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Osmoregulation in elephant fish : introduction of a new model animal for cartilaginous fish research

Research Project

Project/Area Number 21570061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionThe University of Tokyo

Principal Investigator

HYODO Susumu  東京大学, 大気海洋研究所, 准教授 (40222244)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords軟骨魚類 / 尿素 / ゾウギンザメ / 腎臓 / 初期発生 / ホルモン受容体 / 体液調節 / 腎機能 / 卵黄嚢上皮 / バソトシン受容体 / 尿素サイクル / 環境適応 / 脳下垂体神経葉ホルモン / 進化 / 輸送体分子 / 個体発生
Research Abstract

The aim of this study is to investigate adaptive, reproductive and developmental physiologies of the cartilaginous fish by using elephant fish as a model organism, for which the public genome database is available. We have proposed a urea reabsorption model in the renal nephron by mapping of various transporting proteins for urea, water and ions. In the present study, we also found contribution of the yolk-sac membrane to body fluid regulation in the early developmental stages, and a novel neurohypophysial hormone receptor that is likely to be involved in the maintenance of homeostasis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (53 results)

All 2012 2011 2010 2009 Other

All Journal Article (23 results) (of which Peer Reviewed: 20 results) Presentation (26 results) Remarks (4 results)

  • [Journal Article] The fifth neurohypophysial hormone receptor(V4R) is structurally related to the V2-type receptor but functionally similar to V1-and V3-type receptors2012

    • Author(s)
      Yamaguchi, Y., Kaiya, H., Konno, N., Iwata, E., Miyazato, M., Uchiyama, M., Bell, J. D., Toop, T., Donald, J. A., Brenner, S., Venkatesh, B., Hyodo, S.
    • Journal Title

      Gen. Comp. Endocrinol

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological and functional characterization of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure on the gill septum of Japanese banded houndshark, Triakis scyllium2012

    • Author(s)
      Souichirou Takabe, Keitaro Teranishi, Shin Takaki, Makoto Kusakabe, Shigehisa Hirose, Toyoji Kaneko, Susumu Hyodo
    • Journal Title

      Cell and Tissue Research

      Volume: 348 Issue: 1 Pages: 141-153

    • DOI

      10.1007/s00441-012-1344-5

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hepatic and extrahepatic distribution of ornithine urea cycle enzymes in holocephalan elephant fish2012

    • Author(s)
      Takagi, W., Kajimura, M., Bell, J. D., Toop, T., Donald, J. A., Hyodo, S.
    • Journal Title

      Comp. Biochem. Physiol. B

      Volume: 161 Issue: 4 Pages: 331-340

    • DOI

      10.1016/j.cbpb.2011.12.006

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the amount of thiotrophic symbionts in the deep-sea mussel Bathymodiolus septemdierum under different sulfide levels by using fluorescent in situ hybridization2012

    • Author(s)
      Fujinoki, M.
    • Journal Title

      Fisheries Science

      Volume: 78 Issue: 1 Pages: 139-146

    • DOI

      10.1007/s12562-011-0419-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei Possible physiological roles of the calcitonin family in osmoregulation2012

    • Author(s)
      Suzuki N, Sekiguchi T, Satake H, Kato K, Nishiyama Y, Takahashi H, et al
    • Journal Title

      Gene

      Volume: 499 Issue: 2 Pages: 326-331

    • DOI

      10.1016/j.gene.2012.03.042

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 軟骨魚類の生理学研究:体液調節を中心にライフサイクルを追う2010

    • Author(s)
      兵藤晋
    • Journal Title

      板鰓類研究会報

      Volume: 46 Pages: 1-7

    • NAID

      40022467509

    • Related Report
      2011 Final Research Report
  • [Journal Article] Vasotocin/V2-type receptor/aquaporin axis exists in African lungfish kidney but is functional only in terrestrial condition2010

    • Author(s)
      Konno, N., Hyodo, S., Yamaguchi, Y., Matsuda, K., Uchiyama, M.
    • Journal Title

      Endocrinology

      Volume: 151 Issue: 3 Pages: 1089-1096

    • DOI

      10.1210/en.2009-1070

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new method to calibrate attachment angles of data loggers in swimming sharks. Eurasip J. Advance in Signal2010

    • Author(s)
      Kawatsu, S., Sato, K., Watanabe, Y., Hyodo, S., Breves, J. P., Fox, B. K., Grau, E. G., Miyazaki, N.
    • Journal Title

      Processing

      Volume: Article ID 732586 Issue: 1 Pages: 732586-732586

    • DOI

      10.1155/2010/732586

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rhesus glycoprotein p2(Rhp2) is a novel member of the Rh family of ammonia transporters highly expressed in shark kidney2010

