Monitoring of cell membrane disruption and recovery by broadband dielectric spectroscopy
| Project/Area Number |
21560447
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Measurement engineering
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ASAMI Koji 京都大学, 化学研究所, 准教授 (90127936)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 計測システム / 誘電分光 / 細胞膜 / 赤血球 / 界面分極 / 溶血 / 細胞 / 誘電緩和 |
|---|
| Research Abstract |
A suspension of erythrocyte ghosts obtained by hypotonic hemolysis shows dielectric dispersion termedα-dispersion below 10 kHz. The exact nature of theα-dispersion, however, has not been studied because of difficulty in low-frequency measurement due to electrode polarization, and therefore its origin has not been clearly understood. In this study, a new electrode configuration was designed to extend the available frequency region to a lower frequency side, making it possible to investigate theα-dispersion in detail. The occurrence of theα-dispersion correlated strongly with the presence of holes in the ghost membranes and its properties were exactly interpreted by the numerical simulation with the spherical membrane model with a hole. The numerical simulation provided a liner relation between the characteristic frequency of theα-dispersion and the hole radius, and thereby the hole radius being determined straightforwardly.
|
Report
(4 results)
Research Products
(17 results)
