Elucidation of structure and function relationship in multiple-domain chitinases probed by a combination of X-ray crystallography, solution scattering, and molecular dynamics simulation
| Project/Area Number |
21570119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Masaki 東京薬科大学, 生命科学部, 教授 (90277252)
KEZUKA Yuichiro 岩手医科大学, 薬学部, 助教 (50397163)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | タンパク質 / X線構造解析 / 蛋白質 / X線結晶構造解析 / X線溶液散乱 / 分子動力学計算 / キチナーゼ / 酵素反応 / 糖鎖 / 分子認識 / 生物物理 |
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| Research Abstract |
We suggest a hypothetical binding model of chitin-binding domains of Cht2 and ChiC, members of glycoside hydrolase family 19, against crystallineα-chitin. This model indicates the difference in the binding specificity of two chitinases. In both chitinases, the conformational flexibility of the interdomain linker can be considered to cause the conformational extension and the variability of the domain arrangement. These results tempt us to speculate the following mechanism of chitin degradation. While ChBD binds to chitin chain and acts as an anchor, CatD degrades chitin chains within a defined region of the radius depending on linker length.
We determined and refined the crystal structures of the catalytic domain of chitinase D and its inhibitor complex. It is shown that conformational change of a substrate-binding loop is induced by the inhibitor binding.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Structure of full-length class I chitinase from rice revealed by X-ray crystallography and small-angle2010
Author(s)
Kezuka, Y., Kojima, M., Mizuno, R., Suzuki, K., Watanabe, T. & Nonaka, T
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Journal Title
X-ray scattering Proteins
Volume: 78
Pages: 2295-2305
Related Report
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