place of Presentation
      札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] アルペスト比の小さいTaylor渦流れの解析2010

    • Author(s)
      河合秀樹,長井明寛,木倉宏成
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      米沢市
    • Related Report
      2011 Final Research Report
  • [Presentation] 超音波によるTaylor渦固液二相流の速度と濃度計測の可能性について2010

    • Author(s)
      河合秀樹,木倉宏成
    • Organizer
      日本流体力学会年会2010
    • Place of Presentation
      札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] Taylor渦における光合成微生物の増殖特性について2010

    • Author(s)
      河合秀樹,岡元隆典
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      京都市
    • Related Report
      2011 Final Research Report
  • [Presentation] 超音波によるTaylor-Couette渦の固液混合流れの速度計測2010

    • Author(s)
      河合秀樹,斎藤義鷹,木倉宏成
    • Organizer
      第38回可視化情報シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 磁性流体によるアスペクト比の小さいTaylor渦流れの数値解析2009

    • Author(s)
      河合秀樹, 長井明寛, 井門康司, 木倉宏成
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 超音波計測によるアスペクト比の小さいTaylor渦流れの解析2009

    • Author(s)
      河合秀樹, 小林広精, 木倉宏成, 有冨正憲
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] アスペクト比の小さいTaylor渦による光合成微生物の増殖特性について2009

    • Author(s)
      河合秀樹, 安井砂雄, 岡元隆典
    • Organizer
      化学工学会第41回秋季大会
    • Place of Presentation
      広島市
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Taylor-Couette渦流れにおける光合成微生物の培養実験2009

    • Author(s)
      河合秀樹,安井砂雄,高橋秀治,木倉宏成,有冨正憲
    • Organizer
      第37回可視化情報シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 超音波によるTaylor-Couette渦の固液混合濃度分布の計測2009

    • Author(s)
      河合秀樹,新部智由,木倉宏成,有冨正憲
    • Organizer
      第37回可視化情報シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] アルペスト比の小さいTaylor渦による光合成微生物の増殖特性について2009

    • Author(s)
      河合秀樹,安井砂雄,岡元隆典
    • Organizer
      化学工学会第41回秋季大会
    • Place of Presentation
      広島市
    • Related Report
      2011 Final Research Report
  • [Presentation] 超音波自己相関法によるTaylor渦の速度分布計測2009

    • Author(s)
      河合秀樹,小林広精,新部智由
    • Organizer
      化学工学会第41回秋季大会
    • Place of Presentation
      広島市
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 超音波計測によるアルペスト比の小さいTaylor渦流れの解析2009

    • Author(s)
      河合秀樹,小林広精,木倉宏成,有冨正憲
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] 磁性流体によるアルペスト比の小さいTaylor渦流れの数値解析2009

    • Author(s)
      河合秀樹,長井明寛,井門康司,木倉宏成
    • Organizer
      日本機械学会流体工学部門講演会
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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