Development of the effective novel identification method of the proteins which accumulate uranium

• Project/Area Number: 21560878
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nuclear engineering
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: SAKAMOTO Fuminori 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 研究副主幹 (60391273)
• Co-Investigator(Kenkyū-buntansha): KOZAI Naofumi 独立行政法人日本原子力研究開発機構, 端基礎研究センター, 研究主幹 (80354877) ; 鈴木 義規 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 博士研究員 (20455281)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 核燃料サイクル / 酵母 / ウラン濃集 / タンパク質 / ウラン濃集タンパク質 / 非含硫アミノ酸

Research Abstract

We developed new technique to identify the protein which specifically accumulated uranium effectively. This method uses the detection system that combined mass spectrograph with liquid chromatography using a size exclusion column. We tried to identify the proteins which accumulate uranium using this method. As a result, we made clear that about 46 kilo Daltons of protein accumulated uranium. It was also suggested that the protein did not conclude the amino acid including sulfur(methionine and cysteine).

Research Products

• [Journal Article] Yeast Genes Involved in Uranium Tolerance and Uranium Accumulation : A Functional Screening Using the Nonessential Gene Deletion Collection (2012)
  • Author(s): Fuminori Sakamoto、Takuya Nankawa、Toshihiko Ohnukia、Tsutomu Fujii and Haruyuki Iefuji
  • Journal Title: Geomicrobiology Journal Volume: 29巻 Issue: 5 Pages: 470-476
  • DOI: 10.1080/01490451.2011.581330
  • Peer Reviewed
• [Journal Article] Yeast Genes Involved in Uranium Tolerance and Uranium Accumulation : A Functional Screening Using the Nonessential Gene Deletion Collection (2012)
  • Author(s): 坂本文徳, 他
  • Journal Title: Geomicrobiology Journal Volume: 29 Pages: 470-476
  • Peer Reviewed
• [Journal Article] オートラジオグラフィーを用いた福島第一原子力発電所起源の放射性降下物の局所的な分布解析 (2012)
  • Author(s): 坂本文徳、香西直文, 他
  • Journal Title: 日本原子力学会和文論文誌 Volume: 11 Pages: 1-7
  • NAID: 10030136505
  • Peer Reviewed
• [Journal Article] Accumulation of Co in Yeast Cells under Metabolically Active Condition-Implication for Role of Yeast in Migration of Radioactive Co- (2011)
  • Author(s): N. KOZAI, T. OHNUKI, F. SAKAMOTO, Y. SUZUKI, K. TANAKA, 他2名
  • Journal Title: J. Nucl. Sci. Technol Volume: 48 Issue: 8 Pages: 1206-1213
  • DOI: 10.1080/18811248.2011.9711808
  • NAID: 10029130475
  • Peer Reviewed
• [Journal Article] Accumulation of Co n Yeast Cells under Metabolically Active condition-Implication for Role of Yeast in Migration of Radioactive Co- (2011)
  • Author(s): 香西直文、坂本文徳, 他
  • Journal Title: Journal of Nuclear Science and Technology Volume: 48 Pages: 1206-1213
  • Peer Reviewed
• [Journal Article] Behavior of Paramecium sp.in solutions containing Sr and Pb : Do Paramecium sp.alter chemical forms of those metals? (2011)
  • Author(s): Naofumi Kozai
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Volume: (in press)
  • Peer Reviewed
• [Journal Article] Yeast genes involved in uranium tolerance and uranium accumulation : A functional screening using the nonessential gene deletion collection (2011)
  • Author(s): Fuminori Sakamoto
  • Journal Title: Geomicrobiology Journal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Association of actinides with microorganisms and clay : Implications for radionuclide migration from waste-repository sites (2010)
  • Author(s): T. Ohnuki, N. Kozai, F. Sakamoto, T. Ozaki, T. Nankawa, Y. Suzuki, A. J. Francis
  • Journal Title: Geomicrobiology J Volume: 27 Issue: 3 Pages: 225-230
  • DOI: 10.1080/01490450903456715
  • Peer Reviewed
• [Journal Article] Eigo Kamiishi and Satoshi Utsunomiya、Biological nanomineralization of Ce phosphate by Saccharomyces cerevisiae (2010)
  • Author(s): Mingyu Jiang、Toshihiko Ohnuki、Naofumi Kozai、Kazuya Tanaka、Yoshinori Suzuki、Fuminori Sakamoto
  • Journal Title: Chemical Geology Volume: 277巻 Pages: 61-69
