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Gonadal plasticity of DMRT1 mutants in the medaka

Research Project

Project/Area Number 21570059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionUtsunomiya University

Principal Investigator

MATSUDA Masaru  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (20414013)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords形態形成 / 生殖腺性分化 / メダカ / 性決定 / DMRT1遺伝子 / 性差形成機構
Research Abstract

Dmrt1, which is found in many verte brates, exhibits testis-specific expression during the sexual differentiation period, suggesting a conserved function of Dmrt1 in the testicular development of vertebrate gonads. However, functional analyses have been reported only in mammals. The current study focused on the Dmrt1 function in the teleost medaka, Oryzias latipes, which has an XX-XY sex determina tion system. Although medaka sex is determined by the presence or absence of the Y chromosome-specific gene Dmy, we demonstrated that in one Dmrt1 mutant line, which was found by screening a gene-driven mutagenesis library, XY mutants developed into nor mal females and laid eggs. These results show that Dmrt1 is essential to maintain testis differentiation after Dmy-triggered male differentiation pathway.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (42 results)

All 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (20 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Expression of 3beta-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes)2012

    • Author(s)
      Nakamoto M, Fukasawa M, Tanaka S, Shimamori K, Suzuki A, Matsuda M, Kobayashi T, Nagahama Y, Shibata N
    • Journal Title

      Gen Comp Endocrinol

      Volume: 176 Pages: 222-230

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of 3beta-hydroxysteroid dehydrogenase (hsd3b), star and ad4 bp/sf-1 during gonadal development in medaka (Oryzias latipes)2012

    • Author(s)
      Nakamoto M, Fukasawa M, Tanaka S, Shimamori K, Suzuki A, Matsuda M, Kobayashi T, Nagahama Y, Shibata N
    • Journal Title

      General and Comparative Endocrinology

      Volume: 176 Pages: 222-230

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka2011

    • Author(s)
      Masuyama H, Yamada M, Kamei Y, Fujiwara-Ishikawa T, Todo T, Nagahama Y, Matsuda M
    • Journal Title

      Chromosome Res

      Volume: 20(1) Issue: 1 Pages: 163-76

    • DOI

      10.1007/s10577-011-9264-x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Sexually Dimorphic Expression of Genes at Early Gonadogenesis of Pejerrey Odontesthes bonariensis Using a Heterologous Microarray2011

    • Author(s)
      Fernandino JI, Popesku JT, Paul-Prasanth B, Xiong H, Hattori RS, Oura M, Strussmann CA, Somoza GM, Matsuda M, Nagahama Y, Trudeau VL
    • Journal Title

      Sex Dev

      Volume: 5 Pages: 89-101

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Sexually Dimorphic Expression of Genes at Early Gonadogenesis of Pejerrey Odontesthes bonariensis Using a Heterologous Microarray2011

    • Author(s)
      Fernandino JI, Popesku JT, Paul-Prasanth B, Xiong H, Hattori RS, Oura M, Strussmann CA, Somoza GM, Matsuda M, Nagahama Y, Trudeau VL
    • Journal Title

      Sexual Development

      Volume: 5 Issue: 2 Pages: 89-101

    • DOI

      10.1159/000324423

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Doublesex-and Mab-3-related transcription factor-1 repression of aromatase transcription, a possible mechanism favoring the male pathway in tilapia2010

    • Author(s)
      Wang DS, Zhou LY, Kobayashi T, Matsuda M, Shibata Y, Sakai F, Nagahama Y
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 1331-40

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka2010

    • Author(s)
      Shibata Y, Paul-Prasanth B, Suzuki A, Usami T, Nakamoto M, Matsuda M, Nagahama Y
    • Journal Title

      Gene Expr Patterns

      Volume: 10 Pages: 283-289

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] メダカの性決定2010

    • Author(s)
      松田勝
    • Journal Title

      水産育種

      Volume: 40 Pages: 3-9

    • NAID

      40017384654

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heritable artificial sex chromosomes in the medaka, Oryzias latipes2010

    • Author(s)
      Otake H, Masuyama H, Mashima Y, Shinomiya A, Myosho T, Nagahama Y, Matsuda M, Hamaguchi S, Sakaizumi M
    • Journal Title

      Heredity

      Volume: 105 Pages: 247-56

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of medaka cholesterol side chain cleavage cytochrome P450 during gonadal development2010

    • Author(s)
      Nakamoto M, Fukasawa M, Orii S, Shimamori K, Maeda T, Suzuki A, Matsuda M, Kobayashi T, Nagahama Y, Shibata N
    • Journal Title

      Develop. Growth Differ

      Volume: 52 Pages: 385-395

    • NAID

      10027564905

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Doublesex- and Mab-3-related transcription factor-1 repression of aromatase transcription, a possible mechanism favoring the male pathway in tilapia2010

    • Author(s)
      Wang, et al
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 1331-1340

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of gonadal soma derived factor (GSDF) is spatially and ternporally correlated with early testicular differentiation in medaka2010

    • Author(s)
      Shibata, et al
    • Journal Title

      Gene Expression Patterns

      Volume: 10 Pages: 282-289

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular phylogeny and genetic differentiation of Tanakia himantegus complex(Teleostei : Cyprinidae)in Taiwan and mainland China2009

    • Author(s)
      Chia-Hao Chang, et al
    • Journal Title

      Zoological Studies 48

      Pages: 839-850

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gonadal Sex Differentiation and Expression of Sox9a2, Dmrt1, and Foxl2 in Oryzias luzonensis2009

