Roles of AAA peroxins in peroxisome biogenesis

• Project/Area Number: 21570116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: TAMURA Shigehiko 九州大学, 大学院・理学研究院, 准教授 (90236753)
• Co-Investigator(Kenkyū-buntansha): FUJIKI Yukio 九州大学, 大学院・理学研究院, 教授 (70261237)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞小器官 / ペルオキシソーム / オルガネラ / AAAタンパク質 / ペルオキシン / ペルオキシソーム欠損症 / タンパク質相互作用 / 膜透過装置複合体

Research Abstract

The aim of this study is to investigate the molecular mechanism of peroxisome biogenesis. Pexl4p is a central component of the peroxisomal protein import machinery, in which the conserved Nterminal domain mediates dynamic interactions with Pex5p. We reported the crystal structure of the conserved Nterminal domain of Pexl4p with a three-helix bundle. Furthermore, AAA peroxins are most likely regulated in their peroxisomal localization onto Pex26p via conformational changes by the ATPase cycle, and modulatethe interaction between Pex26p and Pexl4p on peroxisome membrane.

Research Products

• [Journal Article] New insights into dynamic and functional assembly of the AAA peroxins, Pex1p and Pex6p, and their membrane receptor Pex26p in shuttling of PTS1-receptor Pex5p during peroxisome biogenesis (2012)
  • Author(s): Fujiki, Y., et al.
  • Journal Title: Biochimica et Biophysica Acta-Molecular Cell Research Volume: 1823 Issue: 1 Pages: 145-149
  • DOI: 10.1016/j.bbamcr.2011.10.012
  • Peer Reviewed
• [Journal Article] Recruiting mechanism of the AAA peroxins (2011)
  • Author(s): Nashiro, C., Kashiwag, A., Matsuzaki, T., Tamura, S. and Fujiki, Y.
  • Journal Title: Pexlp and Pex6p, to Pex26p on the peroxisomal membrane. Traffic Volume: 6 Pages: 774-788
• [Journal Article] Recruiting Mechanism of the AAA peroxins, Pex1p and Pex6p, to Pex26p on Peroxisome Membrane (2011)
  • Author(s): Nashiro, C., et al.
  • Journal Title: Traffic Volume: 12 Issue: 6 Pages: 774-788
  • DOI: 10.1111/j.1600-0854.2011.01182.x
  • Peer Reviewed
• [Journal Article] Recruiting Mechanism of the AAA peroxins, Pex1p and Pex6p, to Pex26p on Peroxisome Membrane. (2011)
  • Author(s): Nashiro, C., et.al.
  • Journal Title: Traffic Volume: (in press)
  • Peer Reviewed
• [Journal Article] Monomer-dimer transition of the conserved N-terminal domain of the mammalian peroxisomal matrix protein import receptor, Pex14p (2010)
  • Author(s): Su, J.R., Takeda, K., Tamura, S., Fujiki, Y. and Miki, K.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 394 Pages: 217-221
• [Journal Article] Monomer-dimer transition of the conserved N-terminal domain of the mammalian peroxisomal matorix protein import receptor, Pex14p (2010)
  • Author(s): Su, J.R, et.al.
  • Journal Title: Biochem.Biophys.Res.Commun. 394 Pages: 217-221
  • Peer Reviewed
• [Journal Article] Crystal structure of the conserved N-terminal domain of the peroxisomal matrix-protein-import receptor, Pexl4P (2009)
  • Author(s): Su JR., Takeda K., Tamura S., Fujiki Y. and Miki K.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 417-421
• [Journal Article] Crystal struture of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p (2009)
  • Author(s): Su, J.R, et.al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 417-421
  • Peer Reviewed
• [Presentation] ペルオキシソーム形成とその障害の分子メカニズム (2012)
  • Author(s): 田村茂彦
  • Organizer: 第6回先端医療薬学研究センター講演会(招待講演)
  • Place of Presentation: 岩手医科大学薬学部(岩手)
  • Year and Date: 2012-03-16
• [Presentation] ペルオキシソーム形成とその障害の分子メカニズム (2012)
  • Author(s): 田村茂彦
  • Organizer: 先端医療薬学研究センター講演会
  • Place of Presentation: 岩手医科大学薬学部(岩手)(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] AAA peroxins and their recruiter Pex26p modulate the peroxin interactions involved in peroxisomal protein import (2011)
  • Author(s): 田村茂彦
  • Organizer: 第9回国際AAAタンパク質会議
  • Place of Presentation: 熊本交通センターホテル(熊本)
  • Year and Date: 2011-11-08
• [Presentation] AAA peroxins and their recruiter Pex26p modulate the peroxin interactions involved in peroxisomal protein import (2011)
  • Author(s): 田村茂彦
  • Organizer: AAA国際シンポジウム
  • Place of Presentation: 熊本交通センターホテル(熊本)
  • Year and Date: 2011-11-08
• [Presentation] ナンセンス変異の抑制によるペルオキシソーム障害の回復メカニズム (2011)
  • Author(s): 田村茂彦
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-09-24
• [Presentation] ナンセンス変異の抑制によるペルオキシソーム障害の回復メカニズム (2011)
  • Author(s): 田村茂彦
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-09-24
• [Presentation] BN-PAGEによるべルオキシン複合体の解析 (2011)
  • Author(s): 田村茂彦
  • Organizer: 第11回蛋白質科学会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪)
  • Year and Date: 2011-06-09
• [Presentation] BN-PAGEによるペルオキシン複合体の解析 (2011)
  • Author(s): 田村茂彦
  • Organizer: 蛋白質科学会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪)
  • Year and Date: 2011-06-09
• [Presentation] AAAペルオキシンとPex26pによって制御されるペルオキシン相互作用 (2010)
  • Author(s): 田村茂彦
  • Organizer: 第83回日本生化学会・第33回分子生物学会合同大会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2010-12-08
• [Presentation] AAAペルオキシンとPex26pによって制御されるペルオキシン相互作用 (2010)
  • Author(s): 田村茂彦
  • Organizer: 日本生化学会・分子生物学会合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] Restoration of peroxisome biogenesis in peroxisomedeficient cells with a nonsense suppressor compound(G418) (2010)
  • Author(s): 田村茂彦
  • Organizer: 第3回タンパク質の社会に関する国際会議
  • Place of Presentation: ホテル日航奈良(奈良)
  • Year and Date: 2010-09-14
• [Presentation] Restoration of peroxisome biogenesis in peroxisome-deficient cells with a nonsense suppressor compound (G418). (2010)
  • Author(s): 田村茂彦
  • Organizer: 特定領域「タンパク質の社会」国際シンポジウム
  • Place of Presentation: ホテル日航奈良
  • Year and Date: 2010-09-14
• [Presentation] ペルオキシソーム形成におけるPex5pのダイナミズムとその制御システム (2009)
  • Author(s): 田村茂彦
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2009-10-21
• [Presentation] ペルオキシソーム形成におけるPex5pのダイナミズムとその制御システム (2009)
  • Author(s): 田村茂彦
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-21
• [Remarks]
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~taisha/