Study on stringent response in plant chloroplasts

• Project/Area Number: 21570047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Ehime University
• Principal Investigator: TOZAWA Yuzuru 愛媛大学, 無細胞生命科学工学研究センター, 教授 (90363267)
• Research Collaborator: YUKAWA Yasushi 名古屋市立大学, 大学院・システム自然科学研究科, 教授 (70381902) ; NOMURA Yuhta 愛媛大学, 大学院・理工学研究科, 博士後期課程2年生
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 環境応答 / 葉緑体 / ppGpp / 翻訳 / 緊縮制御 / カルシウム / 翻訳制御 / 転写制御 / 植物葉緑体 / 緊縮制御因子 / 植物葉縁体

Research Abstract

Chloroplasts are derived from symbiosis of photosynthetic bacteria, and have ppGpp-mediated signal transduction system. In order to clarify the functional roles of ppGpp-signal in chloroplasts, we have investigated functions of ppGpp in the protein synthesis. Here we have established in vitro translation system based on extracts of isolated pea chloroplasts, and demonstrated that ppGpp directly regulates protein synthesis activity of chloroplasts in vitro.

Research Products

• [Journal Article] ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro (2012)
  • Author(s): Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y
  • Journal Title: Plant Mol Biol Volume: 78 Pages: 185-196
  • Peer Reviewed
• [Journal Article] Expression of a small(p) ppGpp synthetase, YwaC, in the(p) ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome (2012)
  • Author(s): Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Tozawa Y, Morimoto, T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa H, Kuroiwa T, Ohashi Y, Kawamura F
  • Journal Title: MicrobiologyOpen
  • Peer Reviewed
• [Journal Article] Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome (2012)
  • Author(s): Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Tozawa Y, Morimoto, T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa H, Kuroiwa T, Ohashi Y, Kawamura F
  • Journal Title: MicrobiologyOpen Volume: (印刷中) Issue: 2 Pages: 115-134
  • DOI: 10.1002/mbo3.16
  • Peer Reviewed
• [Journal Article] ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro (2012)
  • Author(s): Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y
  • Journal Title: Plant Molecular Biology Volume: 78 Issue: 1-2 Pages: 185-196
  • DOI: 10.1007/s11103-011-9858-x
  • Peer Reviewed
• [Journal Article] Signaling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants (2011)
  • Author(s): Tozawa Y, and Nomura Y
  • Journal Title: Plant Biology Volume: 13 Pages: 609-709
  • Peer Reviewed
• [Journal Article] Signaling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants (2011)
  • Author(s): Yuzuru Tozawa, Yuhta Nomura
  • Journal Title: Plant Biology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] 高等植物におけるppGpp合成系とその生理学的機能解析をめざして (2010)
  • Author(s): 戸澤譲, 笠井光治
  • Journal Title: 植物の生長調節 Volume: 42巻 Pages: 119-124
• [Journal Article] 高等植物におけるppGpp合成系とその生理学的機能の解明をめざして (2010)
  • Author(s): 戸澤譲, 笠井光治
  • Journal Title: 植物の生長調節 Volume: 42 Pages: 119-124
  • NAID: 110008006480
  • Peer Reviewed
• [Journal Article] Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient of(p) ppGpp synthetase genes, relA, yjbM and ywaC (2009)
  • Author(s): Natori Y, Tagami K, Murakami K, Yoshida S, Tanigawa O, Moh Y, Masuda K, Wada T, Suzuki S, Nananamiya H, Tozawa Y, and Kawamura F
  • Journal Title: Journal of Bacteriology Volume: 191 Pages: 4555-4561
  • Peer Reviewed
• [Journal Article] Transcriptional activity of individual rrn operons in Bacillus subtilis (2009)
  • Author(s): Natori Y, Tagami K, Murakami K, Yoshida S, Tanigawa O, Moh Y, Masuda K, Wada T, Suzuki S, Nanamiya H, Tozawa Y, Kawamura F.
  • Journal Title: Journal of Bacteriology 191 Pages: 4555-4561
  • Peer Reviewed
• [Presentation] 植物オルガネラ膜輸送体蛋白質の完全インビトロ合成・再構成系による機能解析 (2012)
  • Author(s): 野村勇太、高林泰斗、戸澤譲
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] エンドウマメ葉緑体インビトロ翻訳系の構築と翻訳阻害剤の影響評価 (2012)
  • Author(s): 野村勇太, 高林泰斗, 戸澤譲
  • Organizer: 日本農芸化学会2012年度大会
• [Presentation] Establishment of pea chloroplast "organelle-free" protein synthesis system for assessment of translation inhibitors (2011)
  • Author(s): Yuhta Nomura, Taito Takabayashi, Hiroshi Kuroda, Yasushi Yukawa, Kwanchanok Sattasuk, Mitsuru Akita, Akira Nozawa, Yuzuru Tozawa
  • Organizer: The 9th Matsuyama International Symposium on Cell-Free Sciences
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2011-09-22
• [Presentation] In vitro翻訳系による葉緑体タンパク質合成制御系の解析 (2011)
  • Author(s): 野村勇太、戸澤譲
  • Organizer: 平成23年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2011-09-16
• [Presentation] 植物葉緑体の翻訳系制御におけるppGppの機能的役割の解析 (2011)
  • Author(s): 野村勇太、高林泰斗、戸澤譲
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] Establishment of pea chloroplast" organelle-free" protein synthesis system for assessment of translation inhibitors (2011)
  • Author(s): Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y
  • Organizer: The 9th Matsuyama International Symposium on Cell-Free Sciences
• [Presentation] in vitro翻訳系による葉緑体タンパク質合成制御系の解析 (2011)
  • Author(s): 野村勇太, 戸澤譲
  • Organizer: 平成23年度日本農芸化学会西日本支部・中四国支部合同大会
• [Presentation] 植物葉緑体におけるppGppの機能的役割の解析 (2011)
  • Author(s): 野村勇太, 高林泰斗, 戸澤譲
  • Organizer: 日本農芸化学会2011年度大会
• [Presentation] 植物およびバクテリアのppGpp定量法 (2010)
  • Author(s): 野村勇太, 及川彰, 戸澤譲
  • Organizer: 日本農芸化学会2010年度大会
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/biomolecular/biomolecular_eng_rsc1.html