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Role of Mg-chelatase on intracellular signal transduction

Research Project

Project/Area Number 21570035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

MASUDA Tatsuru  東京大学, 大学院・総合文化研究科, 准教授 (00242305)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsクロロフィル / Mg-キラターゼ / 葉緑体 / Genome-uncoupled / 遺伝子 / Genome uncoupled / genomu uncoupled / プラスチド / プラスチドシグナル / ポルフィリン / genome uncoupled / 組換えタンパク質
Research Abstract

Mg-chelatase, which is composed by CHLI, CHLD, and CHLH subunits, catalyzes the the first committed step of chlorophyll biosynthetic pathway. In plants, it is known that the expression of nuclear-encoded photosynthesis related genes is coupled with chloroplast functionality. In Arabidopsis, mutants termed gun (genome uncoupled) were identified in which intracellular signaling was disrupted. Screening of gun mutants revealed many mutations were originated in mis-sense mutation of CHLH, suggesting Mg-chelatase functions not only for chlorophyll biosynthesis but also for the intracellular chloroplast to nucleus signal transduction (retrograde signaling).In this study, we have constructed reconstitution system of Mg-chelatase activity and revealed that gun phenotype is mainly related to the repression of Mg-chelatase activity. However, it is not clear why such repression caused the accumulation of CHLH protein in vivo. Analysis of functionality and conformation of Mg-chelatase complex will give novel insight into the retrograde signaling pathway in plant cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (45 results)

All 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (23 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis by impaired cytokinin metabolism2012

    • Author(s)
      Hedtke, B., Alawady, A., Albacete, A., Kobayashi, K., Melzer, M., Roitsch,T., Masuda, T., and Grimm, B
    • Journal Title

      Plant Mol. Biol

      Volume: 78 Pages: 77-93

    • Related Report
      2011 Final Research Report
  • [Journal Article] Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis2012

    • Author(s)
      Kobayashi, K., Baba, S., Obayashi, T., Keranen, M., Aro, E.M., Fukaki, H., Ohta, H., and Masuda, T
    • Journal Title

      Plant Cell

      Volume: 24 Pages: 1081-1095

    • Related Report
      2011 Final Research Report
  • [Journal Article] Role of the G-box element for regulation of chlorophyll biosynthesis in Arabidopsis root2012

    • Journal Title

      Plant Signal. Behavior

      Volume: 7 Pages: 922-926

    • Related Report
      2011 Final Research Report
  • [Journal Article] Evaluation of unbound free heme by differential extraction method2012

    • Author(s)
      Espinas, A. N., Kobayashi, K., Takahashi, S., Mochizuki, N., and Masuda, T
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Pages: 1344-1354

    • Related Report
      2011 Final Research Report
  • [Journal Article] Disrupting the bimolecular binding of the heme binding protein 5 (AtHBP5) with heme oxygenase I (HYI) leads to oxidative stress in Arabidopsis2012

    • Author(s)
      Lee H.-Y., Masuda, T., and Buckhout, T. J
    • Journal Title

      J Exp Bot

      Volume: 63 Pages: 5967-5978

    • Related Report
      2011 Final Research Report
  • [Journal Article] Tetrapyrrole metabolism in Arabidopsis thaliana2011

    • Author(s)
      Tanaka, R., Kobayashi, K., and Masuda, T
    • Journal Title

      The Arabidopsis Book

      Volume: 9

    • Related Report
      2011 Final Research Report
  • [Journal Article] Tetrapyrrole metabolism in Arabidopsis thaliana2011

    • Author(s)
      Tanaka, Kobayashi, Masuda
    • Journal Title

      The Arabidopsis Book

      Volume: 9 Pages: e0145-e0145

    • DOI

      10.1199/tab.0145

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cell biology of tetrapyrroles: a life and death struggle2010

    • Author(s)
      Mochizuki, M., Tanaka, R., Grimm, B., Masuda, T., Moulin, M., Smith, A. G., Tanaka, A., and Terry, M. J.
    • Journal Title

      Trends in Plant Sci

      Volume: 15 Pages: 488-498

    • Related Report
      2011 Final Research Report
  • [Journal Article] Genomic structure of an economically important cyanobacterium, Arthrospira (Spirulina) platensis NIES-392010

