Cell and signal mechanisms that support tissue regeneration in fish

• Project/Area Number: 21570004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KAWAKAMI Atsushi 東京工業大学, 大学院・生命理工学研究科, 准教授 (00221896)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: モデル生物 / 小型魚類における組織再生 / ゼブラフィッシュ / トランスジェニ / Junb / JNK / 再生 / 小型魚類 / 細胞死 / トランスジェニック / 組織ホメオスタシス / シグナル伝達

Research Abstract

To clarify the tissue homeostasis mechanism of multicellular organisms, we analyzed the regeneration processes of adult and larval zebrafish tissues and obtained the following results.
1. The signaling pathway involving the Junb, Junbl and JNK has an essential role in regeneration.
2. To reveal the origin and fate of regenerating cells, we generated transgenic lines for cell fate tracing and cell ablation.
3. From the analysis of regeneration defective zebrafish mutant, we showed that a factor derived from the hematopoietic tissues is necessary for the survival of regenerating cells.

Research Products

• [Journal Article] Filamin C plays an essential role in the maintenance of the structural integrity of cardiac and skeletal muscles, revealed by the medaka mutant zacro (2012)
  • Author(s): Fujita, M., Mitsuhashi, H., Isogai, S., Nakata, T., Kawakami, A., Nonaka, I., Noguchi, S., Hayashi, Y. K., Nishino, I. and Kudo, A
  • Journal Title: Developmental Biology Volume: 361 Pages: 79-89
  • Peer Reviewed
• [Journal Article] Mature and Juvenile Tissue Models of Regeneration in Small Fish Species (2011)
  • Author(s): Yoshinari, N. and Kawakami, A
  • Journal Title: Biological Bulletin Volume: 221 Pages: 62-78
  • Peer Reviewed
• [Journal Article] Mature and Juvenile Tissue Models of Regeneration in Small Fish Species (2011)
  • Author(s): Yoshinari, N., Kawakami, A.
  • Journal Title: Biological Bulletin Volume: 221 Pages: 62-68
  • Peer Reviewed
• [Journal Article] Mature and Juvenile Tissue Models of Regeneration in Small Fish Species. (2011)
  • Author(s): Yoshinari, N., Kawakami, A.
  • Journal Title: Biological Bulletin Volume: (in press)
  • Peer Reviewed
• [Journal Article] Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebra fish (2010)
  • Author(s): Ishida, T., Nakajima, T., Kudo, A. and Kawakami, A
  • Journal Title: Developmental Biology Volume: 340 Pages: 468-479
• [Journal Article] Epigenetic control mechanism of cardiomyocyte production in response to a stress during the medaka heart development (2010)
  • Author(s): Taneda, Y., Konno, S., Makino, S., Fukuda, K., Imai, Y., Kudo, A. and Kawakami, A.
  • Journal Title: Developmental Biology Volume: 340 Pages: 30-40
• [Journal Article] Stem cell system in tissue regeneration in fish (2010)
  • Author(s): Kawakami, A
  • Journal Title: Development Growth & Differentiation Volume: 52 Pages: 77-87
  • NAID: 10027563168
  • Peer Reviewed
• [Journal Article] 「増殖か死か」:組織再生における増殖細胞の生存戦略。 (2010)
  • Author(s): 川上厚志
  • Journal Title: 実験医学(4月増刊) Volume: 28 Pages: 159-166
• [Journal Article] Stem cell system in tissue regeneration in fish. (2010)
  • Author(s): Kawakami, A.
  • Journal Title: Development Growth & Differentiation 52 Pages: 77-87
  • NAID: 10027563168
  • Peer Reviewed
• [Journal Article] Epigenetic control mechanism of cardiomyocyte production in response to a stress during the medaka heart development. (2010)
  • Author(s): Taneda, Y., Konno, S., Makino, S., Fukuda, K., Imai, Y., Kudo, A., Kawakami, A.
  • Journal Title: Developmental Biology 340 Pages: 30-40
  • Peer Reviewed
• [Journal Article] Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish (2010)
  • Author(s): Ishida, T., Nakajima, T., Kudo, A., Kawakami, A.
  • Journal Title: Develepmental Biology (In press)
  • Peer Reviewed
• [Journal Article] Gene expression and functional analysis of zebra fish larval fin fold regeneration (2009)
  • Author(s): Yoshinari, N., Ishida, T., Kudo, A., and Kawakami, A
  • Journal Title: Developmental Biology Volume: 325 Pages: 71-81
• [Presentation] 魚類における2つのフィブロネクチンの再生における働きの解析 (2011)
  • Author(s): 村瀬瑛美子、工藤明、川上厚志
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 村瀬瑛美子、工藤明、川上厚志 (2011)
  • Author(s): 村瀬瑛美子、工藤明、川上厚志
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Cell ablation analysis in the regenerating zebrafish fin : Generation of transgenic zebrafish and the cell ablation using the prodrug and nitroreductaseゼブラフィッシュ再生ひれにおける細胞除去:トランスジェニック作製とプロドラッグ、ニトロリダクターゼを用いた細胞除去 (2011)
  • Author(s): 安藤和則、吉成望、工藤明、川上厚志
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] 安藤和則、吉成望、工藤明、川上厚志 (2011)
  • Author(s): 安藤和則、吉成望、工藤明、川上厚志
