Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions
Project/Area Number |
21570034
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Saitama University |
Principal Investigator |
NISHIDA Ikuo 埼玉大学, 大学院・理工学研究科, 教授 (40189288)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | ホスファチジルコリン / ホスファチジルエタノールアミン / 低温応答 / リン脂質合成 / 根の伸長 / 表皮細胞の内膜構造 / CDP-コリン合成 / ホスファチジルセリン / リン脂質合成のしくみ / 根の表皮細胞 / 内膜構造 |
Research Abstract |
WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana2011
Author(s)
Yamaoka, Y., Yu, Y., Mizoi, J., Fujiki, Y., Saito, K., Nishijima, M., Lee, Y., Nishida, I.
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Journal Title
The Plant Journal
Volume: In press
Related Report
Peer Reviewed
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[Journal Article] Isozyme-specifc modes of activation of CTP : phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature2009
Author(s)
Inatsugi, R., Kawai, H., Yamaoka, Y., Yu, Y., Sekiguchi, A., Nakamura, M., Nishida, I.
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Journal Title
Plant Cell Physiol 50
Pages: 1727-1735
Related Report
Peer Reviewed
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[Presentation] Physiological Significance of CTP : Phosphorylcholine Cytidylytransferase Genes in Arabidopsis Roots under Stress Conditions2010
Author(s)
Nishida, I., Mori, Y., Tsukano, Y., Yu, Y., Kawai, H., Yamaoka, Y, Lee, Y., Fujiki, Y.
Organizer
19th International Symposium on Plant Lipids
Place of Presentation
Cairns Australia
Year and Date
2010-07-11
Related Report
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[Presentation] The effects of pect1-4mutation on the lipid composition and the respiration activity of isolated mitochondria2010
Author(s)
Yu, Y., Kawai, H., Kawamoto, M., Mizoi, J., Fujiki, Y., Nishida, I.
Organizer
第51回日本植物生理学会年会
Place of Presentation
熊本
Related Report
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[Presentation] CTP : phosphorylcholine cytidylyltransferase genes are important for endomembrane development in Arabidopsis thaliana root cells2009
Author(s)
Nishida, I., Tsukano, J., Mori, Y., Yamaoka, Y., Yu, Y., Kawai, H., Fujiki, Y.
Organizer
The Third Asian Symposium on Platn Lipids
Place of Presentation
Yokohama Media and Communications Center
Year and Date
2009-11-28
Related Report
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