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Integrative studies on mechanically mutable connective tissue

Research Project

Project/Area Number 21570075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionTokyo Institute of Technology

Principal Investigator

MOTOKAWA Tatsuo  東京工業大学, 生命理工学研究科, 教授 (80092352)

Project Period (FY) 2009-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords棘皮動物 / 結合組織 / 力学的性質 / ナマコ / 硬さ可変結合組織 / キャッチ結合組織 / 硬さ / ヒトデ / 酸素消費量 / 硬化因子 / ウニ
Research Abstract

Mechanically mutable connective tissue (CCT) takes 3 different mechanical states, soft (S), standard (M), and stiff (H). We have isolated from sea cucumbers "NSF", a 2.4 kDa-protein, that caused M to H transition and "softenin", a 20 kDa-protein, that caused M-to-S transition. From these and the images of electron microscopy, we proposed the following molecular mechanism for stiffness changes: the transition from S to M is caused by the aggregation of collagen molecules induced by tensilin, which is antagonized by softenin; the transition from M to H is caused by cross bridging between collagen fibrils by NSF.
The oxygen consumption of 3 states of CCT from 3 different animal classes were measured and compared to that of muscles. The amount to oxygen consumption was lowest in M and highest in S that was far lower than that of relaxed muscles. The posture maintenance through CCT was shown to be the economical way that contributed the low metabolic rate of echinoderms.

Report

(6 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (60 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (35 results) Book (7 results) Remarks (2 results)

  • [Journal Article] Softenin, a novel protein that softens the connective tissue of sea cucumbers through inhibiting interaction between collagen fibrils2014

    • Author(s)
      Takehana, Y., Yamada, A., Tamori, M. and Motokawa, T
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 1 Pages: e85644-e85644

    • DOI

      10.1371/journal.pone.0085644

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Softenin, a novel protein that softens the connective tissue of sea cucumbers through inhibiting interaction between collagen fibrils.2014

    • Author(s)
      Takehana, Y., Yamada, A., Tamori, M., and Motokawa, T.
    • Journal Title

      PLOS ONE

      Volume: 9

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Energy expenditure associated with softening and stiffening of echinoderm connective tissue2012

    • Author(s)
      Motokawa, T., Sato, E. and Umeyama, K
    • Journal Title

      Biol. Bull

      Volume: 222 Pages: 150-157

    • URL

      http://www.biolbull.org

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Energy expenditure associated with softening and stiffening of echinoderm connective tissue.2012

    • Author(s)
      Motokawa, T.
    • Journal Title

      Biol. Bull

      Volume: 222 Pages: 150-157

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Do regular sea urchins show preference in which part of the body they orient forward in their walk?2012

    • Author(s)
      Yoshimura, K.
    • Journal Title

      Marine Biol.

      Volume: 159 Pages: 959-965

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical mutability in connective tissue of starfish body wall2011

    • Author(s)
      Motokawa, T
    • Journal Title

      Biol. Bull

      Volume: 221 Pages: 280-289

    • URL

      http://www.biolbull.org

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical mutability in connective tissue of starfish body wall2011

    • Author(s)
      Motokawa, T.
    • Journal Title

      Biological Bulletin

      Volume: 221 Pages: 280-289

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue2010

    • Author(s)
      Yamada, A., Tamori, M., Iketani, T., Oiwa K. and Motokawa, T
    • Journal Title

      J. Exp. Biology

      Volume: 213 Issue: 20 Pages: 3416-3422

    • DOI

      10.1242/jeb.044149

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that water exudes when holothurian connective tissue stiffens2010

    • Author(s)
      Tamori, M., Takemae, C. and Motokawa, T
    • Journal Title

      J. Exp. Biol

      Volume: 213 Issue: 11 Pages: 1960-1966

    • DOI

      10.1242/jeb.038505

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue.2010

    • Author(s)
      Yamada, A., Tamori, M., Iketani, T., Oiwa K., Motokawa T.
    • Journal Title

      J.Exp.Biology

      Volume: 213 Pages: 3416-3422

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bilaterality in the regular sea urchin Anthocidaris crassispina is relatedto efficient defense not to efficient locomotion.2010

    • Author(s)
      Yoshimura, K., Motokawa, T.
    • Journal Title

      Marine Biology

      Volume: 157 Pages: 2475-2488

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that water exudes when holothurian connective tissue stiffens.2010

    • Author(s)
      Tamori, M., Takemae, C., Motokawa, T.
    • Journal Title

      J.Exp.Biology

      Volume: 213 Pages: 1960-1966

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that water exudes when holothurian connective tissue stiffens.2010

