Studies of the functional roles of amphibian skin antimicrobial peptides as the first barrier against infection by environmental microorganisms.
| Project/Area Number |
21570068
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Sakae 早稲田大学, 教育・総合学術院, 名誉教授 (20063638)
KOBAYASHI Tetsuya 埼玉大学, 大学院・理工学研究科, 教授 (00195794)
HASUNUMA Itaru 東邦大学, 理学部, 講師 (40434261)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 比較内分泌 / 抗菌ペプチド / 免疫内分泌 / 生体防御 / 両生類 / 抗菌性ヒストン / ヒストン / 外膜プロテアーゼ / 膜透過ペプチド / ハーダー腺 / マスト細胞脱顆粒 / 抗腫瘍細胞活性 / 脱顆粒 / オキタゴガエル / ウシガエル / ニホンアカガエル / 変態 / ブラジキニン / 培養細胞 / シークエンスキャプチャー / ファブリキウス嚢 |
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| Research Abstract |
We have cloned and characterized cDNAs encoding multiple antimicrobial peptide precursors from total RNA samples prepared from the skin and some extradermal tissues such as brain and the Harderian gland of the Japanese Ranid frog species. The synthetic replicates of some of these peptides showed anti-tumor cell activities and/or mast cell-stimulating activities as well as antimicrobial activities. We investigated anti-Escherichia coli activities of the Lys-rich histone H2B and Arg-rich histones H3 and H4 as antimicrobial agents and showed that the mode of their antimicrobial actions depended on their basic amino acid residue contents.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article]2010
Author(s)
Iwamuro, S
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Journal Title
Methods Mol.Biol.615 : Peptidomics.An efficient protocol for DNA amplification of multiple amphibian skin antimicrobial peptide cDNAs(Springer)
Pages: 159-176
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[Presentation] ヒストン外伝-膜の彼方へ2010
Author(s)
岩室祥一
Organizer
第61回染色体学会公開シンポジウム
Place of Presentation
東邦大学習志野キャンパス(千葉県)(招待講演)
Year and Date
2010-11-18
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