    • Author(s)
      Nakada, T., Westhoff, C. M., Yamaguchi, Y., Hyodo, S., Li, X., Muro, T., Kato, A., Nakamura, N., Hirose, S.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 4 Pages: 2653-2664

    • DOI

      10.1074/jbc.m109.052068

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rhesus glycoprotein p2 (Rhp2) is a novel member of the Rh family of ammonia trans porters highly expressed in shark kidne.2010

    • Author(s)
      Nakada, T.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 2653-2664

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new method to calibrate attachment angles of data loggers in swimmin sharks.2010

    • Author(s)
      Kawatsu, S.
    • Journal Title

      Eurasip Journal on Advances in Signal Processing

      Volume: 732586 Pages: 1-6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vasotocin/V2-type receptor/aquaporin axis exists in African lungfish kidney but is functional only in terrestrial condition.2010

    • Author(s)
      Konno, N.
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 1089-1096

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in prolactin mRNA levels during downstream migration of the amphidromous teleost, ayu Plecoglossus altivelis.2010

    • Author(s)
      Yada, T.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 167 Pages: 261-267

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 軟骨魚類の生理学研究:体液調節を中心にライフサイクルを追う2010

    • Author(s)
      兵藤晋
    • Journal Title

      板鯉類研究会報

      Volume: 46 Pages: 1-7

    • NAID

      40022467509

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Multiple urea transporter proteins in the kidney of holocephalan elephant fish. Comp2009

    • Author(s)
      Kakumura, K., Watanabe, S., Bell, J. D., Donald, J. A., Toop, T., Kaneko, T., Hyodo, S.
    • Journal Title

      Biochem. Physiol. B

      Volume: 154 Pages: 239-247

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular distribution of urea transporter in the collecting tubule of shark kidney is dependent on environmental salinity2009

    • Author(s)
      Yamaguchi, Y., Takaki, S., Hyodo, S.
    • Journal Title

      J. Exp. Zool

      Volume: 311A Issue: 9 Pages: 705-718

    • DOI

      10.1002/jez.558

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] African lungfish possess an arginine vasotocin receptor homologous to the tetrapod V2-type receptor2009

    • Author(s)
      Konno, N., Hyodo, S., Yamaguchi, Y., Kaiya, H., Miyazato, M., Matsuda, K., Uchiyama, M.
    • Journal Title

      J. Exp. Biol

      Volume: 212 Issue: 14 Pages: 2183-2193

    • DOI

      10.1242/jeb.029322

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extracellular calcium-sensing receptor distribution in osmoregulatory and endocrine tissues of the tilapia.2009

    • Author(s)
      Loretz, C.A.
    • Journal Title

      General and Comparative Endocrinology 161

      Pages: 216-228

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Centrally administrated adrenomedullin 5 activates oxytocin-secreting neurons in the hypothalamus and elevates plasma oxytocin levels in rats.2009

    • Author(s)
      Otsubo, H.
    • Journal Title

      Journal of Endocrinology 202

      Pages: 237-247

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] African lungfish(Protopterus annectens)possess an arginine vasotocin receptor homologous to the tetrapod V2-type receptor.2009

    • Author(s)
      Konno, N.
    • Journal Title

      Journal of Experimental Biology 212

      Pages: 2183-2193

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular distribution of urea transporter in the collecting tubule of shark kidney is dependent on environmental salinity.2009

    • Author(s)
      Yamaguchi, Y.
    • Journal Title

      Journal of Experimental Zoology 311A

      Pages: 705-718

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple urea transporter proteins in the kidney of holocephalan elephant fish(Callorhinchus milii).2009

    • Author(s)
      Kakumura, K.
    • Journal Title

      Comparative Biochemistry and Physiology B 154

      Pages: 239-247

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] オーストラリア・ハワイとの共同研究をとおした新たな軟骨魚類研究の推進2009

    • Author(s)
      兵藤晋
    • Journal Title

      月刊海洋 号外52

      Pages: 149-158

    • Related Report
      2009 Annual Research Report
  • [Presentation] 卵生軟骨魚類の発生過程における浸透圧調節2012

    • Author(s)
      高木亙
    • Organizer
      平成24年度日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2012-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ゾウギンザメ腎臓における尿素輸送体および尿素輸送関連分子の局在と機能2012

    • Author(s)
      角村佳吾
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2012-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ゾウギンザメ腎臓における尿素輸送体および尿素輸送関連分子の局在と機能2012

