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Signal Transduction Pathway Regulating Dorsal-Ventral Axis Formation in Guard Cells

Research Project

Project/Area Number 21570046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionShimane University

Principal Investigator

NAKAGAWA Tsuyoshi  島根大学, 総合科学研究支援センター, 教授 (30202211)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords植物ホルモン / 成長整理 / 全能性 / 植物 / シロイヌナズナ / 気孔 / 孔辺細胞 / 形態形成 / 受容体型キナーゼ / 低分子量分泌タンパク質 / 信号伝達 / 微小管 / 形熊形成
Research Abstract

Stomata are small pores exist on the surface of leaf in plants. Stomata work for intake of carbon dioxide necessary for photosynthesis from atmosphere. Stomata are important organs for plant growth and maintenance of environment. They are also interesting research targets in plant development. Stomata are formed by oppositely positioned two guard cells. The development of guard cell starts from protodermal cell. During development, guard cell becomes longer and bent outside to make pore. At this stage guard cell obtains dorsal-ventral polarity. According to this polarity, cell wall and cytoskeleton begin to distribute to make specific cell shape. In this study, we found a factor contributing polarity formation and analyzed its function. The polarity of guard cell was firstly established by asymmetric localization of a receptor like kinase on the cell membrane of guard cell. We also found that the specific localization pattern was caused by the kinase domain of the receptor like kinase.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 7 results) Presentation (32 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Expression analysis of Arabidopsis thaliana small secreted protein genes2012

    • Author(s)
      Nakamura, S., Suzuki, T., Kawamukai, M. and Nakagawa, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Pages: 436-446

    • NAID

      10030750247

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Two Sec13 homologs, AtSec13A and AtSec13B, redundantly contribute to the formation of COPII transport vesicle in Arabidopsis thaliana2011

    • Author(s)
      Hino, T., Tanaka, Y., Kawamukai, M., Nishimura, K., Mano, S. and Nakagawa, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Pages: 1848-1852

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Development of a series of Gateway binary vectors possessing a tunicamycin resistance gene as a marker for the transformation of Arabidopsis thaliana2011

    • Author(s)
      Tanaka, Y., Nakamura, S., Kawamukai, M., Koizumi, N., Nakagawa, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Pages: 804-807

    • NAID

      10028272038

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Novel vector systems to accelerate functionall analysis of transcription factors using chimeric repressor gene-silencing technology (CRES-T)2011

    • Author(s)
      Oshima, Y., et al.
    • Journal Title

      Plant Biotechnology

      Volume: 28 Pages: 201-210

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Live-cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana2011

    • Author(s)
      Hamamura, Y., et al.
    • Journal Title

      Current Biology

      Volume: 21 Pages: 497-502

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gateway binary vectors with the bialaphos resistance gene bar, as a selection marker for plant transformation2010

    • Author(s)
      Nakamura, S., Mano, S., Tanaka, Y., Ohnishi, M., Nakamori, C., Araki, M., Niwa, T., Nishimura, M., Kaminaka, H., Nakagawa, T., Sato, Y. and Ishiguro, S.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Pages: 1315-1319

    • NAID

      10027556769

    • Related Report
      2011 Final Research Report
  • [Journal Article] Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation2010

    • Author(s)
      Nakamura S., et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Pages: 1315-1319

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-Coenxyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains2010

    • Author(s)
      Ishiguro, S., et al.
    • Journal Title

      Plant and Cell Physiology

      Volume: 51 Pages: 896-911

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Gateway binary vectors, R4L1pGWBs, for promoter analysis in higher plants2009

    • Author(s)
      Nakamura, S., Nakao, A., Kawamukai, M., Kimura, T., Ishiguro, S. and Nakagawa, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 73 Pages: 2556-2559

    • NAID

      10027548374

    • Related Report
      2011 Final Research Report
  • [Journal Article] Gateway vectors for plant transformation2009

    • Author(s)
      Nakagawa, T., Ishiguro, S. and Kimura, T.
    • Journal Title

      Plant Biotechnology

      Volume: 26 Pages: 275-284

    • NAID

      10024853760

    • Related Report
      2011 Final Research Report
  • [Journal Article] Development of Gateway Binary Vectors, R4L1pGWBs, for promoter analysis in highter plants.2009

    • Author(s)
      Nakamura S., et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73

      Pages: 2556-2559

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli : functional evolution of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.2009

    • Author(s)
      Okada, S., et al.
    • Journal Title

      Plant Cell Physiology 50

      Pages: 1049-1061

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gateway vectors for plant transformation (Invited review)2009

    • Author(s)
      Nakagawa T., et al.
    • Journal Title

      Plant Biotechnology 26

      Pages: 275-284

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物形質転換用リサイクルベクターシステムの開発2012

    • Author(s)
      芝原健太, ら
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナキチン受容体の細胞内分子挙動の解析2012

    • Author(s)
      安川大喜, ら
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] BiFC法によるシロイヌナズナクラスリンサブユニット間の相互作用の解析2012

    • Author(s)
      石川翔太, ら
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナの花器官発達におけるCOPII小胞輸送の役割2012

    • Author(s)
      田中優史, ら
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Recycling vector system for multi-gene construct. Stable expression of multi-gene in plant cells-2011

    • Author(s)
      Nakao, A., Shibahara, K., Kato, K., Tachiki, K., Kimura T., Nakagawa, T.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 複数遺伝子導入が可能な植物形質転換用リサイクルベクターシステムの開発と安定発現条件の検討2011

