Biogenesis of plant peroxisomes mediated by PEX16

• Project/Area Number: 21570053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: HAYASHI Makoto 基礎生物学研究所, 高次細胞機構研究部門, 准教授 (50212155)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ペルオキシソーム / グリオキシソーム / 脂質分解 / 光呼吸 / オルガネラ / 緑葉ペルオキシソーム / 脂肪酸β酸化 / タンパク質輸送

Research Abstract

Plant peroxisome is an organelle involved in important metabolisms such as lipid breakdown and photorespiration. I have identified many peroxins that regulate biogenesis of plant peroxisome. Here I tried to identify proteins cooperatively work together with these peroxins. As the results, I found PEX12, PEX13 and RabE1c as binding partners with PEX7. I also found several proteins bind with PEX16, and am keep analyzing function of these proteins. In addition, method to screen suppressor mutants from GFP-PEX16 overexpression line was established

Research Products

• [Journal Article] Proteomic analysis reveals that the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEROXIN 7 (2013)
  • Author(s): Cui, S., Fukao, Y., Mano, S., Yamada, K., Hayashi, M. and Nishimura, M
  • Journal Title: J. Biol Volume: 288 Pages: 6014-6023
  • Peer Reviewed
• [Journal Article] Proteomic analysis reveals the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEX7 (Peroxin 7) (2013)
  • Author(s): Soungkui Cui, Yoichiro Fukao, Shoji Mano, Kenji Yamada, Makoto Hayashi and Mikio Nishimura
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 8 Pages: 6014-6023
  • DOI: 10.1074/jbc.m112.438143
  • Peer Reviewed
• [Journal Article] Loss of XRN4 Function Can Trigger Cosuppression in a Sequence-dependent Manner. Plant Cell Physiol (2012)
  • Author(s): Hayashi, M., Nanba, C., Saito M., Kondo, M., Takeda, A.,Watanabe Y. and Nishimura, M.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 1310-1321
  • Peer Reviewed
• [Journal Article] A peroxisomal ABC transporter promotes seed germination by inducing pectin degradation under the control of ABI5 (2010)
  • Author(s): Kanai, M., Nishimura, M. and Hayashi, M
  • Journal Title: Plant J Volume: 62 Pages: 936-947
  • Peer Reviewed
• [Journal Article] Molecular components required for the targeting of PEX7 to peroxisomes in Arabidopsis thaliana (2009)
  • Author(s): Singh, T., Hayashi, M., Mano, S., Arai, Y. and Nishimura, M
  • Journal Title: Plant J Volume: 60 Pages: 488-498
  • Peer Reviewed
• [Journal Article] Peroxisomes are required for in vivo nitric oxide (NO) accumulation in the cytosol following salinity stress of Arabidopsis plants (2009)
  • Author(s): Corpas, F.J., Hayashi, M., Mano, S., Nishimura, M. and Barroso, J.B
  • Journal Title: Plant Physiol Volume: 151 Pages: 2083-2094
  • Peer Reviewed
• [Journal Article] Suppression of peroxsiome biogenesis factor 10 reduces cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana (2009)
  • Author(s): Kamigaki, A., Kondo, M., Mano, S., Hayashi, M. and Nishimura, M
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 2034-2046
  • Peer Reviewed
• [Journal Article] 植物ペルオキシソームの機能多様性とその制御 (2009)
  • Author(s): 林 誠、西村幹夫
  • Journal Title: Plant Morphology Volume: 21 Pages: 41-46
  • NAID: 130002152914
• [Journal Article] SQUAMOSA promoter binding protein-like7 is a central regulator for copper homeostasis in Arabidopsis. (2009)
  • Author(s): Yamasaki, H., ら
  • Journal Title: Plant Cell Volume: 21 Pages: 347-361
  • Peer Reviewed
• [Journal Article] High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases. (2009)
  • Author(s): Hida, N., ら
  • Journal Title: PLoS ONE Volume: 4
  • Peer Reviewed
• [Journal Article] Molecular components required for the targeting of PEX7 to peroxisomes in Arabidopsis thaliana. (2009)
  • Author(s): Singh, T., ら
  • Journal Title: Plant J. Volume: 60 Pages: 488-498
  • Peer Reviewed
• [Journal Article] Peroxisomes are required for in vivo nitric oxide (NO) accumulation in the cytosol following salinity stress of Arabidopsis plants. (2009)
  • Author(s): Corpas, F.J., ら
  • Journal Title: Plant physiol. Volume: 151 Pages: 2083-2094
  • Peer Reviewed
• [Journal Article] Suppression of peroxsiome biogenesis factor 10 reduces cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana. (2009)
  • Author(s): Kamigaki, A., ら
  • Journal Title: Plant Cell Physiol. Volume: 50 Pages: 2034-2046
  • Peer Reviewed
• [Journal Article] Frontiers of research on organelle differentiation. (2009)
  • Author(s): Hayashi, M., Nishimura, M.
  • Journal Title: Plant Cell Physiol. Volume: 50 Pages: 1995-1999
  • NAID: 10027344578
• [Presentation] ペルオキシソーム局在型benzoyltransferaseはベンゼノイド産生を触媒する (2013)
  • Author(s): 中井篤、林 誠、深尾陽一郎、吉瀬(新井)祐子、西村幹夫
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] Identification and functional characterization of novel factors regulating peroxisomal protein receptor PEX7 (2013)
  • Author(s): Soungkui Cui, Yoichiro Fukao, Shoji Mano, Kenji Yamada, Makoto Hayashi and Mikio Nishimura
