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Clarification of the physiological role of diacylglycerol in E. coli

Research Project

Project/Area Number 21570110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionIwate University

Principal Investigator

NISHIYAMA Ken-ichi  岩手大学, 農学部, 教授 (80291334)

Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsMPIase / SecG / ジアシルグリセロール / タンパク質膜挿入 / 再構成 / 大腸菌 / 自発的膜挿入 / 低温感受性 / 機能的膜タンパク質合成
Research Abstract

To clarify the physiological role of diacylglycerol in E. coli, ~10 genes, which may be involved in diacylglycerol biosynthesis, were listed up, and then constructed multiply knock-out strains. Resultantly, an eightfold knock-out strain could be constructed, of which the growth was cold-sensitive and the diacylglycerol content was greatly reduced. However, the depletion was not complete. On the other hand, we determined the structure and analyzed the function of glycolipid MPIase involved in protein integration into membranes.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (27 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation2012

    • Author(s)
      Moser, M., Nagamori, S., Huber, M., Tokuda, H., Nishiyama, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: (in press) Issue: 24 Pages: 9734-9739

    • DOI

      10.1073/pnas.1303160110

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Nishiyama, K., Maeda,M., Yanagisawa,K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H., Shimamoto, K.
    • Journal Title

      Nat. Commun

      Volume: 3 Issue: 1 Pages: 1-10

    • DOI

      10.1038/ncomms2267

    • NAID

      120007179832

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion2012

    • Author(s)
      Morita, K., Tokuda, H. and Nishiyama, K.
    • Journal Title

      J. Biol. Chem

      Volume: 287巻 Issue: 1 Pages: 455-464

    • DOI

      10.1074/jbc.m111.301754

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion2012

    • Author(s)
      Nishiyama, K (Morita, K)
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 455-464

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of a highly translocation-competent proOmpA/SecB complex.2010

    • Author(s)
      Nishiyama, K., and Tokuda, H.
    • Journal Title

      Protein Sci.

      Volume: 19 Issue: 12 Pages: 2402-2408

    • DOI

      10.1002/pro.520

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel complete reconstitution system for membrane integration of the simplest membrane protein.2010

    • Author(s)
      Nishiyama, K., Maeda, M., Abe, M., Kanamori, T., Shimamoto, K., Kusumoto, S., Ueda, T., and Tokuda, H.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 394 Issue: 3 Pages: 733-736

    • DOI

      10.1016/j.bbrc.2010.03.061

    • NAID

      120002439245

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel complete reconstitution system for membrane integration of the simplest membrane protein2010

    • Author(s)
      西山賢一
    • Journal Title

      Biochem. Biophys. Res.Commun.

      Volume: 394 Pages: 733-736

    • NAID

      120002439245

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of a highly translocation-competent proOmpA/SecB complex2010

    • Author(s)
      西山賢一
    • Journal Title

      Protein Science

      Volume: 19 Pages: 2402-2408

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel complete reconstitution system for membrane integration of the simplest membrane protein2010

    • Author(s)
      西山賢一
    • Journal Title

      Biochem.Biophys.Res.Commun. 394(印刷中)

    • NAID

      120002439245

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a functional in vitro integration system for an integral membrane protein, SecG.2009

    • Author(s)
      Nishiyama, K., and Tokuda, H.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 390 Issue: 3 Pages: 920-924

    • DOI

      10.1016/j.bbrc.2009.10.078

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a functional in vitro integration system for an integral membrane protein, SecG2009

    • Author(s)
      西山賢一
    • Journal Title

      Biochem.Biophys.Res.Commun. 390

      Pages: 920-924

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌のタンパク質膜挿入に必須の糖脂質酵素(Glycolipozyme) MPIaseの構造と機能2013

    • Author(s)
      西山賢一
    • Organizer
      日本農芸化学会2013年度大会 シンポジウム「グラム陰性細菌細胞表層構造の形成機構とバイオテクノロジー」
    • Place of Presentation
      東北大学川内北キャンパス(宮城県)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌のタンパク質膜挿入に必須の糖脂質酵素(Glycolipozyme) MPIaseの構造と機能2013

    • Author(s)
      西山賢一
    • Organizer
      日本農芸化学会2013年度大会 シンポジウム「グラム陰性細菌細胞表層構造の形成機構とバイオテクノロジー」
    • Place of Presentation
      宮城県仙台市、東北大学川内北キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大腸菌のタンパク質膜挿入に必須の糖脂質酵素(Glycolipozyme) MPIaseの構造と機能2012

    • Author(s)
      西山賢一
    • Organizer
      第7回無細胞生命科学研究会
    • Place of Presentation
      愛媛大学南加記念ホール(愛媛県)
    • Year and Date
      2012-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質膜挿入に必須の糖脂質酵素MPIaseの構造と機能2012

    • Author(s)
      西山賢一
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      長浜ロイヤルホテル(滋賀県)
    • Year and Date
      2012-06-22
    • Related Report
      2012 Final Research Report
  • [Presentation] MPIase as a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Ken-ichi Nishiyama
    • Organizer
      Gordon Research Conference on Bacterial Cell Surface
    • Place of Presentation
      Mount Snow Resort, West Dover, VT, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質膜挿入に必須の糖脂質MPIaseとその標的タンパク質との相互作用解析2012

    • Author(s)
      Michael Moser(西山賢一)
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー~分子標的と活性制御~」第2回公開シンポジウム
    • Place of Presentation
      東京都、東京大学弥生講堂
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質膜挿入に必須の糖脂質酵素MPIaseの構造と機能2012

