Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Research Abstract |
We investigated the three-dimensional structure of the DNA polymerase B(PolB)-PCNA-DNA complex, which is the core components of the replisome, by single particle electron microscopy. Notably, besides the authentic interaction through a PCNA-interacting protein box(PIP-box), a novel contact was found between PolB and PCNA. The obtained molecular architecture of the complex, including the new contacts found in this work, provides clearer insights into the mechanism of switching between the two distinct modes of the complex.
|