Structural and functional basis of the complex of mono-ADP-ribosylating toxin and substrate protein
Project/Area Number |
21570121
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
|
Research Institution | Kyoto Sangyo University (2010-2011) Tokushima Bunri University (2009) |
Principal Investigator |
TSUGE Hideaki 京都産業大学, 総合生命科学部, 教授 (40299342)
|
Co-Investigator(Renkei-kenkyūsha) |
SAKURAI Jun 徳島文理大学, 薬学部, 教授 (80029800)
NAGAHAMA Masahiro 徳島文理大学, 薬学部, 教授 (40164462)
ODA Masataka 徳島文理大学, 薬学部, 講師 (00412403)
|
Research Collaborator |
TSURUMURA Toshiharu 京都産業大学, 総合生命科学部, プロジェクト助教 (50450250)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | C3 / RhoA / Ia / アクチン / モノADPリボシル化 / 結晶構造解析 / 特異性 / 複合体結晶構造解析 / ADPリボシルトランスフェラーゼ / 細菌毒素 / X線結晶構造解析 / 複合体解析 / C3毒素 |
Research Abstract |
To reveal the structural and functional mechanism of C. perfringens iota toxin(Ia) and actin, we successfully crystallized and solved the structure of the apo-Ia-actin and apo-Ia. We revealed the structural change upon the ADP-ribosylation by comparing the structures together with NADH-Ia and βTAD-Ia-actin complex. C. Botulinum C3 toxin ADP-ribosylates RhoA Asn41. For the structure analysis of C3-RhoA complex, RhoA was expressed in E. coli and the stable mutant of RhoAF25N was obtained using His-tag and GST-tag, independently. We are currently searching the crystallization condition for the complex.
|
Report
(4 results)
Research Products
(45 results)