| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Budding yeast Cdc3 is a component of septin rings which localize at the neck region andis essential for cytokinesis. Cdc3 was transiently sumoylated by Siz1, a SUMO ligase onlyduring M-phase. Cdc3 was ubiquitinated in Rsp5-dependent way, but the PY-motif of Cdc3was not required for the ubiquitination. We examined the phenotypes of various mutantswhen Siz1-septin or SUMO-septin fusion proteins were over-expressed. Results suggestedthat the septin sumoylation induces the depolymerization of septin rings, and that thesumolylated Siz1 was ubiquitinated by Slx8/Slx5 ubiquitin ligase for its degradation.
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