    • Author(s)
      Fujisawa N, Masuda T, et al
    • Journal Title

      DNA research

      Volume: 17 Pages: 85-103

    • NAID

      130006992893

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cell biology of tetrapyrroles : a life and death struggle2010

    • Author(s)
      Mochizuki N, Masuda T, et al
    • Journal Title

      Trends in Plant Science

      Volume: 15 Pages: 488-498

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 産業的に重要なシアノバクテリアArthrospira platensis NIES-39(通称スピルリナ)のゲノムの多様な特徴:基礎から応用まで、個々の遺伝子から比較ゲノムまで2010

    • Author(s)
      成川礼、増田建, 他
    • Journal Title

      光合成研究

      Volume: 59 Pages: 150-160

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis.2009

    • Author(s)
      Masuda, S., Ikeda, R., 増田建, Hashimoto, H., Tsuchiya, T., Kojima, H., Nomata, J., Fujita, Y., Mimuro, M., Ohta, H., Takamiya, K.
    • Journal Title

      The Plant Journal 58

      Pages: 952-960

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-throughput heme assay by detection of chemiluminescence of reconstituted horseradish peroxidase.2009

    • Author(s)
      Takahashi, S., 増田建
    • Journal Title

      Combinatorial Chemistry and High Throughput Screening 12

      Pages: 532-535

    • NAID

      130006990883

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Type-B monogalactosyldiacylglycerol synthases are involved in phosphate starvation-induced lipid remodeling, and are crucial for low-phosphate adaptation.2009

    • Author(s)
      小林康一, Awai, K., Nakamura, M., Nagatani, A., 増田建, Ohta, H.
    • Journal Title

      The Plant Journal 57

      Pages: 322-331

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Interaction of Arabidopsis heme binding protein 5 (HBP5) with heme oxygenase 1 (HY1) in plastids2012

    • Author(s)
      Lee, H.J., Masuda, T., Mochizuki, N.,Buckhout, T
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Interaction of Arabidopsis heme binding protein 5 (HBP5) with heme oxygenase 1 (HYl) in plastids2012

    • Author(s)
      Lee, Masuda, Mochizuki, Buckhout
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大学(京都)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Tetrapyrrole in plants-Stories about chlorophyll and heme2011

    • Author(s)
      Masuda
    • Organizer
      International Seminar at CNRS Strasbourg
    • Place of Presentation
      Strasbourg, France(招待講演)
    • Year and Date
      2011-08-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of chloroplast biogenesis in the root of GLK (Golden-2 like) overexpressed Arabidopsis2011

    • Author(s)
      Kobayashi, K., Masuda, T
    • Organizer
      2012 International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] Tetrapyrrole in plants -Stories about chlorophyll and heme2011

    • Author(s)
      Masuda, T
    • Organizer
      International Seminar at CNRS Strasbourg
    • Place of Presentation
      Strasbourg, France
    • Related Report
      2011 Final Research Report
  • [Presentation] Mg-キラターゼのサブユニットであるCHLHのシロイヌナズナgun変異体における蓄積2011

    • Author(s)
      池邨、堀、増田
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Heme regulation is strictly controlled in Arabidopsis ferrochelatase deficient mutants,fc1 and fc22011

    • Author(s)
      Espinas, N., Mochizuki, N., Masuda, T
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物からのヘム抽出法の検討-植物細胞におけるフリーヘムプールの評価-2011

    • Author(s)
      増田、小林、Espinas,高橋、望月
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] エンドウ葉緑体画分とシロイヌナズナ組換えタンパク質によるMg-キラターゼ活性の再構成と調節機構の解析2011

    • Author(s)
      池邨、増田
    • Organizer
      第52回植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of chloroplast biogenesis in the root of GLK (Golden-2like) overexpressed Arabidopsis2011

    • Author(s)
      Kobayashi, Masuda
    • Organizer
      2012 International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物からのヘム抽出法の検討-植物細胞におけるフリーヘムプールの評価-2011

