| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
In this study we have attempted to control the differentiation of embryoid bodies(EBs) derived from pluripotent stem cells(ES and iPS cells) by functional compounds. The functional compounds hardly influenced the size of EBs. The generation efficiency of beating cardiac cells was low in the EBs formed under 0. 5%-and 10%-PL, and high in those formed under MPC. The expression levels of・-MHC(mesoderm marker) and TTR(endoderm marker) were high in the EBs formed under MPC and low in those formed under PL. However, the activity and pluripotency of the cells at passage stage greatly affected the EB formation. The heterogeneity in cell quality before EB formation made more difficult to properly evaluate the real effect of functional compounds on the EB formation. We reaffirmed the importance of controlling the quality of the cells before use on the experiments.
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