Molecular mechanisms of the redox regulation of translation and the response of photosynthesis to environmental changes

• Project/Area Number: 21570033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: NISHIYAMA Yoshitaka 埼玉大学, 大学院・理工学研究科, 准教授 (30281588)
• Co-Investigator(Renkei-kenkyūsha): HISABORI Toru 東京工業大学, 資源化学研究所, 教授 (40181094)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 色素体機能 / 光合成 / 翻訳系 / EF-G / レドックス制御 / 光化学系II / 酸化ストレス / タンパク質合成 / ラン藻 / チオレドキシン

Research Abstract

Elongation factor G, a key protein in translation elongation, was inactivated via the formation of an intramolecular disulfide bond between two specific cysteine residues under oxidative conditions in the cyanobacterium Synechocystissp. PCC 6803. Reduction of the disulfide bond by thioredoxin resulted in the reactivation of EF-G, suggesting that translation might be regulated by the reducing power derived from the photosynthetic electron transport. Replacement of the target cysteine residue of EF-G by serine in Synechocystiscells protected photosystem II from photoinhibition.

Research Products

• [Journal Article] A change in the sensitivity of elongation factor G to oxidation protects photosystem II from photoinhibition in Synechocystis sp (2012)
  • Author(s): Ejima, K., Kawaharada, T., Inoue, S., Kojima, K., Nishiyama, Y.
  • Journal Title: FEBS Lett. Volume: 586(6) Pages: 778-783
  • Peer Reviewed
• [Journal Article] The mechanism of photoinhibition in vivo : re-evaluation of the roles of catalase, α-tocopherol, non-photochemical quenching, and electron transport (2012)
  • Author(s): Murata N., Allakhverdiev S.I., Nishiyama Y.
  • Journal Title: Biochim. Biophys. Acta Volume: (in press) Issue: 8 Pages: 1127-1133
  • DOI: 10.1016/j.bbabio.2012.02.020
  • Peer Reviewed
• [Journal Article] A change in the sensitivity of elongation factor G to oxidation protects photosystem II from photoinhibition in Synechocystis sp.PCC 6803 (2012)
  • Author(s): Ejima, K., Kawaharada, T., Inoue, S., Kojima, K., Nishiyama, Y.
  • Journal Title: FEBS Letters Volume: 586 Issue: 6 Pages: 778-783
  • DOI: 10.1016/j.febslet.2012.01.042
  • Peer Reviewed
• [Journal Article] Protein synthesis is the primary target of reactive oxygen species in the photoinhibition of photosystem II (2011)
  • Author(s): Nishiyama Y., Allakhverdiev S.I., Murata N.
  • Journal Title: Physiol. Plant Volume: 142 Pages: 35-46
  • Peer Reviewed
• [Journal Article] Protection by α-tocopherol of the repair of photosystem II during photoinhibition in Synechocystis sp (2011)
  • Author(s): Inoue S., Ejima K., Iwai E., Hayashi H., Appel J., Tyystjarvi E., Murata N., Nishiyama Y.
  • Journal Title: Biochim. Biophys. Acta Volume: 1807 Pages: 236-241
  • Peer Reviewed
• [Journal Article] Protection by α-tocopherol of the repair of photosystem II during photoinhibition in Synechocystis sp.PCC 6803 (2011)
  • Author(s): Inoue, S., Ejima, K., Iwai, E., Hayashi, H., Appel, J., Tyystjarvi, E., Murata, N., Nishiyama, Y.
  • Journal Title: Biochimica et Biophysica Acta-Bioenergetics Volume: 1807 Issue: 2 Pages: 236-241
  • DOI: 10.1016/j.bbabio.2010.11.003
  • Peer Reviewed
• [Journal Article] Protein synthesis is the primary target of reactive oxygen species in the photoinhibition of photosystem II (2011)
  • Author(s): Nishiyama, Y., Allakhverdiev, S.I, Murata, N.
  • Journal Title: Physiologia Plantarum Volume: 142 Issue: 1 Pages: 35-46
  • DOI: 10.1111/j.1399-3054.2011.01457.x
  • Peer Reviewed
• [Journal Article] Protection by α-tocopherol of the repair of photosystem II during photoinhibition in Synechocystis sp.PCC 6803 (2011)
  • Author(s): Inoue, S., Ejima, K., Iwai, E., Hayashi, H., Appel, J., Tyystjarvi, E., Murata, N., Nishiyama, Y.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1807 Pages: 236-241
  • Peer Reviewed
• [Journal Article] Protein synthesis is the primary target of reactive oxygen species in the ohotoinhibition of nhotosystem II (2011)
  • Author(s): Nishiyama, Y., Allakhverdiev, S.I., Murata, N.