    • Author(s)
      西山賢一
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      滋賀県長浜市、長浜ロイヤルホテル
    • Related Report
      2012 Annual Research Report
  • [Presentation] MPIase as a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Ken-ichi Nishiyama
    • Organizer
      Gordon Research Conference on Bacterial Cell Surface
    • Place of Presentation
      Mount Snow Resort, Wset Dover, VT, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] MPIase, a novel glycolipozyme, is essential for topology inversion of SecG2012

    • Author(s)
      Michael Moser (Nishiyama, K)
    • Organizer
      The 1st International Symposium on Chemical Biology of Natural Products: Target ID and Regulation of Bioactivity
    • Place of Presentation
      京都市、京都センチュリーホテル
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structure and function of novel glycolipid characterized as membrane protein integrase2012

    • Author(s)
      Keiko Shimamoto (Nishiyama, K)
    • Organizer
      The 1st International Symposium on Chemical Biology of Natural Products: Target ID and Regulation of Bioactivity
    • Place of Presentation
      京都市、京都センチュリーホテル
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質膜挿入に必須の糖脂質酵素(Glycolipozyme)MPIase2012

    • Author(s)
      西山賢一
    • Organizer
      第7回 無細胞生命科学研究会プログラム
    • Place of Presentation
      愛媛県松山市、愛媛大学南加記念ホール
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質膜挿入・膜透過に関与する糖脂質MPIase の構造と機能2011

    • Author(s)
      西山賢一
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Year and Date
      2011-11-16
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質膜挿入に関与する新規複合糖脂質MPIaseの構造と機能2011

    • Author(s)
      西山賢一
    • Organizer
      第84回生化学会大会
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] MPIaseによるSecYEGの構造変化とSecGの膜内配向性反転2010

    • Author(s)
      西山賢一
    • Organizer
      第5回無細胞生命科学研究会
    • Place of Presentation
      岡山大学
    • Year and Date
      2010-09-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] MPIase, a novel glycolipid involved in membrane protein integration and preprotein translocation2010

    • Author(s)
      Ken-ichi Nishiyama
    • Organizer
      The 3rd International Symposium on Protein Community
    • Place of Presentation
      ホテル日航奈良(奈良)
    • Year and Date
      2010-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] MPIase, a novel glycolipid involved in membrane protein integration and preprotein translocation2010

    • Author(s)
      西山賢一
    • Organizer
      The 3rd International Symposiun on protein Community
    • Place of Presentation
      奈良、ホテル日航 招待講演
    • Year and Date
      2010-09-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] A novel complete reconstitution system for membrane integration of the simplest membrane protein2010

    • Author(s)
      Ken-ichi Nishiyama
    • Organizer
      Gordon Research Conference on Bacterial Cell Surfaces
    • Place of Presentation
      New London, NH, USA
    • Year and Date
      2010-06-30
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 機能的膜タンパク質のin vitro合成と膜挿入2009

    • Author(s)
      西山賢一
    • Organizer
      第4回無細胞生命科学研究会
    • Place of Presentation
      下呂市民会館(岐阜)
    • Year and Date
      2009-11-16
    • Related Report
      2012 Final Research Report 2009 Annual Research Report
  • [Presentation] 大腸菌におけるタンパク質膜挿入機構2009

    • Author(s)
      西山賢一
    • Organizer
      第6回21世紀大腸菌研究会
    • Place of Presentation
      KKRホテル熱海(静岡)
    • Year and Date
      2009-06-12
    • Related Report
      2012 Final Research Report 2009 Annual Research Report
  • [Presentation] The oligomeric structure of the E.coil SecYEG translocon is influenced by MPIase, a novel glycolipid

    • Author(s)
      西山賢一
    • Organizer
      第84回生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] タンパク質膜挿入に関与する新規複合糖脂質MPIaseの構造と機能

    • Author(s)
      西山賢一
    • Organizer
      第84回生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system

    • Author(s)
      西山賢一
    • Organizer
      第84回生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜タンパク質挿入に関わる新規糖脂質MPIaseの機能と構造

    • Author(s)
      西山賢一
    • Organizer
      第53回天然有機化合物討論会
    • Place of Presentation
      大阪国際センター(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell-Free Construction of Synthetic Biomembrane

    • Author(s)
      西山賢一
    • Organizer
      Annual Meeting of Cell Synthesis Research
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2011 Annual Research Report
  • [Presentation] タンパク質膜挿入・膜透過に関与する糖脂質MPIaseの構造と機能

    • Author(s)
      西山賢一
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 抗MPIase抗体を用いた糖脂質MPIaseの構造と機能解析

    • Author(s)
      西山賢一
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新しい因子:機能と構造

    • Author(s)
      西山賢一
    • Organizer
      第14回生命化学研究会
    • Place of Presentation
      ラフォーレ南紀白浜(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Book] 膜タンパク質の鍵は糖脂質にあり-すべての生体膜挿入に必須な因子を求めて-月刊化学2013

    • Author(s)
      西山賢一、島本啓子
    • Publisher
      化学同人
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://news7a1.atm.iwate-u.ac.jp/~sec/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://news7al.atm.iwate-u.ac.jp/~sec/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://news7a1.atm.iwate-u.ac.jp/~sec/

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 大腸菌膜タンパク質挿入因子2011

    • Inventor(s)
      楠本正一、島本啓子、前田将秀、西山賢一、徳田元、上田卓也、金森崇
    • Industrial Property Rights Holder
      サントリーホールディングス株式会社
    • Industrial Property Number
      2010-048520
    • Filing Date
      2011-03-05
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 大腸菌膜タンパク質挿入因子2010

    • Inventor(s)
      楠本正一, 島本啓子, 前田将秀, 西山賢一, 徳田元, 上田卓也, 金森崇
    • Industrial Property Rights Holder
      サントリーホールディングス株式会社
    • Industrial Property Number
      2010-048520
    • Filing Date
      2010-03-05
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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