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Establishment and erasure of genomic imprinting during somatic and germline differentiation

Research Project

Project/Area Number 21570009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionJichi Medical University (2010-2011)
The Institute of Physical and Chemical Research (2009)

Principal Investigator

MISE Nathan  自治医科大学, 医学部, 助教 (00360644)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsゲノム刷り込み / DNAメチル化 / ES細胞 / 試験管内分化系 / 遺伝子発現 / エピジェネティクス / 細胞分化 / 生殖細胞分化 / ヒストン修飾 / EG細胞 / SNPs
Research Abstract

Genomic imprinting is a gene regulatory mechanism in which genes only derived from one parent to work. Genomic imprinting is known to be regulated by DNA methylation, but detail molecular mechanisms establishing parent-of-origin dependent gene expression pattern is still unclear. In this study, we showed that the expression pattern of imprinted gene is established accompanying cell differentiation using with an ES cell in vitro differentiation system. We further established a in vitro cell culture system to analyze detailed molecular mechanisms of establishment of imprinted expression pattern of imprinted genes.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (11 results)

  • [Journal Article] Incomplete X-inactivation initiated by a hypomorphic Xist allele in the mouse2011

    • Author(s)
      Hoki, Y., et al
    • Journal Title

      Development

      Volume: 138 Issue: 13 Pages: 2649-2659

    • DOI

      10.1242/dev.061226

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] LINE-1 Activity in Facultative Heterochromatin Formation during X Chromosome Inactivation2010

    • Author(s)
      Chow J. C., Ciaudo C., Fazzari M. J., Mise N., Servant N., Glass J. L., Attreed M., Avner P., Wutz A., Barillot E., Greally J. M., Voinnet O., Heard E
    • Journal Title

      Cell

      Volume: 141 Pages: 956-969

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes2010

    • Author(s)
      Imamura M. Aoi T., Tokumasu A., Mise N., Abe K., Yamanaka S., and Noce T
    • Journal Title

      Mol. Reprod

      Volume: 77 Pages: 802-811

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos2010

    • Author(s)
      Kobayashi S., Fujihara Y., Mise N., Kaseda K., Abe K., Ishino F., and Okabe M
    • Journal Title

      Nucleic Acids Research

      Volume: 38 Pages: 3672-3681

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 刷り込み型遺伝子発現におけるDNAメチル化の影響:ES細胞を用いた解析2010

    • Author(s)
      三瀬名丹
    • Journal Title

      Japanese Journal of Clinical Ecology

      Volume: 19 Pages: 72-78

    • NAID

      10029424011

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LINE-1 Activity in Facultative Heterochromation Formation during X chromosome Inactivation2010

    • Author(s)
      Chow, et al., (13人中4番目)
    • Journal Title

      Cell

      Volume: 141 Pages: 956-969

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes.2010

    • Author(s)
      Imamura, et al., (7人中4番目)
    • Journal Title

      Molecular Reproduction and Development

      Volume: 77 Pages: 3672-3681

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The X-linked imprinted gene family Fthl17 shows predominantoly female expression following the two-cell stage in mouse embryos2010

    • Author(s)
      Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M.
    • Journal Title

      Nucleic Acids Res (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A high-speed congenic strategy using first-wave male germ cells2009

    • Author(s)
      Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
    • Journal Title

      PLoS ONE 4

      Volume: 4

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A high-speed congenic strategy using first-wave male germ cells2009

    • Author(s)
      Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A.
    • Journal Title

      PLoS ONE 4(3)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Embryonic stem cell test(EST): toward epigenetic analyses for fetal exposure to environmental pollutants2011

    • Author(s)
      三瀬名丹、香山不二雄
    • Organizer
      日本分子生物学会第34回年会
    • Place of Presentation
      東京都・文京区
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Embryonic stem cell test (EST) : toward epigenetic analyses for fetal exposure to environmental pollutants2011

    • Author(s)
      三瀬名丹、香山不二雄
    • Organizer
      日本分子生物学会第33回年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 環境汚染物質の胎児期暴露評価・解析系としてのES細胞2011

    • Author(s)
      三瀬名丹、香山不二雄
    • Organizer
      環境ホルモン学会第14回研究発表会
    • Place of Presentation
      東京都・文京区
    • Year and Date
      2011-12-01
    • Related Report
      2011 Final Research Report
  • [Presentation] 環境汚染物質の胎児期曝露評価・解析系としてのES細胞2011

    • Author(s)
      三瀬名丹、香山不二雄
    • Organizer
      日本内分泌撹乱物質学会第14回研究発表会
    • Place of Presentation
      東京大学山上会館
    • Year and Date
      2011-12-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウスIgf2r遺伝子の刷り込み型遺伝子発現の成立に関わるDNAメチル化のES細胞を用いた解析2010

    • Author(s)
      三瀬名丹、近藤昌代、阿部訓也
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      北海道・札幌
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] マウスIgf2遺伝子の刷り込み型発現制御の成立に関わるDNAメチル化のES細胞を用いた解析2010

    • Author(s)
      三瀬名丹、近藤昌代、阿部訓也
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      北海道大学・機能開発総合センター
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 刷り込み型遺伝子発現におけるDNAメチル化の影響: ES細胞を用いた解析2010

    • Author(s)
      三瀬名丹
    • Organizer
      第19回日本臨床環境医学会学術集会シンポジウム
    • Place of Presentation
      東京都・港区
    • Year and Date
      2010-07-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 刷り込み型遺伝子発現におけるDNAメチル化の影響:ES細胞を用いた解析2010

    • Author(s)
      三瀬名丹
    • Organizer
      第19回日本臨床環境医学会学術集会シンポジウム
    • Place of Presentation
      北里大学・白金キャンパス
    • Year and Date
      2010-07-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] New in vitro model for analyzing impact of DNA methylation and demethylation to imprint genes2010

    • Author(s)
      三瀬名丹、近藤昌代、阿部訓也
    • Organizer
      Keystone Symposia(Developmental Origins and Epigenesisin Human Health and Disease)
    • Place of Presentation
      Singapore
    • Year and Date
      2010-04-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of germline differentiation in vitro using mouse ES cell lines that express germline specific fluorescent reporter2009

    • Author(s)
      三瀬名丹、近藤昌代、池田理恵子、阿部訓也
    • Organizer
      日本分子生物学会第32回年会
    • Place of Presentation
      神奈川県・横浜市
    • Year and Date
      2009-12-09
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] ES/EG細胞試験管内分化系を用いたゲノム刷り込み型遺伝子発現の解析2009

    • Author(s)
      三瀬名丹、近藤昌代、阿部訓也
    • Organizer
      日本遺伝学会第81回大会
    • Place of Presentation
      長野県・松本市
    • Year and Date
      2009-09-17
    • Related Report
      2011 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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