Understanding the novel interplay between telomere and helicase in chromosome maintenance

• Project/Area Number: 21570006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Hiroshima University
• Principal Investigator: MASARU Ueno 広島大学, 大学院・先端物質科学研究科, 准教授 (90293597)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: テロメア / 分裂酵母 / RecQヘリケース / 微小管重合阻害剤 / 染色体分配 / ヘリケース / DNA組換え

Research Abstract

The double mutants between fission yeast telomere binding protein Pot1 and Rqh1 helicase and the double mutant between fission yeast telomerase Trt1 and Rqh1 helicase are sensitive to microtubule drug TBZ. We found that the reason for the TBZ sensitivity is due to the accumulation of recombination intermediates at telomere in M phase, which inhibits chromosome segregation.

Research Products

• [Journal Article] Double mutant between fission yeast telomerase and RecQ helicase is sensitive to anti-microtubule drug (2012)
  • Author(s): S. Ukimori, N. Kawabata, H. Shimada, Ryota Imano, K. Takahashi, M. Yukawa, E. Tsuchiya, and M. Ueno
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 76 Pages: 264-269
  • Peer Reviewed
• [Journal Article] A Double Mutant between Fission Yeast Telomerase and RecQ Helicase Is Sensitive to Thiabendazole, an Anti-Microtubule Drug (2012)
  • Author(s): S.Ukimori, N.Kawabata, H.Shimada, Ryota Imano, K.Takahashi, M.Yukawa, E.Tsuchiya, M.Ueno
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 264-269
  • DOI: 10.1271/bbb.110606
  • NAID: 10030399595
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation (2011)
  • Author(s): K. Takahashi, R. Imano, T. Kibe, H. Seimiya, Y. Muramatsu, N. Kawabata, G. Tanaka, Y. Matsumoto, T. Hiromoto, Y. Koizumi, N. Nakazawa, M. Yanagida, M. Yukawa, E. Tsuchiya, M. Ueno
  • Journal Title: Mol. Cell. Biol Volume: 31 Pages: 495-506
  • NAID: 120004205356
  • Peer Reviewed
• [Journal Article] Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation (2011)
  • Author(s): 高橋克典, ら
  • Journal Title: Mol.Cell.Biol. Volume: 31 Pages: 495-506
  • Peer Reviewed
• [Journal Article] Expression of Mutant RPA in Human Cancer Cells Causes Telomere Shortening (2010)
  • Author(s): Y. Kobayashi, K. Sato, T. Kibe, H. Seimiya, A. Nakamura, M. Yukawa, E. Tsuchiya, and M. Ueno
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 382-385
  • NAID: 10027551727
  • Peer Reviewed
• [Journal Article] Roles of DNA repair proteins in telomere maintenance (2010)
  • Author(s): M. Ueno Biosci
  • Journal Title: Biotechnol. Biochem Volume: 74 Pages: 1-6
  • NAID: 10027549715
  • Peer Reviewed
• [Journal Article] Roles of DNA repair proteins in telomere maintenance (2010)
  • Author(s): 上野勝
  • Journal Title: Biosci Biotechnol Biochem 1 Pages: 1-6
  • NAID: 10027549715
  • Peer Reviewed
• [Journal Article] Expression of mutant RPA in human cancer cells causes telomere shortening (2010)
  • Author(s): 小林由佳
  • Journal Title: Biosci Biotechnol Biochem 2 Pages: 382-385
  • Peer Reviewed
• [Presentation] テロメアの保護は正常な染色体分配に必要である (2011)
  • Author(s): 上野勝
  • Organizer: 第34回日本分子生物学会ワークショップDNAダイナミクスによる染色体維持の新展開の企画
  • Place of Presentation: 横浜(口頭発表)(招待講演)
  • Year and Date: 2011-12-15
• [Presentation] Dysfunctional telomere activates both DNA damage checkpoint and spindle checkpoint in fission yeast S.pombe (2011)
  • Author(s): 上野勝
  • Organizer: The 5th International Workshop on Cell Regulations in Division & Arrest
  • Place of Presentation: Okinawa, Japan(招待講演)
  • Year and Date: 2011-10-26
• [Presentation] Entangled telomere activates both DNA damage checkpoint and spindle checkpoint (2011)
  • Author(s): 上野勝
  • Organizer: The 6th international fission yeast meeting
  • Place of Presentation: Boston, U.S.A.
