Development of automated visualization system for very fine protein crystals

• Project/Area Number: 21570124
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: IGARASHI Noriyuki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
• Co-Investigator(Renkei-kenkyūsha): WAKATSUKI Soichi 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (00332114) ; HIRAKI Masahiko 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 研究機関講師 (20282676) ; MATSUGAKI Naohiro 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (50342598) ; YAMADA Yusuke 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (20391708)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: X線結晶構造解析 / 可視化 / 測定の自動化技術

Research Abstract

We pursued development of automated visualization system for very fine protein crystals using edge enhancement of very fine crystals by controlling light direction. We completed development of elemental technology of micron-size visualization system and will do futher development in order to implement this system into fully-automated data collection system.

Research Products

• [Presentation] PF構造生物ビームラインにおける全自動回折実験システムの高度化と汎用化 (2012)
  • Author(s): 山田悠介、松垣直宏、平木雅彦、Leonard M. G. Chavas、五十嵐教之、若槻壮市
  • Organizer: 第25回日本放射光学会年会放射光科学合同シンポジウム
  • Place of Presentation: 鳥栖市
  • Year and Date: 2012-01-08
• [Presentation] Automation of data collection and processing at the Photon Factory macromolecular crystallography beamlines (2011)
  • Author(s): Yusuke Yamada, Masahiko Hiraki, Naohiro Matsugaki, Leonard M. G. Chavas, Noriyuki Igarashi and Soichi Wakatsuki
  • Organizer: A Special Symposium Celebrating the 40th Anniversary of the Protein Data Bank(PDB40)、Cold Spring Harbor
  • Place of Presentation: NY(US)
  • Year and Date: 2011-10-29