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Analysis of RagA, B/C, D in mTOR signal transduction system

Research Project

Project/Area Number 21570007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionKyushu University

Principal Investigator

SEKIGUCHI Takeshi  九州大学, 医学研究院, 助教 (60187846)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords分子遺伝 / 遺伝学 / 遺伝子 / 環境 / 微生物 / シグナル伝達
Research Abstract

The objective of this research was to find when, where, and how the G proteins(RagA, B/C, D) regulate mTOR signal transduction pathway. Moreover, we tried to identify roles of interacting proteins of the G proteins in mTOR pathway.
We elucidated significance of Gtrlp and Gtr2p complex formation and interaction to other proteins(Ego3 and Egol) by using G protein mutant proteins.
We found that Gtrlp interacted to Torl complex proteins Kogl and Tco89 in a specific manner. Interaction between Gtrlp and Tco89p was important.
We showed that human RagA, C regulate mTOR and AMPK. It was shown that AMPK regulates mTOR. Here we found that RagA, C could be bridge proteins for mTOR and AMPK.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (16 results) Remarks (5 results)

  • [Journal Article] 2011 Ubiquitin chains in the DSK2 UBL domain mediate DSK2 stability and protein degradation in Yeast2011

    • Author(s)
      Sekiguchi, T., Sasaki, T., Funakoshi, M., Ishii, T., Saitoh, Y., Kaneko, S., Kobayashi, H.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 411 Pages: 555-561

    • Related Report
      2011 Final Research Report
  • [Journal Article] Ubiquitin chains in the DSK2 UBL domain mediate DSK2 stability and protein degradation in Yeast2011

    • Author(s)
      Sekiguchi, et al
    • Journal Title

      Biochem.Biophys.Res.Commun

      Volume: 411 Pages: 555-561

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Gtr1p associated with Gtr2p and with Ego1p differently2009

    • Author(s)
      Wang, et al.
    • Journal Title

      Gene 437

      Pages: 32-38

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2010 New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi

    • Author(s)
      ekiguchi T, Sasaki H, Kurihara Y, Watanabe S, Moriyama D, Kurose N, Matsuki R, Yamazaki K, Saeki M.
    • Journal Title

      Mustela sibirica, and Martes me/ampus Mol. Eco. Res

      Volume: 10 Pages: 1089-1091

    • Related Report
      2011 Final Research Report
  • [Journal Article] 2009 The Gtr 1 p associated with Gtr2p and with Egol p differently

    • Author(s)
      Wang, YG., Kurihara, Y., Sato, T., Toh, H., Kobayashi, H. and Sekiguchi, T.
    • Journal Title

      Gene

      Volume: 437 Pages: 32-38

    • Related Report
      2011 Final Research Report
  • [Journal Article] 2009 Ran GTPase guanine nucleotide exchange factor

    • Author(s)
      Horiike, Y, Kobayashi, H., Sekiguchi, T.
    • Journal Title

      RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro Mo I. Biol. Rep

      Volume: 36 Pages: 717-723

    • Related Report
      2011 Final Research Report
  • [Presentation] ヘテロ2量体ヘテロ2量体ヘテロ2量体ヘテロ2量体ヘテロ2量体Gタンパク質のGtr1, Gtr2とTor複合体1との相互作用2011

    • Author(s)
      関口猛、鎌田芳彰、古野伸明、小林英紀
    • Organizer
      日本遺伝学会第83回大会
    • Year and Date
      2011-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] ヘテロ2量体Gタンパク質のGtr1、Gtr2とTor複合体1との相互作用2011

    • Author(s)
      関口猛
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都大学、京都市(招待講演)
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Torシグナル伝達系のヘテロ2量体Gtr1ーGtr2のGtr1とGtr2の働きの違いの解析2010

    • Author(s)
      関口猛、舟越稔、古野伸明、小林英紀,関口猛、舟越稔、古野伸明、小林英紀
    • Organizer
      第33回目本分子生物学会年会
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Torシグナル伝達系のヘテロ2量体Gtr1-Gtr2のGtr1とGtr2の働きの違いの解析2010

    • Author(s)
      関口猛
    • Organizer
      第33回日本分子生物学会年会(BMB2010)
    • Place of Presentation
      神戸ポートアイランド、神戸市
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Interaction of AMPKgamma to Nop132, a nucleolar protein2010

    • Author(s)
      関口猛
    • Organizer
      Faseb summer research meeting, AMPK : Central Regulatory System in Metabolizm & Growth
    • Place of Presentation
      大津プリンスホテル、大津市
    • Year and Date
      2010-10-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Interaction of AMPK gamma to Nop 132, a nucleolar protein2010

    • Author(s)
      Takeshi Sekiguchi
    • Organizer
      FASEB summer research meeting
    • Year and Date
      2010-10-04
    • Related Report
      2011 Final Research Report
  • [Presentation] ヘテロ2量体Gタンパク質のGtrl, Gtr2は単独で相互作用タンパク質Egol, Ego3に結合する2010

    • Author(s)
      関口猛、舟越稔、古野伸明、小林英紀
    • Organizer
      日本遺伝学会第82回大会
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] ヘテロ2量体Gタンパク質のGtr1,Gtr2は単独で相互作用タンパク質Ego3,Ego1と結合する2010

    • Author(s)
      関口猛
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      北海道大学、札幌市
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hideki Kobayashi, Role of Gtrl-Gtr2 complex formation on their biologic function2010

    • Author(s)
      Takeshi Sekiguchi, Yoshiaki Kamada, Yoshinori Ohsumi, Yonggang Wang
    • Organizer
      The 24th annual symposium of the protein society
    • Year and Date
      2010-08-03
    • Related Report
      2011 Final Research Report
  • [Presentation] Role of Gtr1-Gtr2 complex formation on their biologic function.2010

    • Author(s)
      関口猛
    • Organizer
      The 24th annual symposium of The Protein Society
    • Place of Presentation
      マンチェスターグランドハイアット、サンディエゴ、米国
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Gtrl-Gtr 2複合体のTORングナル経路における機能解析2009

    • Author(s)
      関口猛、鎌田芳彰、大隅良典、王永剛、小林英紀
    • Organizer
      第32回日本分子生物学会
    • Year and Date
      2009-12-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Gtr1-Gtr2複合体のTORシグナル経路における機能解析2009

    • Author(s)
      関口猛
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Gtr 2Gtr1複合体形成にロイシンジッパーモチーフが必須である2009

    • Author(s)
      関口猛、王永剛、小林英紀
    • Organizer
      日本遺伝学会第81回大会
    • Year and Date
      2009-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Gtr1-Gtr2複合体形成にロイシンジッパーモチーフが必須である2009

    • Author(s)
      関口猛
    • Organizer
      日本遺伝学会第81回大会
    • Place of Presentation
      信州大学, 松本市
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of Gtr1/2 small G proteins2009

    • Author(s)
      Takeshi Sekiguchi, Yonggang Wang, Naoyuki Hayashi, Hiroyuki Toh, Hideki Kobayashi
    • Organizer
      The 23rd annual symposium of The Protein Society
    • Year and Date
      2009-07-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of Gtr1/2 small G proteins2009

    • Author(s)
      関口猛
    • Organizer
      The 23rd annual symposium of The Protein Society
    • Place of Presentation
      マリオットホテル、ボストン、米国
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://gtrlgtr2.blog.ocn.ne.jp/blog/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000857/research.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://gtrlgtr2.blog.ocn.ne.jp/blog/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000857/research.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000857/research.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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