  • Year and Date: 2011-06-28
• [Presentation] Roles of Fission Yeast Potl and RecQ Helicases in Telomere Protection (2011)
  • Author(s): 上野勝
  • Organizer: Cold Spring Haber Laboratory Meeting Telomere and Telomerase
  • Place of Presentation: New York, U.S.A.
  • Year and Date: 2011-05-04
• [Presentation] Dysfunctional telomere activatesboth DNA damage checkpoint andspindle checkpoint in fission yeast (2011)
  • Author(s): S. pombe M. Ueno
  • Organizer: The 5th International Workshop onCell Regulations in Division & Arrest
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2011-04-04
• [Presentation] Entangled telomere activates both DNA damage checkpoint and spindlecheckpoint (2011)
  • Author(s): M. Ueno
  • Organizer: The 6th international fission yeastmeeting
  • Place of Presentation: Boston, U. S. A.
  • Year and Date: 2011-01-14
• [Presentation] Roles of Fission Yeast Pot1 and RecQHelicases in Telomere Protection (2011)
  • Author(s): M. Ueno
  • Organizer: Cold Spring Haber Laboratory MeetingTelomere and Telomerase
  • Place of Presentation: New York, U. S. A.
  • Year and Date: 2011-01-05
• [Presentation] テロメアの保護は正常な染色体分配に必要である (2010)
  • Author(s): 上野勝
  • Organizer: BMB2010第33回 日本分子生物学会年会 第83回 日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(口頭発表 招待講演)
  • Year and Date: 2010-12-07
• [Presentation] Constant existence of recombination intermediatesat chromosome ends impedes chromosome segregation (2010)
  • Author(s): 上野勝
  • Organizer: 3R symposium国際学会
  • Place of Presentation: 富山国際会議場(ポスター発表)
  • Year and Date: 2010-10-28
• [Presentation] Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation (2010)
  • Author(s): 上野勝
  • Organizer: EMBO conference series Telomeres and the DNA Damage Response
  • Place of Presentation: フランス マルセイユ(ポスター発表)
  • Year and Date: 2010-09-15
• [Presentation] Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation (2010)
  • Author(s): 上野勝
  • Organizer: FASEB Summer research conference yeast chromosome structure, replication and segregation
  • Place of Presentation: 米国、アリゾナ(ポスター発表)
  • Year and Date: 2010-08-09
• [Presentation] Telomere binding protein and RecQ helicase are required for chromosome segregation (2009)
  • Author(s): 上野勝
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 分裂酵母テロメア関連因子とRecQヘリケースの染色体維持における役割 (2009)
  • Author(s): 上野勝
  • Organizer: 酵母遺伝学フォーラム 口頭発表
  • Place of Presentation: 筑波、ノバホール
  • Year and Date: 2009-07-28
• [Presentation] RecQ helicase at dysfunctional telomere (2009)
  • Author(s): 上野勝
  • Organizer: Cold Spring Haber Laboratory Meeting Telomere and Telomerase
  • Place of Presentation: アメリカ、ニューヨーク
  • Year and Date: 2009-05-02
• [Presentation] Novel Roles of Telomere Proteins and Rec Q Helicases in Genome Stability (2009)
  • Author(s): M. Ueno
  • Organizer: The 5th international fission yeastmeeting
  • Place of Presentation: Tolyo, Japan
  • Year and Date: 2009-03-25
• [Presentation] Roles of Pot1 and RecQ Helicases in Genome Stability (2009)
  • Author(s): M. Ueno
  • Organizer: Switzerland-Japan Meeting on the Molecular Mechanisms Regulating Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars, Switzerland
  • Year and Date: 2009-03-08
• [Presentation] Role of RecQ Helicase at Dysfunctional Telomere (2009)
  • Author(s): M. Ueno
  • Organizer: Cold Spring Haber Laboratory Meeting Telomere and Telomerase
  • Place of Presentation: New York, U. S. A.
• [Book] Roles of DNA Repair Proteins in TelomereMaintenance (2011)
  • Author(s): タイトルDNA Repair
  • Publisher: InTech, ISBN 978-953-307-697-3編集InnaKruman 2011
• [Book] DNA Repair編集Inna Kruman (2011)
  • Author(s): Masaru Ueno
  • Total Pages: 15
  • Publisher: InTech