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] ペルオキシソーム局在異常変異体peup4の解析 (2013)
  • Author(s): 及川和聡、柴田美智太郎、近藤真紀、真野昌二、林 誠、吉本光希、大隅良典、西村幹夫
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] 種子特異的な乾燥耐性を担う遺伝子DPS1の同定とその機能解析 (2013)
  • Author(s): 金井雅武、林 誠、西村幹夫
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山）
• [Presentation] ペルオキシソーム局在型benzoyltransferaseはベンゼノイド生産を触媒する (2013)
  • Author(s): 中井篤、林 誠、深尾陽一郎、古瀬（新井）祐子、西村幹夫
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山）
• [Presentation] Identification and functional characterization of novel factors regulating peroxisomal protein receptor PEX7 (2013)
  • Author(s): Soungkui Cui, Yoichiro Fukao, Shoji Mano, Kenji Yamada, Makoto Hayashi and Mikio Nishimura
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山）
• [Presentation] ペルオキシソーム局在異常変異体peup4の解析 (2013)
  • Author(s): 及川和聡、柴田美智太郎、近藤真紀、真野昌二、 林 誠、吉本光希、大隅良典、西村幹夫
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山）
• [Presentation] 定量プロテオミクスを用いたダイズ子葉のペルオキシソーム機能転換の解析 (2012)
  • Author(s): 中井篤、林 誠、深尾陽一郎、吉瀬(新井)祐子、西村幹夫
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] Identification of novel peroxisomal biogenesis factors binding to PEX7 (2012)
  • Author(s): Soungkui Cui, Yoichiro Fukao, Makoto Hayashi and Mikio Nishimura
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] HS3は葉緑体ゲノムの転写制御により種子の貯蔵脂質合成に関与する (2011)
  • Author(s): 金井雅武, ら
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2011-03-20
• [Presentation] ペルオキシソーム局在異常変異体peup2とpeup4の解析 (2011)
  • Author(s): 及川和聡, ら
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2011-03-20
• [Presentation] 定量プロテオミクスを用いたダイズ子葉のペルオキシソームタンパク質の網羅的解析 (2011)
  • Author(s): 中井篤, ら
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2011-03-20
• [Presentation] HS3は葉緑体ゲノムの転写制御により種子の貯蔵脂質合成に関与する (2011)
  • Author(s): 金井雅武,林 誠,西村幹夫
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] 定量プロテオミクスを用いたダイズ子葉のペルオキシソームタンパク質の網羅的解析 (2011)
  • Author(s): 中井 篤、林 誠、深尾陽一郎、吉瀬(新井)祐子、西村幹夫
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] ペルオキシソーム局在異常変異体peup2とpeup4の解析 (2011)
  • Author(s): 及川和聡、柴田美智太郎、近藤真紀、吉本光希、真野昌二、林 誠、大隅良典、西村幹夫
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] Proteome as a tool for studying plant peroxisomes (2010)
  • Author(s): Hayashi, M., ら
  • Organizer: 2nd international symposium on "Frontier in Agriculture Proteome Research"
  • Place of Presentation: 作物研究所(茨城)
  • Year and Date: 2010-11-18
• [Presentation] ペルオキシソーム膜タンパク質の網羅的解析による新規輸送タンパク質の同定 (2010)
  • Author(s): 吉瀬(新井)祐子, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] Proteomic identification of novel peroxisome biogenesis factors (2010)
  • Author(s): Songkui C., ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] 光に依存したペルオキシソームとミトコンドリア・葉緑体との接着機構は光合成により制御される (2010)
  • Author(s): 及川和聡, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] ダイズ子葉のペルオキシソーム機能変換におけるペルオキシソームタンパク質の網羅的解析 (2010)
  • Author(s): 中井篤, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] PED3はABI5を介して種子発芽を制御する (2010)
  • Author(s): 金井雅武, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] Proteomic identification of novel peroxisome biogenesis factors (2010)
  • Author(s): Songkui C., Fukao, Y., Hayashi, M. and Nishimura, M
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] 光に依存したペルオキシソームとミトコンドリア、葉緑体との接着機構は光合成により制御される (2010)
  • Author(s): 及川和聡、松永茂、真野昌二、林 誠、近藤真紀、香川貴俊、坂本亘、東正一、渡辺正勝、西村幹夫
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] ダイズ子葉のペルオキシソーム機能変換におけるペルオキシソームタンパク質の網羅的解析 (2010)
  • Author(s): 中井篤、林 誠、深尾陽一郎、吉瀬(新井)祐子、西村幹夫
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] PED3はABI5を介して種子発芽を制御する (2010)
  • Author(s): 金井雅武,西村幹夫,林 誠
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] Peroxisomal targeting of PEX7, a receptor for PTS2-containing proteins, to peroxisomes (2009)
  • Author(s): Hayashi, M., ら
  • Organizer: International conference on Arabidopsis research
  • Place of Presentation: Edinburgh International Conference Centre, Edingurgh (UK)
  • Year and Date: 2009-06-30
• [Presentation] PED3 is required for process of breaking dormancy (2009)
  • Author(s): Kanai, M., ら
  • Organizer: International conference on Arabidopsis research
  • Place of Presentation: Edinburgh International Conference Centre, Edingurgh (UK)
  • Year and Date: 2009-06-30
• [Presentation] Peroxisomal targeting of PEX7, a receptor for PTS2-containing proteins, to peroxisomes, International conference on Arabidopsis research (2009)
  • Author(s): Hayashi, M., Singh, T., Mano, S., Arai, Y., Goto, S. and Nishimura, M
  • Organizer: Edingurgh
  • Place of Presentation: UK
• [Presentation] PED3 is required for process of breaking dormancy, International conference on Arabidopsis research (2009)
  • Author(s): Kanai, M., Nishimura, M. and Hayashi, M
  • Organizer: Edingurgh
  • Place of Presentation: UK
• [Remarks]
  • URL: http://www.nibb.ac.jp/~celmech/jp