    • Author(s)
      中尾彰秀, ら
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 分裂酵母でのCoQ10生産性の検討2011

    • Author(s)
      細野耕司, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都(非開催)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナクラスリン被覆の構造解析2011

    • Author(s)
      石川翔太, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都(非開催)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナのクラスリン軽鎖と重鎖の相互作用の解析2011

    • Author(s)
      西村浩二
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      第52回年会講演要旨集
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナの発達におけるCOPII構成因子ATSEC23の機能2011

    • Author(s)
      田中優史, ら
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      第52回年会講演要旨集
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物形質転換用リサイクルベクターシステムの開発2011

    • Author(s)
      中尾彰秀、加藤晃、立木賢輔、木村哲哉、中川強
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2011 Final Research Report
  • [Presentation] ビアラフォス、ツニカマイシン選択が可能な植物形質転換用Gatewayバイナリベクターシリーズの開発2011

    • Author(s)
      田中優史、中村真也、真野昌二、大西真人、川向誠、佐藤豊、石黒澄衞、小泉望、中川強
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2011 Final Research Report
  • [Presentation] ホルムアルデヒド(HCHO)を固定し同化する代謝酵素の合成遺伝子を導入したイネの作成と解析2011

    • Author(s)
      鈴木詩織, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      2011年度大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビアラフォス、ツニカマイシン選択が可能な植物形質転換用Gatewayバイナリベクターシリーズの開発2011

    • Author(s)
      田中優史, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      2011年度大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物形質転換用リサイクルベクターシステムの開発2011

    • Author(s)
      中尾彰秀, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      2011年度大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分裂酵母の有性生殖過程を亢進させるsam変異の原因遺伝子の同定2011

    • Author(s)
      大石和義, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      2011年度大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] CoQ整合性に必須なPHB:ポリプレニルニリン酸転移酵素の特性2011

    • Author(s)
      林和弘, ら
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      2011年度大会講演要旨集
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物の気孔形成に働く受容体型キナーゼ信号伝達系の解析2010

    • Author(s)
      中村真也、川向誠、中川強
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-12
    • Related Report
      2011 Final Research Report
  • [Presentation] ビアラフォス耐性遺伝子(bar)をもった植物形質転換用Gatewayバイナリーベクターの開発2010

    • Author(s)
      田中優史、中村真也、真野昌二、大西真人、佐藤豊、石黒澄衞、中川強
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の気孔形成に働く受容体型キナーゼ信号伝達系の解析2010

    • Author(s)
      中村真也, ら
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビアラフォス耐性遺伝子(bar)をたった植物形質転換用Gatewayバイナリーベクターの開発2010

    • Author(s)
      田中優史, ら
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 白色腐朽菌リグニン分解酵素遺伝子の植物への導入とその解析2010

    • Author(s)
      領木智哉
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物用2遺伝子クローニングシステムの開発と利用2010

    • Author(s)
      中尾彰秀, 中村真也, 川向誠, 木村哲哉, 中川強
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 分裂酵母のコエンザイムQ生合成不能株における硫黄代謝制御2010

    • Author(s)
      古田奈々, ら
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高等真核生物のコエンザイムQ生合成遺伝子の同定2010

    • Author(s)
      林和弘, ら
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物用2遺伝子クローニングシステムの開発と利用2010

    • Author(s)
      中尾彰秀, ら
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナにおけるアスコルビン酸応答遺伝子の探索2010

    • Author(s)
      高用順, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナCOPll輸送小胞の被覆構成因子ATSEC23, ATSEC24の解析2010

    • Author(s)
      田中優史, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物の気孔形成に働く受容体型キナーゼ信号伝達系の解析2009

    • Author(s)
      中村真也, 川向誠, 中川強
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の気孔形成に働く受容体型キナーゼ信号伝達系の解析2009

    • Author(s)
      中村真也, ら
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナのCOPll被覆構成因子ATSEC23, ATSEC24の解析2009

    • Author(s)
      田中優史, ら
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 分裂酵母の有性生殖を亢進させるsam2変異株の原因遺伝子のスクリーニング2009

    • Author(s)
      大石和義, ら
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Book] Gateway vectors for plant genetic engineering : Overview of plant vectors, application for Bimolecular Fluorescence Complementation (BiFC) and multigene construction. In Genetic Engineering, Genetic Engineering-Basics, New Applications and Responsibilities2012

    • Author(s)
      Tanaka, Y., et al
    • Total Pages
      24
    • Publisher
      Intech Open Access Publisher
    • Related Report
      2011 Annual Research Report
  • [Book] Gateway vectors for plant genetic engineering : Overview of plant vectors, application for Bimolecular Fluorescence Complementation(BiFC) and multigene construction2011

    • Author(s)
      Tanaka, Y., Kimura, T., Hikino, K., Goto, S., Nishimura, M., Mano, S. and Nakagawa, T.
    • Publisher
      Intech Open Access Publisher
    • Related Report
      2011 Final Research Report
  • [Book] 植物ベクター開発への応用「Gatewayを用いた遺伝子導入マニュアル」2009

    • Author(s)
      中村真也, 中川強
    • Publisher
      Springer Japan
    • Related Report
      2011 Final Research Report
  • [Book] 植物ベクターへの応用「Gatewayを用いた遺伝子導入マニュアル」2009

    • Author(s)
      中村真也、中川強
    • Total Pages
      2
    • Publisher
      シュプリンガージャパン
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://shimane-u.org/nakagawa/index.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://shimane-u.org/nakagawa/index.htm

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://shimane-u.org/nakagawa/index.htm

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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