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Amechanism that controls blastema cell proliferation and survival during zebrafish fin fold regenerationゼブラフィッシュ膜ひれ再生における再生芽の増殖と生存の調節 (2011)
  • Author(s): 川上厚志、中島輝弘、石田高志、工藤明
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] 川上厚志、中島輝弘、石田高志、工藤明 (2011)
  • Author(s): 川上厚志、中島輝弘、石田高志、工藤明
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Amechanism that controls blastema cell proliferation and survival during fin fold regeneration (2011)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Atsushi Kawakami.
  • Organizer: The21st CDB Meeting "From Regeneration Biology to Regenerative Medicine(I)"
  • Place of Presentation: RIKEN CDB, Kobe
• [Presentation] Towards understanding tissue regeneration and homeostasis mechanism using fish regeneration models (2011)
  • Author(s): Atsushi Kawakami, Natsumi Horita, Tomomi Kushida, Kazunori Ando, EmikoMurase, Nozomi Yoshianri, Masato Kinoshita, Genbu Abe, Koichi Kawakami, and Tohru Ishitani
  • Organizer: The21st CDB Meeting "From Regeneration Biology to Regenerative Medicine(I)"
  • Place of Presentation: RIKEN CDB, Kobe
• [Presentation] Atsushi Kawakami, Natsumi Horita, Tomomi Kushida, Kazunori Ando, Emiko Murase, Nozomi Yoshianri, Masato Kinoshita, Genbu Abe, Koichi Kawakami, Tohru Ishitani (2011)
  • Author(s): Atsushi Kawakami. Natsumi Horita, Tomomi Kushida, Kazunori Ando, Emiko Murase, Nozomi Yoshianri, Masato Kinoshita, Genbu Abe, Koichi Kawakami, Tohru Ishitani
  • Organizer: The21st CDB Meeting" From Regeneration Biology to Regenerative Medicine (I)
  • Place of Presentation: 神戸
• [Presentation] Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Atsushi Kawakami (2011)
  • Author(s): Tomova Hasegawa, Te akajima, Takashi Ishida, Atsushi Kawakami
  • Organizer: The21st CDB Meeting" From Regeneration Biology to Regenerative Medicine (I)
  • Place of Presentation: 神戸
• [Presentation] ユビキタスに蛍光タンパクを発現する新たなゼブラフィッシュ系統 (2010)
  • Author(s): 吉成望、工藤明、川上厚志
  • Organizer: 第10回小型魚類研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-09-18
• [Presentation] 心筋・骨格筋に異常を示すメダカ変異体zacroの解析 (2010)
  • Author(s): 三橋弘明、藤田深里、安田裕隆、林由起子、野口悟、埜中征哉、川上厚志、工藤明、西野一三
  • Organizer: 第10回小型魚類研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-09-18
• [Presentation] 心筋・骨格筋に異常を示すメダカ変異体zacroの解析 (2010)
  • Author(s): ^○三橋弘明、藤田深里、安田裕隆、林由起子、野口悟、埜中征哉、川上厚志、工藤明、西野一三
  • Organizer: 第10回 小型魚類研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-09-18
• [Presentation] ユビキタスに蛍光タンパクを発現する新たなゼブラフィッシュ系統 (2010)
  • Author(s): 吉成望、工藤明、^○川上厚志
  • Organizer: 第10回 小型魚類研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-09-18
• [Presentation] Checkpoint for fast proliferating blastema cells during finfold regeneration,再生細胞の早い細胞増殖を検問するチェックポイントメカニズム (2010)
  • Author(s): Teruhiro Nakajima, Akira Kudo, and Atsushi Kawakami
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Cell lineage analysis of fish fin blastema for their potential producing multiple cell types during regeneration再生芽細胞の多分化能力を検証するための、魚類鰭の再生組織における細胞系譜の解析,再生細胞の早い細胞増殖を検問するチェックポイントメカニズム (2010)
  • Author(s): Nozomi Yoshinari, Akira Kudo, OAtsushiKawakami
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Checkpoint for fast proliferating blastema cells during finfold regeneration (2010)
  • Author(s): Teruhiro Nakajima, Akira Kudo, Atsushi Kawakami
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Cell lineage analysis of fish fin blastema for their potential producing multiple cell types during regeneration (2010)
  • Author(s): Nozomi Yoshinari, Akira Kudo, ^○Atsushi Kawakami
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] ゼブラフィッシュにおける再生芽細胞の分化多能性の検証 (2009)
  • Author(s): 吉成望、工藤明、川上厚志
  • Organizer: 第31回日本分子生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Indispensable role of Junb/Jnk signaling in regeneration : regeneration can be demarcated from wound healing by the dependence on Junb/Jnk signaling (2009)
  • Author(s): Takashi Ishida, Akira Kudo, and OAtsushi Kawakami
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-30
• [Presentation] cloche mutation causes the blastema apoptosis during the zebrafish fin fold regeneration (2009)
  • Author(s): Teruhiro Nakajima, Takashi Ishida, Akira Kudo, Atsushi Kawakami
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-30
• [Presentation] Indispensable role of Junb/Jnk signaling in regeneration : regeneration can be demarcated from wound healing by the dependence on Junb/Jnk signaling (2009)
  • Author(s): Takashi Ishida, Akira Kudo, *Atsushi Kawakami
  • Organizer: 第41回可本発生生物学会大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-30
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