    • Author(s)
      増田、小林、Espinas, 高橋、望月
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)
    • Related Report
      2011 Annual Research Report
  • [Presentation] エンドウ葉緑体画分とシロイヌナズナ組換えタンパク質によるMg-キラターゼ活性の再構成と調節機構の解析2011

    • Author(s)
      池邨友理子、増田建
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of Arabidopsis thaliana Ferrochelatase Genes, FC1 and FC2, and its Role in Chlorophyll and Heme Biosynthesis Pathway2011

    • Author(s)
      Espinas NA, Mochizuki N, Masuda T
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mg-キラターゼのCHLHサブユニットとポルフィリンの複合体は光分解を受ける2010

    • Author(s)
      堀恵悟、池邨友理子、望月伸悦、増田建
    • Organizer
      第51回植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 有用シアノバクテリアArthrospira(Spirulina)platensis NIES-39のゲノム解析2010

    • Author(s)
      成川礼、藤澤貴智、岡本忍、得平茂樹、吉村英尚、鈴木岩根、増田建、持丸真理、高市真一、粟井光一郎、関根光雄
    • Organizer
      第51回植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] GARP型転写因子Golden-2 like(GLK)の発現制御によるシロイヌナズナの根での可塑的な葉緑体分化2010

    • Author(s)
      小林康一、深城英弘、野口航、増田建
    • Organizer
      第51回植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] MgキラターゼのCHLHサブユニットとポルフィリン複合体は光分解を受ける2010

    • Author(s)
      堀、池邨、望月、増田
    • Organizer
      第51回植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2011 Final Research Report
  • [Presentation] シロイヌナズナの根におけるクロロフィル合成制御機構2009

    • Author(s)
      小林康一、馬場信輔、Eva-Mari Aro、深城英弘、太田啓之、増田建
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナにおけるMg-キラターゼのCHLHサブユニットの生化学的解析2009

    • Author(s)
      堀恵悟、望月伸悦、久堀徹、増田建
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナのエチレン及びヘム関連変異体を用いた傷害リグニン蓄積機構の解析2009

    • Author(s)
      大森丈子、増田建、佐藤康
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物組織からのプロトヘム抽出とその高感度検出2009

    • Author(s)
      増田建、高橋重一、小林康一
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Coordinated regulation of chlorophyll biosynthesis by light and phytohormones in Arabidopsis2009

    • Author(s)
      増田建, 深城英弘, 小林康一
    • Organizer
      Tetrapyrrole Photoreceptors of Photosynthetic Organisms, ICTPPO 2009
    • Place of Presentation
      Asilomar Conference Center, USA
    • Year and Date
      2009-07-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Extraction and quantification of protoheme from plant tissues2009

    • Author(s)
      高橋重一, 小林康一, 増田建
    • Organizer
      Tetrapyrrole Photoreceptors of Photosynthetic Organisms, ICTPPO 2009
    • Place of Presentation
      Asilomar Conference Center, USA
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Book] The Handbook of Porphyrin Science2011

    • Author(s)
      Kobayashi, K., and Masuda, T.
    • Publisher
      Tetrapyrrole biosynthesis in plant systems
    • Related Report
      2011 Final Research Report
  • [Book] 図説生物学Biology Illustrated2010

    • Author(s)
      「図説生物学」編集委員会
    • Total Pages
      234
    • Publisher
      東京大学出版会
    • Related Report
      2010 Annual Research Report
  • [Book] 生物学辞典2010

    • Author(s)
      石川統, 他編集
    • Total Pages
      1615
    • Publisher
      東京化学同人
    • Related Report
      2010 Annual Research Report
  • [Book] 二次代謝物の生合成と機能inベーシックマスター 植物生理学(塩井祐三・井上弘・近藤矩朗 共編)2009

    • Author(s)
      増田建
    • Total Pages
      19
    • Publisher
      オーム社
    • Related Report
      2009 Annual Research Report
  • [Book] ヘムの定量in光合成研究法(「光合成研究法」編集委員会編)2009

    • Author(s)
      高橋重一, 増田建
    • Total Pages
      11
    • Publisher
      北海道大学 低温科学研究所
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab/Masuda_Laboratory/Welcome.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab/Masuda_Laboratory/Welcome.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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