  • URL: http://dx.doi.org/10.1016/j.chemgeo.2010.07.010
  • Peer Reviewed
• [Journal Article] Response of Saccharomyces cerevisiae to heavy element stress : lead vs (2010)
  • Author(s): F. Sakamoto、T. Ohnuki、F. Fujii、and H. Iefuji
  • Journal Title: Geomicrobiology Journal Volume: 27巻 Issue: 3 Pages: 240-244
  • DOI: 10.1080/01490450903456756
  • Peer Reviewed
• [Journal Article] Response of Saccharomyces cerevisiae to Heavy Element Stress : Lead vs.Uranium (2010)
  • Author(s): Fuminori Sakamoto
  • Journal Title: Geomicrobiology Journal Volume: 27 Pages: 240-244
  • Peer Reviewed
• [Journal Article] Association of actinides with microorganisms and clay : Implications for radionuclide migration from waste-repository sites (2010)
  • Author(s): Naofumi Kozai
  • Journal Title: Geomicrobiology Journal Volume: 27 Pages: 225-230
  • Peer Reviewed
• [Journal Article] Response of Saccharomyces cerevisiae to Heavy Element Stress : Lead vs. Uranium (2010)
  • Author(s): Fuminori Sakamoto
  • Journal Title: Geomicrobiology Journal 27 Pages: 240-244
  • Peer Reviewed
• [Journal Article] Effect of citrate, NTA, and EDTA on the reduction of U(VI) by Shewanella putrefaciens (2010)
  • Author(s): Yoshinori Suzuki
  • Journal Title: Geomicrobiology Journal 27 Pages: 245-250
  • Peer Reviewed
• [Journal Article] Association of actinides with microorganisms and clay : Implications for radionuclide migration from waste-repository sites (2010)
  • Author(s): Naofumi Kozai, Yoshinori, Suzuki
  • Journal Title: Geomicrobiology Journal 27 Pages: 225-230
  • Peer Reviewed
• [Journal Article] In situ observation of reductive deposition of uranium on an electrode/electrolyte interface by optical waveguide spectroscopy (2009)
  • Author(s): Yoshinori Suzuki
  • Journal Title: Chemistry Letters 38 Pages: 1090-1091
  • NAID: 10025548262
  • Peer Reviewed
• [Journal Article] Sorption and oxidation of tetravalent plutonium on Mn oxide in the presence of citric acid (2009)
  • Author(s): Yoshinori Suzuki
  • Journal Title: Chemistry Letters 38 Pages: 1032-1033
  • NAID: 10025547731
  • Peer Reviewed
• [Journal Article] Modeling of interaction of Pu(VI) with the mixture of microorganism and clay (2009)
  • Author(s): Yoshinori Suzuki
  • Journal Title: Journal of Nuclear Science and Technology 46 Pages: 55-59
  • NAID: 10024656488
  • Peer Reviewed
• [Presentation] ウラン存在下で発現する酵素タンパク質の同定 (2011)
  • Author(s): 坂本文徳
  • Organizer: 第13回放射性廃棄物地層処分に関する情報交換会
  • Place of Presentation: 札幌
• [Presentation] ガンマ線照射によるDNA切断の一塩基対ごとの位置特定 (2010)
  • Author(s): 坂本文徳
  • Organizer: 日本放射線影響学会第53回退会
  • Place of Presentation: 京都
• [Presentation] ウラン耐性とウラン濃集に関わる酵母遺伝子の探索 (2009)
  • Author(s): 坂本文徳
  • Organizer: 日本生化学会2009年年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 重金属ストレスに対する酵母の応答:鉛とウランの比較 (2009)
  • Author(s): 坂本文徳
  • Organizer: 日本地球化学会2009年年会
  • Place of Presentation: 広島
  • Year and Date: 2009-09-15
• [Presentation] Effects of organic acids on microbial reduction of U(VI) (2009)
  • Author(s): Yoshinori Suzuki
  • Organizer: The 238th ACS National Meeting, NUCL77
  • Place of Presentation: Washington, USA
• [Presentation] Biomineralization of REE phosphate minerals by Saccharomyces cerevisiae (2009)
  • Author(s): Yoshinori Suzuki
  • Organizer: Migration 09
  • Place of Presentation: Washington, USA
• [Presentation] Flavin mononucleotide mediated reduction of U(VI) (2009)
  • Author(s): Yoshinori Suzuki
  • Organizer: Goldschmidt 2009
  • Place of Presentation: Davos, Switzerland
• [Presentation] Effect of organic acids on interaction of actinides with microorganisms (2009)
  • Author(s): Yoshinori Suzuki
  • Organizer: Goldschmidt 2009
  • Place of Presentation: Davos, Switzerland
• [Presentation] クエン酸,NTAおよびEDTA存在下におけるU(VI)の微生物還元挙動 (2009)
  • Author(s): 鈴木義規
  • Organizer: 日本地球化学会第56回年会
  • Place of Presentation: 広島
• [Presentation] U(VI)の微生物還元におけるフラビンモノヌクレオチドの役割 (2009)
  • Author(s): 鈴木義規
  • Organizer: 日本原子力学会2009年秋の大会
  • Place of Presentation: 仙台