    • Author(s)
      Nakamoto, et al
    • Journal Title

      genesis 47

      Pages: 289-299

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heritable artificial sex chromosomes in the medaka, Oryzias latipes

    • Author(s)
      Otake, et al
    • Journal Title

      Heredity (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 野生メダカ由来XX雄系統の生殖腺分化2012

    • Author(s)
      松島美喜子,松田勝
    • Organizer
      日本動物学会第64回関東支部大会
    • Place of Presentation
      東邦大学習志野キャンパス
    • Year and Date
      2012-03-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Sex Determination by an Autosomal Gene Mutation2011

    • Author(s)
      Matsuda M
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2011-11-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Sex Determination by an Autosomal Gene Mutation2011

    • Author(s)
      Masaru Matsuda
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      Okazaki, Japan(招待講演)
    • Year and Date
      2011-11-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Establishment of FLAG-tagged Dmy Transgenic Medaka2011

    • Author(s)
      Imai T, Masuyama H, Matsuda M
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2011-11-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 融合DmyおよびDmrt1遺伝子導入メダカ系統の樹立2011

    • Author(s)
      齊野兼太郎,増山治男,松田勝
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] メダカGsdf遺伝子の転写制御領域の解析2011

    • Author(s)
      増山治男,北野健,松田勝
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] FLAGタグ融合Dmy遺伝子導入メダカの樹立2011

    • Author(s)
      今井拓人,増山治男,松田勝
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] A single basepair difference is sufficient to distinguish males from females in medaka fish2011

    • Author(s)
      Masuyama H, Yamada M, Kamei Y, Ishikawa T, Todo T, Nagahama Y, Matsuda M
    • Organizer
      17th Japanese Medaka and Zebrafish Meeting', Japan
    • Place of Presentation
      Mishima
    • Related Report
      2011 Final Research Report
  • [Presentation] DMRT1遺伝子突然変異メダカの解析2010

    • Author(s)
      増山治男,松田勝
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-23
    • Related Report
      2011 Final Research Report
  • [Presentation] メダカの性決定2010

    • Author(s)
      松田勝
    • Organizer
      水産育種研究会
    • Place of Presentation
      藤沢
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] DMRT1遺伝子突然変異メダカの解析2010

    • Author(s)
      増山治男,亀井保博,石川智子,藤堂剛,飯郷雅之,松田勝
    • Organizer
      第16回小型魚類研究会
    • Place of Presentation
      埼玉
    • Related Report
      2011 Final Research Report
  • [Presentation] DMRT1突然変異メダカの解析2009

    • Author(s)
      塚田亜矢,増山治男,山田雅人,松田勝
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] メダカ性決定遺伝子DMYは、いつ性分化の決め手となるのか?2009

    • Author(s)
      増山治男,松田勝
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Establishment of a transgenic line with the sex-determining gene, Dmy, in the teleost fish medaka, Oryzias latipes2009

    • Author(s)
      Masuyama H, Nagahama Y, Matsuda M
    • Organizer
      5th International Symposium on Vertebrate Sex Determination
    • Place of Presentation
      Kona. Hawaii, USA
    • Year and Date
      2009-04-23
    • Related Report
      2011 Final Research Report
  • [Presentation] DMRT1 IS REQUIRED FOR TESTICULAR DEVELOPMENT IN THE MEDAKA, ORYZIAS LATIPES2009

    • Author(s)
      Masaru Matsuda
    • Organizer
      5th International Symposium on Vertebrate Sex Determination
    • Place of Presentation
      Kona.Hawaii, USA
    • Year and Date
      2009-04-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] DMRT1遺伝子突然変異メダカはオスからメスへ性転換する2009

    • Author(s)
      増山治男,松田勝
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] A mutation in DMRT1 causes male to female sex reversal in medaka2009

    • Author(s)
      Masuyama H, Yamada M, Kamei Y, Ishikawa T, Todo T, Nagahama Y, Matsuda M
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Niigata
    • Related Report
      2011 Final Research Report
  • [Presentation] Conservation of regulatory mechanisms of DMY, the sex-determining gene in the medaka2009

    • Author(s)
      Otake H, Matsuda M, Sakaizumi M, Hamaguchi S
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Niigata
    • Related Report
      2011 Final Research Report
  • [Presentation] Dmrt1 is required for testicular development in the medaka, Oryzias latipes2009

    • Author(s)
      Matsuda M
    • Organizer
      5th International Symposium on Vertebrate Sex Determination
    • Place of Presentation
      Kona Hawaii, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Quick-change of sex chromosomes in the medaka2009

    • Author(s)
      Otake H, Matsuda M, Hamaguchi S, Sakaizumi M
    • Organizer
      5th International Symposium on Vertebrate Sex Determination
    • Place of Presentation
      Kona. Hawaii, USA
    • Related Report
      2011 Final Research Report
  • [Book] Medaka A model for Organogenesis, Human Disease, and Evolution2011

    • Author(s)
      Matsuda M
    • Total Pages
      267
    • Publisher
      The sex-determining gene in medaka. in K Naruse M Tanaka and H Takeda (eds.)
    • Related Report
      2011 Final Research Report
  • [Book] Chapter 17 The Sex Determining Gene in Medaka Medaka A model for Organogenesis, Human Disease, and Evolution2011

    • Author(s)
      Masaru Matsuda
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Book] Sex Determination. in BGM Jamieson (ed.) Reproductive Biology and Phylogeny of Fishes (Agnathans and Bony Fishes)2009

    • Author(s)
      Matsuda M, Sakaizumi M
    • Publisher
      Science Publishers
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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