Analysis of tubular elongation in the network of endoplasmic reticulum

• Project/Area Number: 21570049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: University of Hyogo
• Principal Investigator: YOKOTA Etsuo 兵庫県立大学, 大学院・生命理学研究科, 助教 (80212299)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 小胞体 / レティキュロン / RHD3 / プロテインキナーゼ / タンパク質リン酸化 / ダイナソー / GTP / リン酸化 / シロイヌナズナ / キナーゼ / ミオシン / タバコ培養細胞BY-2 / GTPアーゼ / アトラスチン / 小胞体チューブ / ダイナミン

Research Abstract

In the cultured cell of Arabidopsis, MM2d, it was shown that reticulon-like proteins(AtRTN) and RHD3, a homologue of atlastin GTPase, are involved in the tubular formation of endoplasmic reticulum(ER), as is case of yeast and animal cells. The RHD3 was able to bind to GTP, and form the oligomer under the conditions in which ER tubules were organized. Furthermore, ER tubule formation was suggested to be regulated by the phosphorylation.

Research Products

• [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured cells, BY-2 (2011)
  • Author(s): E. Yokota, H. Ueda, K. Hashimoto, H. Orii, T. Shimada, I. Hara-Nishimura, T. Shimmen
  • Journal Title: Plant Physiol Volume: 156 Issue: 1 Pages: 129-143
  • DOI: 10.1104/pp.111.175018
  • Peer Reviewed
• [Journal Article] The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea (2011)
  • Author(s): Hashimoto, K., Yokota, E., Shimmen, T., and Yoshida, M.
  • Journal Title: Biotechnol. Lett. Volume: 33 Issue: 4 Pages: 769-775
  • DOI: 10.1007/s10529-010-0497-0
  • Peer Reviewed
• [Journal Article] Effect of myosin ATPase inhibitor 2,3-butanedione 2-monoxime on protein secretion of basidiomycete Coprinopsis cinerea. (2011)
  • Author(s): K.Hashimoto
  • Journal Title: Biotechnology Letters Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured cells, BY-2. (2011)
  • Author(s): E.Yokota
  • Journal Title: Plant Physiol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells (2010)
  • Author(s): Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawa, S., Dolja, V. V., and Hara-Nishimura, I.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Issue: 15 Pages: 6894-6899
  • DOI: 10.1073/pnas.0911482107
  • NAID: 120002511346
  • Peer Reviewed
• [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells. (2010)
  • Author(s): H.Ueda
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 6894-6899
  • Peer Reviewed
• [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells (2010)
  • Author(s): Ueda, Haruko
  • Journal Title: Proceeding of the National Academy of Sciences, USA (印刷中)
  • Peer Reviewed
• [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco (2009)
  • Author(s): Hamada, T., Igarashi, H., Taguchi, R., Fujiwara, M., Fukao, Y., Shimmen, T., Yokota, E., and Sonobe. S.
  • Journal Title: Plant Cell Physiol Volume: 50 Issue: 4 Pages: 801-811
  • DOI: 10.1093/pcp/pcp024
  • NAID: 10027339590
  • Peer Reviewed
• [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells (2009)
  • Author(s): E. Yokota, S. Ueda, K. Tamura, H. Orii, S. Uchi, S. Sonobe, I. Hara-Nishimura, T. Shimmen
  • Journal Title: J. Exp. Bot Volume: 60 Pages: 197-212
  • Peer Reviewed
• [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis (2009)
  • Author(s): I. Karahara, J. Suda, H. Tahara, E. Yokota, T. Shimmen, K. Misaki, S. Yonemura, T. Murata, L. A. Staehelin, Y. Mineyuki
  • Journal Title: Plant J Volume: 57 Pages: 819-831
  • Peer Reviewed
• [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells (2009)
  • Author(s): Yokota, Etsuo
  • Journal Title: Journal of Experimental Botany 60 Pages: 197-212
  • Peer Reviewed
• [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco (2009)
  • Author(s): Hamada, Takahiro
  • Journal Title: Plant and Cell Physiology 50 Pages: 801-811
  • Peer Reviewed
• [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis (2009)
  • Author(s): Karahara, Ichirou
  • Journal Title: The Plant Journal 57 Pages: 819-831
  • Peer Reviewed
• [Presentation] 植物細胞における小胞体ダイナミクス (2012)
  • Author(s): 上田晴子
  • Organizer: 第14回植物オルガネラワークショップ
  • Place of Presentation: 京都大益川ホール(京都府)
  • Year and Date: 2012-03-15
• [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化 (2011)
  • Author(s): 上田晴子
  • Organizer: 日本生化学会第84回大会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-09-21
• [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化 (2011)
  • Author(s): 上田晴子
  • Organizer: 日本生化学会第84回大会
  • Place of Presentation: 京都国際会館(京都府)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] 接合菌類ヒゲカビの原形質流動を担うミオシンの同定 (2011)
  • Author(s): 横田悦雄
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学駒場キャンパス(東京都)
  • Year and Date: 2011-09-18
• [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin organizations (2011)
  • Author(s): H. Ueda
  • Organizer: International Botanical Congress
  • Place of Presentation: メルボルンン(オーストラリア)
  • Year and Date: 2011-07-26
• [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin organizations (2011)
  • Author(s): 上田晴子
  • Organizer: International Botanical Congress 2011
  • Place of Presentation: メルボルン(オーストラリア)(招待講演)
  • Year and Date: 2011-07-26
• [Presentation] 植物細胞における小胞体流動～定量解析と生理学的意義～ (2010)
  • Author(s): 上田晴子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県)
  • Year and Date: 2010-09-09
• [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割 (2010)
  • Author(s): 上田晴子
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-21
• [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割 (2010)
  • Author(s): 上田晴子
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] 小胞体チューブ状構造形成におけるアクチン系細胞骨格の役割 (2009)
  • Author(s): 横田悦雄
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2009-09-19
• [Presentation] 小胞体チューブ状構造形成におけるアクチン系細胞骨格の役割 (2009)
  • Author(s): 横田悦雄
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-09-19
• [Book] Plant myosins. In The Plant Cytoskeletons. (2011)
  • Author(s): E.Yokota
  • Total Pages: 24
  • Publisher: Springer
• [Book] The Plant Cytoskeletons (2010)
  • Author(s): E. Yokota, T. Shimmen
  • Publisher: Springer
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biomecha/index-jhtml