    • Author(s)
      Tamori, M.
    • Journal Title

      Journal of Experimental Biology (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reproductive biology and early life history of the hermaphroditic feather star Dorometra sesokonis (Echinodermata : Crinoidea)2010

    • Author(s)
      Obuchi, J.
    • Journal Title

      Marine Biology (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low oxygen consumption and high body content of catch connective tissue in body contribute to low metabolic rate of sea cucumbers2009

    • Author(s)
      Takemae, N., Nakaya, F., and Motokawa, T
    • Journal Title

      Biol. Bull

      Volume: 216 Pages: 45-54

    • URL

      http://www.biolbull.org

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low oxygen consumption and high body content of connective tissue contribute to low metabolic rate of sea cucmbers.2009

    • Author(s)
      Takemae, N
    • Journal Title

      Biological Bulletin 216

      Pages: 45-54

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Connective tissues in echinoderm animals that can reversibly change their stiffness2014

    • Author(s)
      Yamada, A, Takehana,Y, Tamori, M, and Motokawa, T
    • Organizer
      American Biophysical Society
    • Place of Presentation
      San Francisco, U.S.A
    • Year and Date
      2014-02-15
    • Related Report
      2013 Final Research Report
  • [Presentation] A-sea cucumber derived novel protein that softens the cell-disrupted catch connective tissue through inhibiting the interaction between collagen fibrils2014

    • Author(s)
      Takehana,Y, Yamada, A, Tamori, M, and Motokawa, T
    • Organizer
      American Biophysical Society
    • Place of Presentation
      San Francisco, U.S.A
    • Year and Date
      2014-02-15
    • Related Report
      2013 Final Research Report
  • [Presentation] A sea-cucumber derived novel protein that softens the cell-disrupted catch connective tissue through inhibiting the interaction between collagen fibrils.2014

    • Author(s)
      Takehana, Y., Yamada, A., Tamori, M., and Motokawa, T.
    • Organizer
      American Biophysical Society
    • Place of Presentation
      Moscone Center, San Francisco
    • Related Report
      2013 Annual Research Report
  • [Presentation] Connective tissues in echinoderm animals that can reversibly change their stiffness and their stiffening protein factors.2014

    • Author(s)
      Yamada, A.,Tahehana, Y., Tamori, M., and Motokawa, T.
    • Organizer
      American Biophysical Society
    • Place of Presentation
      Moscone Center, San Francisco
    • Related Report
      2013 Annual Research Report
  • [Presentation] The bounce in a seastar's step: classifying gaits in underwater legged locomotion.2014

    • Author(s)
      Ellers, O., Johnson,A., Guttenplan, K., and Motokawa, T.
    • Organizer
      Society for Integrative and Comparative Biology
    • Place of Presentation
      Hilton Hotel, Austin
    • Related Report
      2013 Annual Research Report
  • [Presentation] ガンガゼにおけるキャッチ結合組織と棘筋の協調的神経支配2013

    • Author(s)
      渕上芳朗、本川達雄
    • Organizer
      日本棘皮動物研究集会
    • Place of Presentation
      富山大学
    • Year and Date
      2013-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] シカクナマコの体壁真皮を軟化させるタンパク質の発見2013

    • Author(s)
      竹花康弘、山田章、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-09-28
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ウニにおける神経ペプチド候補物質NGFFFamideとNGIWYamideの効果と局在2013

    • Author(s)
      白木智之、田守正樹、本川達雄
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2013-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] キャッチ結合組織(ナマコ真皮)を軟化させる因子の探求2013

    • Author(s)
      竹花康弘、田守正樹、本川達雄、山田章
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2013-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular mechanisms of reversible changes in stiffness of echinoderm collagenous"catch"connective tissues2012

    • Author(s)
      Yamada, A, Takehana,Y, Tamori, M, and Motokawa, T
    • Organizer
      日本生物物理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ウFGFFFamide :ウニのキャッチ結合組織を支配する神経ペプチド2012

    • Author(s)
      白木智之、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] FGFFFamide:ウニのキャッチ結合組織を支配する神経ペプチド2012

    • Author(s)
      白木智之
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Locomotion in the sea urchin Diadema setosum2012

    • Author(s)
      Yoshimura, K.
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      総合研究大学院大学(葉山町)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Life history of a hermaphroditic feather star, Dorometra sesokonis (Crinoidea: Comatulida).2012

    • Author(s)
      Obuchi, M.
    • Organizer
      14th International Echinoderm Conference
    • Place of Presentation
      Brussels(Belgium)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ガンガゼDiadema setosumの歩行における棘と管足の役割について2011

    • Author(s)
      幾原綾美・吉村和也・本川達雄
    • Organizer
      日本棘皮動物研究集会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトデ体壁のキャッチ結合組織2011

