• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Monitoring of cell membrane disruption and recovery by broadband dielectric spectroscopy

Research Project

Project/Area Number 21560447
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Measurement engineering
Research InstitutionKyoto University

Principal Investigator

ASAMI Koji  京都大学, 化学研究所, 准教授 (90127936)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords計測システム / 誘電分光 / 細胞膜 / 赤血球 / 界面分極 / 溶血 / 細胞 / 誘電緩和
Research Abstract

A suspension of erythrocyte ghosts obtained by hypotonic hemolysis shows dielectric dispersion termedα-dispersion below 10 kHz. The exact nature of theα-dispersion, however, has not been studied because of difficulty in low-frequency measurement due to electrode polarization, and therefore its origin has not been clearly understood. In this study, a new electrode configuration was designed to extend the available frequency region to a lower frequency side, making it possible to investigate theα-dispersion in detail. The occurrence of theα-dispersion correlated strongly with the presence of holes in the ghost membranes and its properties were exactly interpreted by the numerical simulation with the spherical membrane model with a hole. The numerical simulation provided a liner relation between the characteristic frequency of theα-dispersion and the hole radius, and thereby the hole radius being determined straightforwardly.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010 2009

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (7 results)

  • [Journal Article] Dielectric spectroscopy reveals nanoholes in erythrocyte ghosts2012

    • Author(s)
      K. Asami
    • Journal Title

      Soft Matter

      Volume: 8 Issue: 11 Pages: 3250-3257

    • DOI

      10.1039/c2sm06306a

    • NAID

      120005241350

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design of a measurement cell for low-frequency dielectric spectroscopy of biological cell suspensions2011

    • Author(s)
      K. Asami
    • Journal Title

      Measurement Science and Technology

      Volume: 22 Issue: 8 Pages: 085801-085801

    • DOI

      10.1088/0957-0233/22/8/085801

    • NAID

      120003338847

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dielectric properties of microvillous cells simulated by the three-dimensional finite-element method2011

    • Author(s)
      K. Asami
    • Journal Title

      Bioelectrochemistry

      Volume: 81 Issue: 1 Pages: 28-33

    • DOI

      10.1016/j.bioelechem.2011.01.002

    • NAID

      120002970832

    • Related Report
      2011 Annual Research Report 2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of"thin-layer"and"effective medium"approximations in numerical simulation of dielectric properties of biological cell suspensions2010

    • Author(s)
      K. Asami
    • Journal Title

      Jpn. J. Applied Physics

      Volume: 49 Issue: 12R Pages: 127001-127001

    • DOI

      10.1143/jjap.49.127001

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dielectric coagulometry : A new approach to estimate venous thrombosis risk2010

    • Author(s)
      Y.Hayashi, Y.Katsumoto, S.Omori, A.Yasuda, K.Asami, M.Kaibara, I.Uchimura
    • Journal Title

      Anal.Chem.

      Volume: 82 Pages: 9769-9774

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of "thin-layer" and "effective medium" approximations in numerical simulation of dielectric spectra of biological cell suspensions2010

    • Author(s)
      K.Asami
    • Journal Title

      Jpn.J.Appl.Phys.

      Volume: 49

    • NAID

      40017420100

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dielectric inspection of erythrocytes2010

    • Author(s)
      Y.Hayashi, Y.Katsumoto, I.Oshige, S.Omori, A.Yasuda, K.Asami
    • Journal Title

      J.Non-Crystalline Solids 356

      Pages: 757-762

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of dielectric spectra of erythrocytes with various shapes2009

    • Author(s)
      K. Asami
    • Journal Title

      J. Phys

      Volume: 42 Issue: 13 Pages: 135503-135503

    • DOI

      10.1088/0022-3727/42/13/135503

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effects of erythrocyte deformability upon hematocrit assessed by the conductance method2009

    • Author(s)
      Y.Hayashi, Y.Katsumoto, I.Oshige, S.Omori, A.Yasuda, K.Asami
    • Journal Title

      Phys.Med.Biol. 54

      Pages: 2395-2405

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of dielectric spectra of erythrocytes with various shapes2009

    • Author(s)
      K.Asami
    • Journal Title

      J.Phys.D : Appl.Phys. 42

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 誘電スペクトロスコピーによるコロイド分散系の評価2011

    • Author(s)
      浅見耕司
    • Organizer
      コロイドおよび界面化学討論会
    • Place of Presentation
      京都京都大学吉田キャンパス
    • Year and Date
      2011-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 誘電スペクトロスコピーによるコロイド分散系の評価2011

    • Author(s)
      浅見耕司
    • Organizer
      コロイドおよび界面化学討論会
    • Place of Presentation
      京都(京都大学吉田キャンパス)
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 赤血球ゴーストの誘電分散の観測と解析2011

    • Author(s)
      浅見耕司
    • Organizer
      日本膜学会33回年会
    • Place of Presentation
      東京産業技術総合研究所臨海副都心センター別館
    • Year and Date
      2011-05-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 赤血球ゴーストの誘電分散の観測と解析2011

    • Author(s)
      浅見耕司
    • Organizer
      日本膜学会
    • Place of Presentation
      東京(産業技術総合研究所臨海副都心センター別館)
    • Year and Date
      2011-05-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低張処理により赤血球膜にできた穴の誘電分光法による観測2010

    • Author(s)
      浅見耕司
    • Organizer
      膜シンポジウム2010
    • Place of Presentation
      京都京都大学吉田キャンパス
    • Year and Date
      2010-11-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 低張処理により赤血球膜にできた穴の誘電分光法による観測2010

    • Author(s)
      浅見耕司
    • Organizer
      膜シンポジウム2010
    • Place of Presentation
      京都
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞の増殖とアポトーシスの誘電モニター2009

    • Author(s)
      浅見耕司、金子英雄
    • Organizer
      日本膜学会
    • Place of Presentation
      東京
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi