Novel assay for determination of baculovirus titer using host cell binding of enzyme-displayed baculovirus
| Project/Area Number |
21560805
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
GOTOH Takeshi 秋田大学, 大学院・工学資源学研究科, 教授 (10215494)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 生物機能工学 / 生体認識 / ウイルス / バイオテクノロジー / 昆虫細胞 / バキュロウイルス / 酵素反応 / リアルタイムPCR / ウイルス検出 / ウイルス力価 / 酵素展示 |
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| Research Abstract |
The present study aimed at developing a novel assay method using a baculovirus (BV)-host cell binding for the determination of BV titer. First, we constructed recombinant BV displaying either GST or EGFP on GP64 envelope protein in order to distinguish between infected and uninfected cells. However, the protein probes displayed on BVs were not sensitive enough to use for this purpose. In addition, it was so difficult to produce the recombinant BVs because of their high susceptibility to endogenous proteases. Second, we examined the BV-host cell binding conditions to separate active and inactive BVs. As a result, it was suggested that the BV-host cell binding was applicable to the BV titer determination by combining with real-time PCR.
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Report
(4 results)
Research Products
(8 results)