    • Author(s)
      角村佳吾
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 卵生軟骨魚類の発生過程における浸透圧調節2012

    • Author(s)
      高木亙
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脊椎動物における新規バソプレシン/オキシトシン受容体、V1cRファミリーの発見2012

    • Author(s)
      山口陽子
    • Organizer
      第22回バソプレシン研究会
    • Place of Presentation
      慶應義塾大学病院
    • Year and Date
      2012-01-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 脊椎動物における新規バソプレシン/バソトシン受容体V1cRファミリーの発見2012

    • Author(s)
      山口陽子
    • Organizer
      第22回バソプレシン研究会
    • Place of Presentation
      慶應義塾大学病院(東京都)(研究奨励賞受賞)
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脊椎動物における新規神経葉ホルモン受容体V1cRファミリーの発見-軟骨魚類の研究から-2011

    • Author(s)
      山口陽子
    • Organizer
      第36回日本比較内分泌学会、シンポジウム
    • Place of Presentation
      都道府県会館
    • Year and Date
      2011-11-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 脊椎動物における新規神経葉ホルモン受容体V1cRファミリーの発見-軟骨魚類の研究から2011

    • Author(s)
      山口陽子
    • Organizer
      第36回日本比較内分泌学会大会
    • Place of Presentation
      都道府県会館(東京都)(招待講演)
    • Year and Date
      2011-11-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ornithine urea cycle enzymes inholocephalan elephant fish2011

    • Author(s)
      Takagi, W.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Ornithine urea cycle enzymes in holocephalan elephant fish2011

    • Author(s)
      Takagi, W.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-06-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Morphological and functional characteristics of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure in the gill septum of Japanese banded hound shark2011

    • Author(s)
      Takabe, S.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-06-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Existence of multiple receptors for neurohypophysial hormones in cartilaginous fish2011

    • Author(s)
      Yamaguchi, Y.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Morphological and functional characteristics of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure in the gill septum of Japanese banded houndshark2011

    • Author(s)
      Takabe, S.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and mapping of urea-and ion-transporting molecules in the kidney of cartilaginous fish2011

    • Author(s)
      Hyodo, S.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-06-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification and mapping of urea- and ion-transporting molecules in the kidney of cartilaginous fish2011

    • Author(s)
      Hyodo, S.
    • Organizer
      International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場(愛知県)(招待講演)
    • Year and Date
      2011-06-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] サメ、エイ、ギンザメ:体液調節を中心にそのライフサイクルを追う2010

    • Author(s)
      兵藤晋
    • Organizer
      第11回日本比較三学会合同シンポジウム
    • Place of Presentation
      静岡グランシップ
    • Year and Date
      2010-11-20
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] アカエイのカルシトニンファミリー受容体のクローニングと発現解析2010

    • Author(s)
      鈴木信雄
    • Organizer
      第35回日本比較内分泌学会
    • Place of Presentation
      静岡グランシップ
    • Year and Date
      2010-11-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] トビハゼの水陸両生におけるバソトシンとイソトシンの役割2010

    • Author(s)
      西山雄大
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] ドチザメにおけるカルバミルリン酸合成酵素IIIの分布と環境塩分変化の影響2010

    • Author(s)
      山口陽子
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-23
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ドチザメの鯉隔膜上で発見した新規塩類細胞様集合体の機能2010

    • Author(s)
      高部宗一郎
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] ドチザメの脳における新規RFalnindeペプチドの同定2010

    • Author(s)
      大杉知裕
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] アカエイにおけるカルシトニンの生理的役割2010

    • Author(s)
      鈴木信雄
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] サメの鰓は塩分排出器官か2009

    • Author(s)
      兵藤晋
    • Organizer
      日本比較内分泌学会第34回大会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2009-10-23
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 肺魚で見つかった新規アクアポリン水チャネル遺伝子の構造と機能2009

    • Author(s)
      北村咲奈
    • Organizer
      日本比較内分泌学会第34回大会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 肺魚から見たアクアポリン遺伝子の分子進化2009

    • Author(s)
      今野紀文
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡グランシップ
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゾウギンザメは軟骨魚類研究のブレークスルーをもたらすか2009

    • Author(s)
      兵藤晋
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡グランシップ
    • Year and Date
      2009-09-18
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Remarks]

    • URL

      http://physiol.ori.u-tokyo.ac.jp/seiri/hyodo/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://physiol.ori.u-tokyo.ac.jp/seiri/hyoudo/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://physiol.ori.u-tokyo.ac.jp/seiri/hyoudo/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://physiol.ori.u-tokyo.ac.jp/seiri/hyoudo/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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