| Project/Area Number |
21570109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Chiba University |
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Principal Investigator |
YONEZAWA Naoto 千葉大学, 大学院・理学研究科, 准教授 (80212314)
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| Co-Investigator(Renkei-kenkyūsha) |
TANOKURA Masaru 東京大学, 農学生命科学研究科, 教授 (60136786)
YANAGIDA Mitsuaki 順天堂大学, 医学研究所, 准教授 (80365569)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 糖タンパク質 / 受精 / 細胞外マトリクス / 透明帯 / 細胞外マトリックス |
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| Research Abstract |
Protein compositions of mammalian egg coats are different among mammals, and therefore sperm recognition mechanism might be different among mammals. In pigs and cows, both egg coats are composed of ZP2, ZP3, and ZP4 glycoproteins and ZP3/ZP4 complex shows sperm-binding activity. Therefore, we speculated that sperm recognition mechanisms are similar between pigs and cows. In this project, we found that in cows N-terminal region of ZP3 and C-terminal region of ZP4 were involved in sperm-binding activity. On the other hand, in pigs C-terminal region of ZP3 and N-terminal region of ZP4 were involved in the binding activity. This difference in sperm-binding sites suggests difference in supramolecular structures between pig and bovine egg coats. This hypothesis is to be investigated.
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