  • Journal Title: Physiologia Plantarum Volume: 142 Pages: 35-46
  • Peer Reviewed
• [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp. (2010)
  • Author(s): Nanjo Y., Mizusawa N., Wada H., Slabas A.R., Hayashi H., Nishiyama Y.
  • Journal Title: Biochim. Biophys. Acta Volume: 1797 Pages: 1483-1490
  • Peer Reviewed
• [Journal Article] Differential proteomic analysis using iTRAQ reveals changes in thylakoids associated with Photosystem II acquired thermotolerance in Synechocystis sp (2010)
  • Author(s): Rowland J. G., Simon W. J., Nishiyama Y., Slabas A. R.
  • Journal Title: Proteomics Volume: 10 Pages: 1917-1929
  • Peer Reviewed
• [Journal Article] The PedR transcription factor interacts with thioredoxin to link photosynthesis with gene expression (2010)
  • Author(s): Horiuchi M., Nakamura K., Kojima K., Nishiyama Y., Hatakeyama W., Hisabori T., Hihara Y.
  • Journal Title: Biochem. J. Volume: 431(1) Pages: 135-140
• [Journal Article] Differential proteomic analysis using iTRAQ reveals changes in thylakoids associated with Photosystem II acquired thermotolerance in Synechocystis sp.PCC 6803 (2010)
  • Author(s): Rowland, J.G., Simon, W.J., Nishiyama, Y., Slabas, A.R.
  • Journal Title: Proteomics Volume: 10 Pages: 1917-1929
  • Peer Reviewed
• [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp. PCC 6803 to high temperature (2010)
  • Author(s): Nanjo, Y., Mizusawa, N., Wada, H., Slabas, A.R., Hayashi, H., Nishiyama, Y.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1797 Pages: 1483-1490
  • Peer Reviewed
• [Journal Article] The PedR transcription factor interacts with thioredoxin to link photosynthesis with gene expression (2010)
  • Author(s): Horiuchi, M., Nakamura, K., Kojima, K., Nishiyama, Y., Hatakeyama, W., Hisabori, T., Hihara, Y.
  • Journal Title: The Biochem.Journal Volume: 431(1) Pages: 135-140
  • Peer Reviewed
• [Journal Article] Differential proteomic analysis using iTRAQ reveals changes in thylakoids associated with Photosystem II acquired thermotolerance in Synechocystis sp.PCC 6803 (2010)
  • Author(s): Rowland, J.G., Simon, W.J., Nishiyama, Y., Slabas, A.R.
  • Journal Title: Proteomics (印刷中)
  • Peer Reviewed
• [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp.PCC 6803 to high temperature (2010)
  • Author(s): Nanjo, Y., Mizusawa, N., Wada, H., Slabas, A.R., Hayashi, H., Nishiyama, Y.
  • Journal Title: Biochimica et Biophysica Acta (印刷中)
  • Peer Reviewed
• [Journal Article] Regulation of translation by the redox state of elongation factor G in the cyanobacterium Synechocystis (2009)
  • Author(s): Kojima, K., Motohashi, K., Morota, T., Oshita, M., Hisabori, T., Hayashi, H., Nishiyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 284(28) Pages: 18685-18691
  • Peer Reviewed
• [Journal Article] Physiological impact of thioredoxin-and glutaredoxin-mediated redox regulation in cyanobacteria (2009)
  • Author(s): Nishiyama Y., Hisabori T.
  • Journal Title: In Advances in Botanical Research (Elsevier) Volume: 52 Pages: 187-205
  • Peer Reviewed
• [Journal Article] Regulation of translation by the redox state of elongation factor G in the cyanobacterium Synechocystis sp.PCC 6803 (2009)
  • Author(s): Kojima, K., Motohashi, K., Morota, T., Oshita, M., Hisabori, T., Hayashi, H., Nishiyama, Y.
  • Journal Title: Journal of Biological Chemistry 284(28) Pages: 18685-18691
  • Peer Reviewed
• [Journal Article] Physiological impact of thioredoxin- and glutaredoxin-mediated redox regulation in cyanobacteria (2009)
  • Author(s): Nishiyama, Y., Hisabori, T.