    • Author(s)
      本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      大雪アリーナ
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞を破壊したナマコ真皮に対する真皮抽出物の効果2011

    • Author(s)
      竹花康弘、池谷知昭、山田章、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトデ体壁のキャッチ結合組織2011

    • Author(s)
      本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 楕円形の正形類ウニも壁に接触していた部分を先頭にして進む2011

    • Author(s)
      吉村和也・本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      大雪アリーナ
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞を破壊したナマコ真皮に対する真皮抽出物の効果2011

    • Author(s)
      竹花康弘・池谷知明・山田章・田守正樹・本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      大雪アリーナ
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] ラッパウニの巨大叉棘中にみられるダンベル状の骨片について2010

    • Author(s)
      本川達雄
    • Organizer
      日本棘皮動物研究集会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] シカクナマコ結合組織の硬化および軟化に伴うエネルギー消費2010

    • Author(s)
      佐藤絵里子、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-09-25
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] イオン液体を用いたラッパウニ叉棘のSEM観察2010

    • Author(s)
      山村吉信・幸喜順・本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] バフンウニの進行方向には"記憶様"の過程が関与する2010

    • Author(s)
      吉村和也・本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Why not walk faster, under water?2010

    • Author(s)
      Ellers, O
    • Organizer
      Society for Integrative and Comparative Biology
    • Place of Presentation
      Seattle, U.S.A.
    • Year and Date
      2010-01-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウミユリ巻枝の神経2009

    • Author(s)
      山本玄
    • Organizer
      棘皮動物研究集会
    • Place of Presentation
      東京
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] サンゴ礁「ガレ場」に生息するセソコヒメウミシダの繁殖生態2009

    • Author(s)
      小渕正美
    • Organizer
      日本サンゴ礁学会
    • Place of Presentation
      沖縄県本部町
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 時間って何だろう?2009

    • Author(s)
      本川達雄
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウニ(ガンガゼ)の歩行における棘と管足の働き2009

    • Author(s)
      吉村和也
    • Organizer
      日本動物学会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生物から環境2009

    • Author(s)
      本川達雄
    • Organizer
      ナチュラルサイエンス学会
    • Place of Presentation
      仙台
    • Year and Date
      2009-07-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] ガンガゼにおけるキャッチ結合組織と棘筋の協調的神経支配

    • Author(s)
      渕上芳朗,本川達雄
    • Organizer
      棘皮動物研究集会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ウニ(ガンガゼ)の歩行動態

    • Author(s)
      笹岡陽介、本川達雄
    • Organizer
      棘皮動物研究集会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] バフンウニにおける歩行と放射神経の関係

    • Author(s)
      釣巻創、本川達雄
    • Organizer
      棘皮動物研究集会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] キャッチ結合組織(ナマコ真皮)を軟化させる因子の探求

    • Author(s)
      竹花康弘
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Does the starfish Protoreaster nodosum use a pendular gait?

    • Author(s)
      Ellers, O.
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学(東京都)
    • Related Report
      2012 Annual Research Report
  • [Book] 生物学的文明論2011

    • Author(s)
      本川達雄
    • Total Pages
      250
    • Publisher
      新潮社
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Book] 環境保護は日本の歴史に秘策あり!2010

    • Author(s)
      松井孝典・養老孟司・安田喜憲・本川達雄・大井玄
    • Total Pages
      125
    • Publisher
      文藝春秋社
    • Related Report
      2010 Annual Research Report
  • [Book] 世界平和はナマコとともに2009

    • Author(s)
      本川達雄
    • Total Pages
      287
    • Publisher
      阪急コミュニケーションズ
    • Related Report
      2013 Final Research Report 2009 Annual Research Report
  • [Book] ウニ学2009

    • Author(s)
      本川達雄, 他
    • Total Pages
      462
    • Publisher
      東海大学出版会
    • Related Report
      2013 Final Research Report
  • [Book] ウニ学2009

    • Author(s)
      本川達雄
    • Total Pages
      462
    • Publisher
      東海大学出版会
    • Related Report
      2009 Annual Research Report
  • [Book] 自己組織化ハンドブック2009

    • Author(s)
      国武豊喜
    • Total Pages
      940
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2009 Annual Research Report
  • [Book] さまざまな神経系をもつ動物たち2009

    • Author(s)
      小泉修
    • Total Pages
      239
    • Publisher
      共立出版
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://home.t01.itscom.net/motokawa/

    • Related Report
      2013 Final Research Report
  • [Remarks] 本川達雄のホームページ

    • URL

      http://home.t01.itscom.net/motokawa/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

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