  • Journal Title: Advances in Botanical Research 52 Pages: 187-205
  • Peer Reviewed
• [Presentation] タンパク質合成系の改変による光化学系IIの強光耐性の向上 (2012)
  • Author(s): 江島加余子, 西山佳孝
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] 光化学系IIの光阻害に対する抗酸化物質の役割 (2012)
  • Author(s): 井上修平, Jens Appel, 高市真一, 村田紀夫, 西山佳孝
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] 穏やかな高温ストレス下における光化学系IIの光阻害の緩和 (2012)
  • Author(s): 上野護, 西山佳孝
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] 翻訳因子EF-Gによる翻訳制御機構 (2011)
  • Author(s): 永野孝典, 小島幸治, 林秀則, 金森崇, 宮城智子, 上田卓也, 西山佳孝
  • Organizer: 種を超えて存在するレドックス制御
  • Place of Presentation: かずさDNA研究所
  • Year and Date: 2011-12-02
• [Presentation] 翻訳因子EF-Gによる翻訳制御機構-種を超えて存在するレドックス制御- (2011)
  • Author(s): 永野孝典、小島幸治、林秀則、金森崇、宮城智子、上田卓也、西山佳孝
  • Organizer: ラン藻の分子生物学2011
  • Place of Presentation: かずさDNA研究所
  • Year and Date: 2011-12-02
• [Presentation] 大腸菌翻訳因子EF-Gの酸化傷害と翻訳制御 (2011)
  • Author(s): 永野孝典, 小島幸治, 林秀則, 金森崇, 宮城智子, 上田卓也, 西山佳孝
  • Organizer: 第6回無細胞生命科学研究会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス
  • Year and Date: 2011-11-17
• [Presentation] 大腸菌翻訳因子EF-Gのレドックス翻訳制御機構 (2011)
  • Author(s): 永野孝典, 小島幸治, 林秀則, 金森崇, 宮城智子, 上田卓也, 西山佳孝
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] シアノバクテリア翻訳系のレドックス制御と環境応答 (2011)
  • Author(s): 西山佳孝
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] 光化学系IIの光阻害に対するα-トコフェロールの役割 (2011)
  • Author(s): 井上修平, Jens Appel, 林秀則, 村田紀夫, 西山佳孝
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2011-09-17
• [Presentation] 光合成とタンパク質合成系の環境応答 (2011)
  • Author(s): 西山佳孝
  • Organizer: 埼玉大学環境科学研究センターシンポジウム
  • Place of Presentation: 埼玉大学
  • Year and Date: 2011-07-21
• [Presentation] Role of reactive oxygen species in the regulation of photosynthesis and protein synthesis under photoinhibition (2011)
  • Author(s): Y. Nishiyama, N. Murata
  • Organizer: 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
  • Place of Presentation: ブBudapest, Hungary
  • Year and Date: 2011-07-07
• [Presentation] Role of reactive oxygen species in the regulation of photosynthesis and protein synthesis under photoinhibitlon (2011)
  • Author(s): 西山佳孝、村田紀夫
  • Organizer: 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
  • Place of Presentation: ブダペスト(ハンガリー)
  • Year and Date: 2011-07-07
• [Presentation] 光化学系IIの光阻害におけるα-トコフェロールの保護作用 (2011)
  • Author(s): 井上修平, 江島加余子, Jens Appel, 林秀則, 村田紀夫, 西山佳孝
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-21
• [Presentation] 光合成の強光ストレス応答における翻訳因子EF-Gの役割 (2011)
  • Author(s): 江島加余子, 西山佳孝
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Presentation] シアノバクテリア翻訳因子EF-Gの光合成依存的なレドックス制御 (2011)
  • Author(s): 諸田拓哉, 小島幸治, 日原由香子, 本橋健, 畠山和佳子, 久堀徹, 西山佳孝
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Presentation] Interactive regulation of protein synthesis and photosynthesis under strong light (2011)
  • Author(s): Y. Nishiyama
  • Organizer: Japanese-Finnish Seminar 2011
  • Place of Presentation: Okayama
  • Year and Date: 2011-03-04
• [Presentation] Interactive regulation of protein synthesis and photosynthesis under strong light (2011)
  • Author(s): Nishivama. Y.
  • Organizer: JAPANESE-FINNISH Seminar 2011 Future prospects of photosynthetic organisms : from environment
  • Place of Presentation: 岡山ロイヤルホテル
  • Year and Date: 2011-03-04
• [Presentation] Roles of elongation factor G in the response of protein synthesis and photosynthesi to strong light in Synechocystis sp (2011)
  • Author(s): K. Ejima, Y. Nishiyama
  • Organizer: Japanese-Finnish Seminar
  • Place of Presentation: Okayama
  • Year and Date: 2011-03-02
• [Presentation] Roles of elongation factor G in the response of protein synthesis and photosynthesis to strong light in Synechocystis sp.PCC 6803 (2011)
  • Author(s): Kayoko Ejima, Nishivama, Y.
  • Organizer: JAPANESE-FINNISH Seminar 2011 Future prospects of photosynthetic organisms : from nenomes to environment
  • Place of Presentation: 岡山ロイヤルホテル
  • Year and Date: 2011-03-02
• [Presentation] α-トコフェロールによる光化学系II光阻害の防御機構 (2010)
  • Author(s): 井上修平, J. Appel, 村田紀夫, 西山佳孝
  • Organizer: 第1回日本光合成学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-06-04
• [Presentation] 光化学系IIの光阻害に対する低温・高温ストレスの影響 (2010)
  • Author(s): 上野護, 西山佳孝
  • Organizer: 第1回日本光合成学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-06-04
• [Presentation] α-トコフェロールによる光化学系II光阻害の防御機構 (2010)
  • Author(s): 井上修平、Jens Appel、村田紀夫、西山佳孝
  • Organizer: 第1回日本光合成学会大会
  • Place of Presentation: 東京大学(駒場)
  • Year and Date: 2010-06-04
• [Presentation] 光化学系IIの光阻害に対する低温・高温ストレスの影響 (2010)
  • Author(s): 上野護、西山佳孝
  • Organizer: 第1回日本光合成学会大会
  • Place of Presentation: 東京大学(駒場)
  • Year and Date: 2010-06-04
• [Presentation] シアノバクテリアの強光応答における翻訳因子EF-Gの役割 (2010)
  • Author(s): 江島加余子, 西山佳孝
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] シアノバクテリアの強光応答における翻訳因子EF-Gの役割 (2010)
  • Author(s): 江島加余子、西山佳孝
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] 強光条件下におけるシアノバクテリアの翻訳因子EF-Gのレドックス制御 (2010)
  • Author(s): 諸田拓哉, 小島幸治, 日原由香子, 本橋健, 畠山和佳子, 久堀徹, 西山佳孝
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-19
• [Presentation] 強光条件下におけるシアノバクテリアの翻訳因子EF-Gのレドックス制御 (2010)
  • Author(s): 諸田拓哉、小島幸治、日原由香子、本橋健、畠山和佳子、久堀徹、西山佳孝
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-19
• [Presentation] Responses of photosystem II to high temperature and strong light in cyanobacteria (2010)
  • Author(s): Y. Nishiyama
  • Organizer: Department Seminar
  • Place of Presentation: University of Turku, Turku, Finland
  • Year and Date: 2010-03-17
• [Presentation] Responses of Photosystem II to high temperature and strong light in cyanobacteria (2010)
  • Author(s): Nishiyama, Y.
  • Organizer: Department Seminar, University of Turku
  • Place of Presentation: フィンランド・トゥルク
  • Year and Date: 2010-03-17
• [Presentation] 光化学系IIの高温適応における脂肪酸合成の役割 (2009)
  • Author(s): 西山佳孝
  • Organizer: かずさDNA研究所研究会「ラン藻の分子生物学2009」
  • Place of Presentation: かずさDNA研究所
  • Year and Date: 2009-12-05
• [Presentation] 光合成の強光応答における翻訳因子EF-Gの役割 (2009)
  • Author(s): 江島加余子, 西山佳孝
  • Organizer: かずさDNA研究所研究会「ラン藻の分子生物学2009」
  • Place of Presentation: かずさDNA研究所
  • Year and Date: 2009-12-05
• [Presentation] 光化学系IIの光阻害に対するα-トコフェロールの保護作用 (2009)
  • Author(s): 井上修平, 林秀則, 村田紀夫, 西山佳孝
  • Organizer: かずさDNA研究所研究会「ラン藻の分子生物学2009」
  • Place of Presentation: かずさDNA研究所
  • Year and Date: 2009-12-05
• [Remarks]
  • URL: http://www.molbiol.saitama-u.ac.jp/nishiyama/kankyo/Top.html